Vitamin D supplementation and antibacterial immune responses in adolescents and young adults with HIV/AIDS

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Vitamin D supplementation and antibacterial immune responses in adolescents and young adults with HIV/AIDS. / Chun, Rene F; Liu, Nancy Q; Lee, T; Schall, Joan I; Denburg, Michelle R; Rutstein, Richard M; Zemel, Babette S; Stallings, Virginia A; Hewison, Martin; Adams, John.

In: The Journal of Steroid Biochemistry and Molecular Biology, Vol. 148, 04.2015, p. 290-7.

Research output: Contribution to journalArticlepeer-review

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APA

Chun, R. F., Liu, N. Q., Lee, T., Schall, J. I., Denburg, M. R., Rutstein, R. M., Zemel, B. S., Stallings, V. A., Hewison, M., & Adams, J. (2015). Vitamin D supplementation and antibacterial immune responses in adolescents and young adults with HIV/AIDS. The Journal of Steroid Biochemistry and Molecular Biology, 148, 290-7. https://doi.org/10.1016/j.jsbmb.2014.07.013

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Chun, Rene F ; Liu, Nancy Q ; Lee, T ; Schall, Joan I ; Denburg, Michelle R ; Rutstein, Richard M ; Zemel, Babette S ; Stallings, Virginia A ; Hewison, Martin ; Adams, John. / Vitamin D supplementation and antibacterial immune responses in adolescents and young adults with HIV/AIDS. In: The Journal of Steroid Biochemistry and Molecular Biology. 2015 ; Vol. 148. pp. 290-7.

Bibtex

@article{9fe30fdce2db4ee693132feb740ca426,
title = "Vitamin D supplementation and antibacterial immune responses in adolescents and young adults with HIV/AIDS",
abstract = "Human monocytes activated by toll-like receptor 2/1 ligand (TLR2/1L) show enhanced expression of the vitamin D receptor (VDR) and the vitamin D-activating enzyme 1α-hydroxylase (CYP27B1). The resulting intracrine conversion of precursor 25-hydroxyvitamin D3 (25OHD) to active 1,25-dihydroxyvitamin D (1,25(OH)2D) can stimulate expression of antibacterial cathelicidin (CAMP). To determine whether this response is functional in HIV-infected subjects (HIV+ ), serum from HIV+ subjects pre- and post-vitamin D supplementation was utilized in monocyte cultures with or without TLR2/1L. Expression of CYP27B1 and VDR was enhanced following treatment with TLR2/1L, although this effect was lower in HIV+ vs HIV- serum (p<0.05). CAMP was also lower in TLR2/1L-treated monocytes cultured in HIV+ serum (p<0.01). In a dose study, supplementation of HIV+ subjects with 4000IU or 7000IU vitamin D/day increased serum 25OHD from 17.3±8.0 and 20.6±6.2ng/ml (43nM and 51nM) at baseline to 41.1±12.0 and 51.9±23.1ng/ml (103nM and 130nM) after 12 weeks (both p<0.001). Greater percent change from baseline 25OHD was significantly associated with enhanced TLR2/1L-induced monocyte CAMP adjusted for baseline expression (p=0.009). In a randomized placebo-controlled trial, 7000IU vitamin D/day increased serum 25OHD from 18.0±8.6 to 32.7±13.8ng/ml (45nM and 82nM) after 12 weeks. Expression of CAMP increased significantly from baseline after 52 weeks of vitamin D-supplementation. At this time point, TLR2/1L-induced CAMP was positively associated with percent change from baseline in 25OHD (p=0.029 overall and 0.002 within vitamin D-supplemented only). These data indicate that vitamin D supplementation in HIV-infected subjects can promote improved antibacterial immunity, but also suggest that longer periods of supplementation are required to achieve this.",
keywords = "Adolescent, Adult, Dietary Supplements, HIV Infections, HIV-1, Humans, Immunity, Innate, Vitamin D, Vitamins, Young Adult",
author = "Chun, {Rene F} and Liu, {Nancy Q} and T Lee and Schall, {Joan I} and Denburg, {Michelle R} and Rutstein, {Richard M} and Zemel, {Babette S} and Stallings, {Virginia A} and Martin Hewison and John Adams",
note = "Copyright {\textcopyright} 2014 Elsevier Ltd. All rights reserved.",
year = "2015",
month = apr,
doi = "10.1016/j.jsbmb.2014.07.013",
language = "English",
volume = "148",
pages = "290--7",
journal = "The Journal of Steroid Biochemistry and Molecular Biology",
issn = "0960-0760",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Vitamin D supplementation and antibacterial immune responses in adolescents and young adults with HIV/AIDS

AU - Chun, Rene F

AU - Liu, Nancy Q

AU - Lee, T

AU - Schall, Joan I

AU - Denburg, Michelle R

AU - Rutstein, Richard M

AU - Zemel, Babette S

AU - Stallings, Virginia A

AU - Hewison, Martin

AU - Adams, John

N1 - Copyright © 2014 Elsevier Ltd. All rights reserved.

PY - 2015/4

Y1 - 2015/4

N2 - Human monocytes activated by toll-like receptor 2/1 ligand (TLR2/1L) show enhanced expression of the vitamin D receptor (VDR) and the vitamin D-activating enzyme 1α-hydroxylase (CYP27B1). The resulting intracrine conversion of precursor 25-hydroxyvitamin D3 (25OHD) to active 1,25-dihydroxyvitamin D (1,25(OH)2D) can stimulate expression of antibacterial cathelicidin (CAMP). To determine whether this response is functional in HIV-infected subjects (HIV+ ), serum from HIV+ subjects pre- and post-vitamin D supplementation was utilized in monocyte cultures with or without TLR2/1L. Expression of CYP27B1 and VDR was enhanced following treatment with TLR2/1L, although this effect was lower in HIV+ vs HIV- serum (p<0.05). CAMP was also lower in TLR2/1L-treated monocytes cultured in HIV+ serum (p<0.01). In a dose study, supplementation of HIV+ subjects with 4000IU or 7000IU vitamin D/day increased serum 25OHD from 17.3±8.0 and 20.6±6.2ng/ml (43nM and 51nM) at baseline to 41.1±12.0 and 51.9±23.1ng/ml (103nM and 130nM) after 12 weeks (both p<0.001). Greater percent change from baseline 25OHD was significantly associated with enhanced TLR2/1L-induced monocyte CAMP adjusted for baseline expression (p=0.009). In a randomized placebo-controlled trial, 7000IU vitamin D/day increased serum 25OHD from 18.0±8.6 to 32.7±13.8ng/ml (45nM and 82nM) after 12 weeks. Expression of CAMP increased significantly from baseline after 52 weeks of vitamin D-supplementation. At this time point, TLR2/1L-induced CAMP was positively associated with percent change from baseline in 25OHD (p=0.029 overall and 0.002 within vitamin D-supplemented only). These data indicate that vitamin D supplementation in HIV-infected subjects can promote improved antibacterial immunity, but also suggest that longer periods of supplementation are required to achieve this.

AB - Human monocytes activated by toll-like receptor 2/1 ligand (TLR2/1L) show enhanced expression of the vitamin D receptor (VDR) and the vitamin D-activating enzyme 1α-hydroxylase (CYP27B1). The resulting intracrine conversion of precursor 25-hydroxyvitamin D3 (25OHD) to active 1,25-dihydroxyvitamin D (1,25(OH)2D) can stimulate expression of antibacterial cathelicidin (CAMP). To determine whether this response is functional in HIV-infected subjects (HIV+ ), serum from HIV+ subjects pre- and post-vitamin D supplementation was utilized in monocyte cultures with or without TLR2/1L. Expression of CYP27B1 and VDR was enhanced following treatment with TLR2/1L, although this effect was lower in HIV+ vs HIV- serum (p<0.05). CAMP was also lower in TLR2/1L-treated monocytes cultured in HIV+ serum (p<0.01). In a dose study, supplementation of HIV+ subjects with 4000IU or 7000IU vitamin D/day increased serum 25OHD from 17.3±8.0 and 20.6±6.2ng/ml (43nM and 51nM) at baseline to 41.1±12.0 and 51.9±23.1ng/ml (103nM and 130nM) after 12 weeks (both p<0.001). Greater percent change from baseline 25OHD was significantly associated with enhanced TLR2/1L-induced monocyte CAMP adjusted for baseline expression (p=0.009). In a randomized placebo-controlled trial, 7000IU vitamin D/day increased serum 25OHD from 18.0±8.6 to 32.7±13.8ng/ml (45nM and 82nM) after 12 weeks. Expression of CAMP increased significantly from baseline after 52 weeks of vitamin D-supplementation. At this time point, TLR2/1L-induced CAMP was positively associated with percent change from baseline in 25OHD (p=0.029 overall and 0.002 within vitamin D-supplemented only). These data indicate that vitamin D supplementation in HIV-infected subjects can promote improved antibacterial immunity, but also suggest that longer periods of supplementation are required to achieve this.

KW - Adolescent

KW - Adult

KW - Dietary Supplements

KW - HIV Infections

KW - HIV-1

KW - Humans

KW - Immunity, Innate

KW - Vitamin D

KW - Vitamins

KW - Young Adult

U2 - 10.1016/j.jsbmb.2014.07.013

DO - 10.1016/j.jsbmb.2014.07.013

M3 - Article

C2 - 25092518

VL - 148

SP - 290

EP - 297

JO - The Journal of Steroid Biochemistry and Molecular Biology

JF - The Journal of Steroid Biochemistry and Molecular Biology

SN - 0960-0760

ER -