Zinc dependence of zinT (yodA) mutants and binding of zinc, cadmium and mercury by ZinT.

Research output: Contribution to journalArticle

Authors

Colleges, School and Institutes

Abstract

ZinT (B1973), previously known as YodA, was originally characterised as a cadmium-induced periplasmic protein under the regulation of Fur and SoxS. Here we describe a decrease in zinT transcript in response to elevated copper concentrations and the zinc and copper dependent phenotype of a DeltazinT strain. Cadmium sensitivity of the DeltazinT strain was not observed. We demonstrate the binding of nickel, zinc, cadmium, and mercury, but not cobalt, copper, iron, and manganese, to purified ZinT using mass spectrometry. This and previous studies support the hypothesis that ZinT plays a role in zinc homeostasis and is required for growth under zinc limited conditions, suggesting that ZinT is either a periplasmic zinc chaperone or is involved in zinc import. Limited metal ion discrimination results in regulation of PzinT in a non-specific manner, which is mirrored in the binding of several different heavy metals by ZinT.

Details

Original languageEnglish
Pages (from-to)66-71
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume364
Issue number1
Publication statusPublished - 7 Dec 2007

Keywords

  • ZinT, zinc import, cadmium, YodA, mercury