Wolbachia modulates lipid metabolism in Aedes albopictus mosquito cells

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Wolbachia modulates lipid metabolism in Aedes albopictus mosquito cells. / Molloy, Jennifer C.; Sommer, Ulf; Viant, Mark R.; Sinkins, Steven P.

In: Applied and Environmental Microbiology, Vol. 82, No. 10, 05.2016, p. 3109-3120.

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@article{bab8f287ffa64888b08d4f784f89e8be,
title = "Wolbachia modulates lipid metabolism in Aedes albopictus mosquito cells",
abstract = "Certain strains of the intracellular endosymbiont Wolbachia can strongly inhibit or block the transmission of viruses such as dengue virus (DENV) by Aedes mosquitoes, and the mechanisms responsible are still not well understood. Direct infusion and liquid chromatography-Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry-based lipidomics analyses were conducted using Aedes albopictus Aa23 cells that were infected with the wMel and wMelPop strains of Wolbachia in comparison to uninfected Aa23-T cells. Substantial shifts in the cellular lipid profile were apparent in the presence of Wolbachia. Most significantly, almost all sphingolipid classes were depleted, and some reductions in diacylglycerols and phosphatidylcholines were also observed. These lipid classes have previously been shown to be selectively enriched in DENV-infected mosquito cells, suggesting that Wolbachia may produce a cellular lipid environment that is antagonistic to viral replication. The data improve our understanding of the intracellular interactions between Wolbachia and mosquitoes.",
author = "Molloy, {Jennifer C.} and Ulf Sommer and Viant, {Mark R.} and Sinkins, {Steven P.}",
year = "2016",
month = may,
doi = "10.1128/AEM.00275-16",
language = "English",
volume = "82",
pages = "3109--3120",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "10",

}

RIS

TY - JOUR

T1 - Wolbachia modulates lipid metabolism in Aedes albopictus mosquito cells

AU - Molloy, Jennifer C.

AU - Sommer, Ulf

AU - Viant, Mark R.

AU - Sinkins, Steven P.

PY - 2016/5

Y1 - 2016/5

N2 - Certain strains of the intracellular endosymbiont Wolbachia can strongly inhibit or block the transmission of viruses such as dengue virus (DENV) by Aedes mosquitoes, and the mechanisms responsible are still not well understood. Direct infusion and liquid chromatography-Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry-based lipidomics analyses were conducted using Aedes albopictus Aa23 cells that were infected with the wMel and wMelPop strains of Wolbachia in comparison to uninfected Aa23-T cells. Substantial shifts in the cellular lipid profile were apparent in the presence of Wolbachia. Most significantly, almost all sphingolipid classes were depleted, and some reductions in diacylglycerols and phosphatidylcholines were also observed. These lipid classes have previously been shown to be selectively enriched in DENV-infected mosquito cells, suggesting that Wolbachia may produce a cellular lipid environment that is antagonistic to viral replication. The data improve our understanding of the intracellular interactions between Wolbachia and mosquitoes.

AB - Certain strains of the intracellular endosymbiont Wolbachia can strongly inhibit or block the transmission of viruses such as dengue virus (DENV) by Aedes mosquitoes, and the mechanisms responsible are still not well understood. Direct infusion and liquid chromatography-Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry-based lipidomics analyses were conducted using Aedes albopictus Aa23 cells that were infected with the wMel and wMelPop strains of Wolbachia in comparison to uninfected Aa23-T cells. Substantial shifts in the cellular lipid profile were apparent in the presence of Wolbachia. Most significantly, almost all sphingolipid classes were depleted, and some reductions in diacylglycerols and phosphatidylcholines were also observed. These lipid classes have previously been shown to be selectively enriched in DENV-infected mosquito cells, suggesting that Wolbachia may produce a cellular lipid environment that is antagonistic to viral replication. The data improve our understanding of the intracellular interactions between Wolbachia and mosquitoes.

UR - http://www.scopus.com/inward/record.url?scp=84966671717&partnerID=8YFLogxK

U2 - 10.1128/AEM.00275-16

DO - 10.1128/AEM.00275-16

M3 - Article

AN - SCOPUS:84966671717

VL - 82

SP - 3109

EP - 3120

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 10

ER -