Yeast transformation to generate high-yielding clones.

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There are several ways to introduce non-native DNA into yeast cells, including chemical transformation and electroporation. Methods for both of these procedures are outlined in this chapter. Both methods permit the uptake of DNA from the environment through yeast cell membranes and this DNA can be episomally maintained or integrated into the host genome. However, yeast cells must first be made competent to permit passive entry of the DNA and various methods are outlined in this chapter to facilitate this. All of the described methods can be applied in combination with antibiotic or auxotrophic selection pressure.


Original languageEnglish
Pages (from-to)57-63
Number of pages7
JournalMethods in molecular biology
Publication statusPublished - 1 Jan 2012