Wall material properties of yeast cells: Part 1. Cell measurements and compression experiments

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Wall material properties of yeast cells : Part 1. Cell measurements and compression experiments. / Smith, A. E.; Zhang, Z.; Thomas, C. R.

In: Chemical Engineering Science, Vol. 55, No. 11, 06.2000, p. 2031-2041.

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@article{bbfb539aafb4419d94b02be2b74ef1b4,
title = "Wall material properties of yeast cells: Part 1. Cell measurements and compression experiments",
abstract = "To interpret data from the compression experiment (micromanipulation), the initial radial stretch ratio and the wall thickness to cell radius ratio are required. Methods have been developed to measure these parameters for baker's yeast (Saccharomyces cerevisiae) prior to compression experiments. The initial radial stretch ratio was found using osmotic theory to interpret measurements of cell-volume change as a function of external osmotic pressure. Initial wall thickness to cell radius ratio was found using image analysis measurements of cell-section photographs taken with transmission electron microscopy (TEM). A novel mathematical model was developed to correct for random slicing during TEM sample preparation. The effects of osmotic pressure (0-0.8 MPa) and compression rate (1.03-7.68 μm/s) on the bursting force, deformation at bursting, and cell diameter were quantified. Squeeze-hold experiments showed that force relaxation occurred. Bursting force, deformation at bursting, and cell diameter were all significantly affected by osmotic pressure (P <0.05) but were unaffected by compression rate (P ≥ 0.05). Bursting force was found to be correlated with deformation-at-bursting but was independent of cell diameter. These data are analysed in further detail in an accompanying paper (part II). (C) 2000 Elsevier Science Ltd. All rights reserved.",
keywords = "Biological particles, Cell wall, Elasticity, Homogenisation, Micromanipulation, Parameter identification",
author = "Smith, {A. E.} and Z. Zhang and Thomas, {C. R.}",
year = "2000",
month = jun,
doi = "10.1016/S0009-2509(99)00500-X",
language = "English",
volume = "55",
pages = "2031--2041",
journal = "Chemical Engineering Science",
issn = "0009-2509",
publisher = "Elsevier",
number = "11",

}

RIS

TY - JOUR

T1 - Wall material properties of yeast cells

T2 - Part 1. Cell measurements and compression experiments

AU - Smith, A. E.

AU - Zhang, Z.

AU - Thomas, C. R.

PY - 2000/6

Y1 - 2000/6

N2 - To interpret data from the compression experiment (micromanipulation), the initial radial stretch ratio and the wall thickness to cell radius ratio are required. Methods have been developed to measure these parameters for baker's yeast (Saccharomyces cerevisiae) prior to compression experiments. The initial radial stretch ratio was found using osmotic theory to interpret measurements of cell-volume change as a function of external osmotic pressure. Initial wall thickness to cell radius ratio was found using image analysis measurements of cell-section photographs taken with transmission electron microscopy (TEM). A novel mathematical model was developed to correct for random slicing during TEM sample preparation. The effects of osmotic pressure (0-0.8 MPa) and compression rate (1.03-7.68 μm/s) on the bursting force, deformation at bursting, and cell diameter were quantified. Squeeze-hold experiments showed that force relaxation occurred. Bursting force, deformation at bursting, and cell diameter were all significantly affected by osmotic pressure (P <0.05) but were unaffected by compression rate (P ≥ 0.05). Bursting force was found to be correlated with deformation-at-bursting but was independent of cell diameter. These data are analysed in further detail in an accompanying paper (part II). (C) 2000 Elsevier Science Ltd. All rights reserved.

AB - To interpret data from the compression experiment (micromanipulation), the initial radial stretch ratio and the wall thickness to cell radius ratio are required. Methods have been developed to measure these parameters for baker's yeast (Saccharomyces cerevisiae) prior to compression experiments. The initial radial stretch ratio was found using osmotic theory to interpret measurements of cell-volume change as a function of external osmotic pressure. Initial wall thickness to cell radius ratio was found using image analysis measurements of cell-section photographs taken with transmission electron microscopy (TEM). A novel mathematical model was developed to correct for random slicing during TEM sample preparation. The effects of osmotic pressure (0-0.8 MPa) and compression rate (1.03-7.68 μm/s) on the bursting force, deformation at bursting, and cell diameter were quantified. Squeeze-hold experiments showed that force relaxation occurred. Bursting force, deformation at bursting, and cell diameter were all significantly affected by osmotic pressure (P <0.05) but were unaffected by compression rate (P ≥ 0.05). Bursting force was found to be correlated with deformation-at-bursting but was independent of cell diameter. These data are analysed in further detail in an accompanying paper (part II). (C) 2000 Elsevier Science Ltd. All rights reserved.

KW - Biological particles

KW - Cell wall

KW - Elasticity

KW - Homogenisation

KW - Micromanipulation

KW - Parameter identification

UR - http://www.scopus.com/inward/record.url?scp=0034030397&partnerID=8YFLogxK

U2 - 10.1016/S0009-2509(99)00500-X

DO - 10.1016/S0009-2509(99)00500-X

M3 - Article

AN - SCOPUS:0034030397

VL - 55

SP - 2031

EP - 2041

JO - Chemical Engineering Science

JF - Chemical Engineering Science

SN - 0009-2509

IS - 11

ER -