Widespread suppression of intragenic transcription initiation by H-NS

Research output: Contribution to journalArticlepeer-review

Authors

  • Shivani S. Singh
  • Navjot Singh
  • Richard P. Bonocora
  • Devon M. Fitzgerald
  • Joseph T. Wade

Colleges, School and Institutes

External organisations

  • Wadsworth Center for Laboratories and Research
  • University at Albany State University of New York

Abstract

Widespread intragenic transcription initiation has been observed in many species. Here we show that the Escherichia coli ehxCABD operon contains numerous intragenic promoters in both sense and antisense orientations. Transcription from these promoters is silenced by the histone-like nucleoid structuring (H-NS) protein. On a genome-wide scale, we show that 46% of H-NS-suppressed transcripts in E. coli are intragenic in origin. Furthermore, many intergenic promoters repressed by H-NS are for noncoding RNAs (ncRNAs). Thus, a major overlooked function of H-NS is to prevent transcription of spurious RNA. Our data provide a molecular description for the toxicity of horizontally acquired DNA and explain how this is counteracted by H-NS.

Details

Original languageEnglish
Pages (from-to)214-219
Number of pages6
JournalGenes & Development
Volume28
Issue number3
Early online date21 Jan 2014
Publication statusPublished - 1 Feb 2014

Keywords

  • H-NS, Nucleoid, Pervasive transcription

ASJC Scopus subject areas