13C-detection in RNA bases: revealing structure-chemical shift relationships

Christophe Farès; Irene Amata; Teresa Carlomagno

doi:10.1021/ja0727417

13C-detection in RNA bases: revealing structure-chemical shift relationships

Christophe Farès, Irene Amata, Teresa Carlomagno

Biosciences

Research output: Contribution to journal › Article › peer-review

Abstract

The chemical shifts of the unprotonated carbons in the proton-deficient nucleobases of RNA are rarely reported, despite the valuable information that they contain about base-pairing and base-stacking. We have developed 13C-detected 2D-experiments to identify the unprotonated 13C in the RNA bases and have assigned all the base nuclei of uniformly 13C,15N-labeled HIV-2 TAR-RNA. The 13C chemical shift distributions revealed perturbations correlated with the base-pairing and base-stacking properties of all four base-types. From this work, we conclude that the information contained in the chemical shift perturbations within the base rings can provide valuable restraint information for solving RNA structures, especially in conformational averaged regions, where NOE-based information is not available.

Original language	English
Pages (from-to)	15814-23
Number of pages	10
Journal	Journal of the American Chemical Society
Volume	129
Issue number	51
DOIs	https://doi.org/10.1021/ja0727417
Publication status	Published - 26 Dec 2007

Keywords

Base Sequence
Carbon Isotopes/analysis
Nuclear Magnetic Resonance, Biomolecular
Nucleic Acid Conformation
RNA/chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/ja0727417

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title = "13C-detection in RNA bases: revealing structure-chemical shift relationships",

abstract = "The chemical shifts of the unprotonated carbons in the proton-deficient nucleobases of RNA are rarely reported, despite the valuable information that they contain about base-pairing and base-stacking. We have developed 13C-detected 2D-experiments to identify the unprotonated 13C in the RNA bases and have assigned all the base nuclei of uniformly 13C,15N-labeled HIV-2 TAR-RNA. The 13C chemical shift distributions revealed perturbations correlated with the base-pairing and base-stacking properties of all four base-types. From this work, we conclude that the information contained in the chemical shift perturbations within the base rings can provide valuable restraint information for solving RNA structures, especially in conformational averaged regions, where NOE-based information is not available.",

keywords = "Base Sequence, Carbon Isotopes/analysis, Nuclear Magnetic Resonance, Biomolecular, Nucleic Acid Conformation, RNA/chemistry",

author = "Christophe Far{\`e}s and Irene Amata and Teresa Carlomagno",

year = "2007",

month = dec,

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doi = "10.1021/ja0727417",

language = "English",

volume = "129",

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T1 - 13C-detection in RNA bases

T2 - revealing structure-chemical shift relationships

AU - Farès, Christophe

AU - Amata, Irene

AU - Carlomagno, Teresa

PY - 2007/12/26

Y1 - 2007/12/26

N2 - The chemical shifts of the unprotonated carbons in the proton-deficient nucleobases of RNA are rarely reported, despite the valuable information that they contain about base-pairing and base-stacking. We have developed 13C-detected 2D-experiments to identify the unprotonated 13C in the RNA bases and have assigned all the base nuclei of uniformly 13C,15N-labeled HIV-2 TAR-RNA. The 13C chemical shift distributions revealed perturbations correlated with the base-pairing and base-stacking properties of all four base-types. From this work, we conclude that the information contained in the chemical shift perturbations within the base rings can provide valuable restraint information for solving RNA structures, especially in conformational averaged regions, where NOE-based information is not available.

AB - The chemical shifts of the unprotonated carbons in the proton-deficient nucleobases of RNA are rarely reported, despite the valuable information that they contain about base-pairing and base-stacking. We have developed 13C-detected 2D-experiments to identify the unprotonated 13C in the RNA bases and have assigned all the base nuclei of uniformly 13C,15N-labeled HIV-2 TAR-RNA. The 13C chemical shift distributions revealed perturbations correlated with the base-pairing and base-stacking properties of all four base-types. From this work, we conclude that the information contained in the chemical shift perturbations within the base rings can provide valuable restraint information for solving RNA structures, especially in conformational averaged regions, where NOE-based information is not available.

KW - Base Sequence

KW - Carbon Isotopes/analysis

KW - Nuclear Magnetic Resonance, Biomolecular

KW - Nucleic Acid Conformation

KW - RNA/chemistry

U2 - 10.1021/ja0727417

DO - 10.1021/ja0727417

M3 - Article

C2 - 18052161

SN - 0002-7863

VL - 129

SP - 15814

EP - 15823

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 51

ER -

13C-detection in RNA bases: revealing structure-chemical shift relationships

Abstract

Keywords

UN SDGs

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