A multicassette Gateway vector set for high throughput and comparative analyses in ciona and vertebrate embryos

Agnès Roure, Ute Rothbächer, François Robin, Eva Kalmar, Giustina Ferone, Clément Lamy, Caterina Missero, Ferenc Mueller, Patrick Lemaire

Research output: Contribution to journalArticlepeer-review

90 Citations (Scopus)

Abstract

BACKGROUND: The past few years have seen a vast increase in the amount of genomic data available for a growing number of taxa, including sets of full length cDNA clones and cis-regulatory sequences. Large scale cross-species comparisons of protein function and cis-regulatory sequences may help to understand the emergence of specific traits during evolution.

PRINCIPAL FINDINGS: To facilitate such comparisons, we developed a Gateway compatible vector set, which can be used to systematically dissect cis-regulatory sequences, and overexpress wild type or tagged proteins in a variety of chordate systems. It was developed and first characterised in the embryos of the ascidian Ciona intestinalis, in which large scale analyses are easier to perform than in vertebrates, owing to the very efficient embryo electroporation protocol available in this organism. Its use was then extended to fish embryos and cultured mammalian cells.

CONCLUSION: This versatile vector set opens the way to the mid- to large-scale comparative analyses of protein function and cis-regulatory sequences across chordate evolution. A complete user manual is provided as supplemental material.

Original languageEnglish
Pages (from-to)e916
JournalPLoS ONE
Volume2
Issue number9
DOIs
Publication statusPublished - 19 Sept 2007

Keywords

  • Animals
  • Base Sequence
  • Cell Line
  • Ciona intestinalis/embryology
  • DNA Primers
  • DNA, Complementary
  • Open Reading Frames
  • Regulatory Sequences, Nucleic Acid
  • Vertebrates/embryology

Fingerprint

Dive into the research topics of 'A multicassette Gateway vector set for high throughput and comparative analyses in ciona and vertebrate embryos'. Together they form a unique fingerprint.

Cite this