Abstract
Interleukin-3 (IL-3) is a cytokine that is expressed primarily in activated T cells. Here we identified an inducible T cell-specific enhancer 14 kb upstream of the IL-3 gene that responded to activation of T cell receptor signaling pathways. The IL-3 enhancer spanned an inducible cyclosporin A-sensitive DNase I-hypersensitive site found only in T cells. Four NFAT-like elements exist within the enhancer. The two most active NFAT-like elements were located at the center of the DNase I-hypersensitive site. One of these NFAT-like elements encompassed overlapping Oct- and NFATp/c-binding sites, which functioned in a highly synergistic manner. We suggest that the T cell-specific expression of the IL-3 gene is partly controlled through the enhancer by cooperation between Oct and NFAT family proteins.
Original language | English |
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Pages (from-to) | 175-85 |
Number of pages | 11 |
Journal | Immunity |
Volume | 6 |
Issue number | 2 |
Publication status | Published - Feb 1997 |
Keywords
- Base Sequence
- Cyclosporine
- DNA-Binding Proteins
- Deoxyribonuclease I
- Drug Synergism
- Enhancer Elements, Genetic
- Gene Expression Regulation
- HeLa Cells
- Homeodomain Proteins
- Host Cell Factor C1
- Humans
- Interleukin-3
- Jurkat Cells
- Molecular Sequence Data
- NFATC Transcription Factors
- Nuclear Proteins
- Octamer Transcription Factor-1
- Octamer Transcription Factor-2
- T-Lymphocytes
- Transcription Factors