Visualization of the joining of ribosomal subunits reveals the presence of 80S ribosomes in the nucleus

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Visualization of the joining of ribosomal subunits reveals the presence of 80S ribosomes in the nucleus. / Al-jubran, K.; Wen, J.; Abdullahi, A.; Roy Choudhury, Subhendu; Li, M.; Ramanathan, P.; Matina, A.; De, S.; Piechocki, K.; Rugjee, K. N.; Brogna, S.

In: RNA, Vol. 19, No. 12, 12.2013, p. 1669-1683.

Research output: Contribution to journalArticle

Harvard

Al-jubran, K, Wen, J, Abdullahi, A, Roy Choudhury, S, Li, M, Ramanathan, P, Matina, A, De, S, Piechocki, K, Rugjee, KN & Brogna, S 2013, 'Visualization of the joining of ribosomal subunits reveals the presence of 80S ribosomes in the nucleus', RNA, vol. 19, no. 12, pp. 1669-1683. https://doi.org/10.1261/rna.038356.113

APA

Al-jubran, K., Wen, J., Abdullahi, A., Roy Choudhury, S., Li, M., Ramanathan, P., Matina, A., De, S., Piechocki, K., Rugjee, K. N., & Brogna, S. (2013). Visualization of the joining of ribosomal subunits reveals the presence of 80S ribosomes in the nucleus. RNA, 19(12), 1669-1683. https://doi.org/10.1261/rna.038356.113

Vancouver

Author

Al-jubran, K. ; Wen, J. ; Abdullahi, A. ; Roy Choudhury, Subhendu ; Li, M. ; Ramanathan, P. ; Matina, A. ; De, S. ; Piechocki, K. ; Rugjee, K. N. ; Brogna, S. / Visualization of the joining of ribosomal subunits reveals the presence of 80S ribosomes in the nucleus. In: RNA. 2013 ; Vol. 19, No. 12. pp. 1669-1683.

Bibtex

@article{0d772837aec74554b7d4825f6b29c238,
title = "Visualization of the joining of ribosomal subunits reveals the presence of 80S ribosomes in the nucleus",
abstract = "In eukaryotes the 40S and 60S ribosomal subunits are assembled in the nucleolus, but there appear to be mechanisms preventing mRNA binding, 80S formation, and initiation of translation in the nucleus. To visualize association between ribosomal subunits, we tagged pairs of Drosophila ribosomal proteins (RPs) located in different subunits with mutually complementing halves of fluorescent proteins. Pairs of tagged RPs expected to interact, or be adjacent in the 80S structure, showed strong fluorescence, while pairs that were not in close proximity did not. Moreover, the complementation signal is found in ribosomal fractions and it was enhanced by translation elongation inhibitors and reduced by initiation inhibitors. Our technique achieved 80S visualization both in cultured cells and in fly tissues in vivo. Notably, while the main 80S signal was in the cytoplasm, clear signals were also seen in the nucleolus and at other nuclear sites. Furthermore, we detected rapid puromycin incorporation in the nucleolus and at transcription sites, providing an independent indication of functional 80S in the nucleolus and 80S association with nascent transcripts.",
keywords = "nuclear ribosomal subunits, ribosomal subunits joining, nascent transcripts, translation",
author = "K. Al-jubran and J. Wen and A. Abdullahi and {Roy Choudhury}, Subhendu and M. Li and P. Ramanathan and A. Matina and S. De and K. Piechocki and Rugjee, {K. N.} and S. Brogna",
year = "2013",
month = dec,
doi = "10.1261/rna.038356.113",
language = "English",
volume = "19",
pages = "1669--1683",
journal = "RNA",
issn = "1355-8382",
publisher = "Cold Spring Harbor Laboratory Press",
number = "12",

}

RIS

TY - JOUR

T1 - Visualization of the joining of ribosomal subunits reveals the presence of 80S ribosomes in the nucleus

AU - Al-jubran, K.

AU - Wen, J.

AU - Abdullahi, A.

AU - Roy Choudhury, Subhendu

AU - Li, M.

AU - Ramanathan, P.

AU - Matina, A.

AU - De, S.

AU - Piechocki, K.

AU - Rugjee, K. N.

AU - Brogna, S.

PY - 2013/12

Y1 - 2013/12

N2 - In eukaryotes the 40S and 60S ribosomal subunits are assembled in the nucleolus, but there appear to be mechanisms preventing mRNA binding, 80S formation, and initiation of translation in the nucleus. To visualize association between ribosomal subunits, we tagged pairs of Drosophila ribosomal proteins (RPs) located in different subunits with mutually complementing halves of fluorescent proteins. Pairs of tagged RPs expected to interact, or be adjacent in the 80S structure, showed strong fluorescence, while pairs that were not in close proximity did not. Moreover, the complementation signal is found in ribosomal fractions and it was enhanced by translation elongation inhibitors and reduced by initiation inhibitors. Our technique achieved 80S visualization both in cultured cells and in fly tissues in vivo. Notably, while the main 80S signal was in the cytoplasm, clear signals were also seen in the nucleolus and at other nuclear sites. Furthermore, we detected rapid puromycin incorporation in the nucleolus and at transcription sites, providing an independent indication of functional 80S in the nucleolus and 80S association with nascent transcripts.

AB - In eukaryotes the 40S and 60S ribosomal subunits are assembled in the nucleolus, but there appear to be mechanisms preventing mRNA binding, 80S formation, and initiation of translation in the nucleus. To visualize association between ribosomal subunits, we tagged pairs of Drosophila ribosomal proteins (RPs) located in different subunits with mutually complementing halves of fluorescent proteins. Pairs of tagged RPs expected to interact, or be adjacent in the 80S structure, showed strong fluorescence, while pairs that were not in close proximity did not. Moreover, the complementation signal is found in ribosomal fractions and it was enhanced by translation elongation inhibitors and reduced by initiation inhibitors. Our technique achieved 80S visualization both in cultured cells and in fly tissues in vivo. Notably, while the main 80S signal was in the cytoplasm, clear signals were also seen in the nucleolus and at other nuclear sites. Furthermore, we detected rapid puromycin incorporation in the nucleolus and at transcription sites, providing an independent indication of functional 80S in the nucleolus and 80S association with nascent transcripts.

KW - nuclear ribosomal subunits

KW - ribosomal subunits joining

KW - nascent transcripts

KW - translation

U2 - 10.1261/rna.038356.113

DO - 10.1261/rna.038356.113

M3 - Article

C2 - 24129492

VL - 19

SP - 1669

EP - 1683

JO - RNA

JF - RNA

SN - 1355-8382

IS - 12

ER -