Wastewater used for urban agriculture in West Africa as a reservoir for antibacterial resistance dissemination

Research output: Contribution to journalArticle

Authors

  • Cheikna Zongo
  • François X. Etoa
  • Sören Thiele-Bruhn

Colleges, School and Institutes

External organisations

  • Department of Microbiology, Faculty of Science, University of Yaounde
  • Department of Biochemistry and Microbiology, Ouaga University
  • Department of Soil Science, University of Trier

Abstract

State of art metagenomics were used to investigate the microbial population, antibiotic resistance genes and plasmids of medical interest in wastewater used for urban agriculture in Ouagadougou (Burkina Faso). Wastewater samples were collected from three canals near agricultural fields in three neighbourhoods. Assessment of microbial population diversity revealed different microbial patterns among the different samples. Sequencing reads from the wastewaters revealed different functional specializations of microbial communities, with the predominance of carbohydrates and proteins metabolism functions. Eleven pathogen-specific and 56 orthologous virulence factor genes were detected in the wastewater samples. These virulence factors are usually found in human pathogens that cause gastroenteritis and/or diarrhoea. A wide range of antibiotic resistance genes was identified; 81 are transmissible by mobile genetic elements. These included seven different extended spectrum β-lactamase genes encoding synthesis of four enzyme families, including two metallo-β-lactamases (blaAIM-1 and blaGES-21). Ten different incompatibility groups of Enterobacteriaceae plasmid replicons (ColE, FIB, FIC, FII, P, Q, R, U, Y, and A/C), and 30 plasmid replicon types from Gram-positive bacteria. All are implicated in the wide distribution of antibiotic resistance genes. We conclude that wastewater used for urban agriculture in the city represents a high risk for spreading bacteria and antimicrobial resistance among humans and animals.

Details

Original languageEnglish
Pages (from-to)14-24
Number of pages11
JournalEnvironmental Research
Volume168
Early online date18 Sep 2018
StatePublished - Jan 2019

Keywords

  • antimicrobial resistance, metagenomics, urban agriculture, low and middle-income countries