Wastewater used for urban agriculture in West Africa as a reservoir for antibacterial resistance dissemination

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Wastewater used for urban agriculture in West Africa as a reservoir for antibacterial resistance dissemination. / Bougnom, Blaise P.; Zongo, Cheikna; McNally, Alan; Ricci, Vito; Etoa, François X.; Thiele-Bruhn, Sören; Piddock, Laura J V.

In: Environmental Research, Vol. 168, 01.01.2019, p. 14-24.

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@article{d54e376d2a2f4c09b97bf68be96677b8,
title = "Wastewater used for urban agriculture in West Africa as a reservoir for antibacterial resistance dissemination",
abstract = "State of art metagenomics were used to investigate the microbial population, antibiotic resistance genes and plasmids of medical interest in wastewater used for urban agriculture in Ouagadougou (Burkina Faso). Wastewater samples were collected from three canals near agricultural fields in three neighbourhoods. Assessment of microbial population diversity revealed different microbial patterns among the different samples. Sequencing reads from the wastewaters revealed different functional specializations of microbial communities, with the predominance of carbohydrates and proteins metabolism functions. Eleven pathogen-specific and 56 orthologous virulence factor genes were detected in the wastewater samples. These virulence factors are usually found in human pathogens that cause gastroenteritis and/or diarrhoea. A wide range of antibiotic resistance genes was identified; 81 are transmissible by mobile genetic elements. These included seven different extended spectrum β-lactamase genes encoding synthesis of four enzyme families, including two metallo-β-lactamases (blaAIM-1 and blaGES-21). Ten different incompatibility groups of Enterobacteriaceae plasmid replicons (ColE, FIB, FIC, FII, P, Q, R, U, Y, and A/C), and 30 plasmid replicon types from Gram-positive bacteria. All are implicated in the wide distribution of antibiotic resistance genes. We conclude that wastewater used for urban agriculture in the city represents a high risk for spreading bacteria and antimicrobial resistance among humans and animals.",
keywords = "Antimicrobial resistance, Low and middle-income countries, Metagenomics, Urban agriculture",
author = "Bougnom, {Blaise P.} and Cheikna Zongo and Alan McNally and Vito Ricci and Etoa, {Fran{\c c}ois X.} and S{\"o}ren Thiele-Bruhn and Piddock, {Laura J V}",
note = "Copyright {\textcopyright} 2018 Elsevier Inc. All rights reserved.",
year = "2019",
month = jan,
day = "1",
doi = "10.1016/j.envres.2018.09.022",
language = "English",
volume = "168",
pages = "14--24",
journal = "Environmental Research",
issn = "0013-9351",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Wastewater used for urban agriculture in West Africa as a reservoir for antibacterial resistance dissemination

AU - Bougnom, Blaise P.

AU - Zongo, Cheikna

AU - McNally, Alan

AU - Ricci, Vito

AU - Etoa, François X.

AU - Thiele-Bruhn, Sören

AU - Piddock, Laura J V

N1 - Copyright © 2018 Elsevier Inc. All rights reserved.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - State of art metagenomics were used to investigate the microbial population, antibiotic resistance genes and plasmids of medical interest in wastewater used for urban agriculture in Ouagadougou (Burkina Faso). Wastewater samples were collected from three canals near agricultural fields in three neighbourhoods. Assessment of microbial population diversity revealed different microbial patterns among the different samples. Sequencing reads from the wastewaters revealed different functional specializations of microbial communities, with the predominance of carbohydrates and proteins metabolism functions. Eleven pathogen-specific and 56 orthologous virulence factor genes were detected in the wastewater samples. These virulence factors are usually found in human pathogens that cause gastroenteritis and/or diarrhoea. A wide range of antibiotic resistance genes was identified; 81 are transmissible by mobile genetic elements. These included seven different extended spectrum β-lactamase genes encoding synthesis of four enzyme families, including two metallo-β-lactamases (blaAIM-1 and blaGES-21). Ten different incompatibility groups of Enterobacteriaceae plasmid replicons (ColE, FIB, FIC, FII, P, Q, R, U, Y, and A/C), and 30 plasmid replicon types from Gram-positive bacteria. All are implicated in the wide distribution of antibiotic resistance genes. We conclude that wastewater used for urban agriculture in the city represents a high risk for spreading bacteria and antimicrobial resistance among humans and animals.

AB - State of art metagenomics were used to investigate the microbial population, antibiotic resistance genes and plasmids of medical interest in wastewater used for urban agriculture in Ouagadougou (Burkina Faso). Wastewater samples were collected from three canals near agricultural fields in three neighbourhoods. Assessment of microbial population diversity revealed different microbial patterns among the different samples. Sequencing reads from the wastewaters revealed different functional specializations of microbial communities, with the predominance of carbohydrates and proteins metabolism functions. Eleven pathogen-specific and 56 orthologous virulence factor genes were detected in the wastewater samples. These virulence factors are usually found in human pathogens that cause gastroenteritis and/or diarrhoea. A wide range of antibiotic resistance genes was identified; 81 are transmissible by mobile genetic elements. These included seven different extended spectrum β-lactamase genes encoding synthesis of four enzyme families, including two metallo-β-lactamases (blaAIM-1 and blaGES-21). Ten different incompatibility groups of Enterobacteriaceae plasmid replicons (ColE, FIB, FIC, FII, P, Q, R, U, Y, and A/C), and 30 plasmid replicon types from Gram-positive bacteria. All are implicated in the wide distribution of antibiotic resistance genes. We conclude that wastewater used for urban agriculture in the city represents a high risk for spreading bacteria and antimicrobial resistance among humans and animals.

KW - Antimicrobial resistance

KW - Low and middle-income countries

KW - Metagenomics

KW - Urban agriculture

UR - http://www.scopus.com/inward/record.url?scp=85053760577&partnerID=8YFLogxK

U2 - 10.1016/j.envres.2018.09.022

DO - 10.1016/j.envres.2018.09.022

M3 - Article

C2 - 30253312

VL - 168

SP - 14

EP - 24

JO - Environmental Research

JF - Environmental Research

SN - 0013-9351

ER -