A panel of monoclonal antibodies recognising GPI-anchored ADP-ribosyltransferase ART4, the carrier of the Dombrock Blood group antigens

I Parusel, S Kahl, G Glowacki, GR Halverson, ME Reid, A Schawalder, E Ortolan, A Funaro, F Malavasi, Deborah Hardie, S Halder, Christopher Buckley, F Haag, F Koch-Nolte

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21 Citations (Scopus)


ART4 (CD297) is a member of the family of toxin-related ADP-ribosyltransferases (ARTs) and is the carrier of the Dombrock blood group alloantigens (Do). Two mouse monoclonal antibodies (MIMA-52 and MIMA-53), and two rat monoclonal antibodies (N0NI-B4 and NONI-1363) were obtained following immunization of mice with human Do/ART4-transfected cells and of rats with human Do/ART4 cDNA, respectively. All four mAbs recognize Do/ART4-transfected Jurkat cells but not untransfected cells by FACS analysis. Staining of Do/ART4-transfected cells by these mAbs was reduced following treatment of cells with PI-PLC, confirming that Do/ART4 is anchored in the cell membrane by linkage to glycosylphosphatidylinositol as predicted from its amino acid sequence. The four mAbs did not react with Gy(a-) (Dombrock null) erythrocytes but agglutinated other red blood cells. By flow cytometric analysis, all mAbs reacted prominently with erythrocytes, and weakly with peripheral blood monocytes and splenic macrophages. but not with B-lymphocytes or T-lymphocytes. The mAbs reacted weakly also with human umbilical vein endothelial cells and the basophilic leukemia KU-812. Immunohistology revealed staining of epithelia and endothelia, on sections of tonsils. In FACS analyses NONI-1314 competed with MIMA-52 for binding to Do/ART4-transfected cells and erythrocytes, whereas NONI-1363 competed with MIMA-53. Neither of the mAbs reacted with mouse ART4-transfected cells, but NONI-B63 and MIMA-53 did react with a mouse/human ART4 chimera, indicating that the epitope recognized by these mAbs lies in the C-terminal half of the protein. (c) 2005 Elsevier Inc. All rights reserved.
Original languageEnglish
Pages (from-to)59-65
Number of pages7
JournalCellular Immunology
Issue number1-2
Publication statusPublished - 1 Jul 2005
Event8th International Workshop on Human Leucocyte Differentiation Antigens, 2004. Adelaide, Australia -
Duration: 1 Oct 2005 → …


  • genetic immunization
  • ART4
  • Dombrock
  • blood group antigen
  • monoclonal antibodies
  • GPI-anchored molecule


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