Vibrational Analysis during Cell Injection in ICSI Operation

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Colleges, School and Institutes

External organisations

  • Department of Mechanical Engineering
  • Birmingham University

Abstract

Intracytoplasmic sperm injection is an assisted reproductive technology in which a single spermatozoon is physically injected into the cytoplasm of an oocyte using a narrow glass needle. Over the past decade, rapid development of intracytoplasmic sperm injection (ICSI) has led to high demand on having precise injector motion and control during the injection task. Injector speed is a restricted dynamical factor in the injection task as it has a direct impact on the amplitude of the lateral displacement at the micropipette tip and therefore cell deformation creation. Analytical and numerical approaches have been instigated and the results have been compared to show the effect of injection speed on lateral displacement of the micro pipette tip. Euler-Bernoulli thin beam theory and the Matlab-Simscape Multibody dynamics simulation tool have been employed for analytical and numerical solution respectively. Cell deformation has been reported when evaluating the resultant lateral displacement of the micropipette during injection. It is found that this displacement helps to pierce the cell membrane during injection. This is due to additional stress created on the cell membrane. An optimal injection speed has thus been determined considering the lateral vibration induced by injection speed and cell deformation.

Details

Original languageEnglish
Title of host publicationMARSS 2018 - International Conference on Manipulation, Automation and Robotics at Small Scales
EditorsSergej Fatikow, Sinan Haliyo, Fumihito Arai, Albert Sill
Publication statusPublished - 3 Oct 2018
Event3rd International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2018 - Nagoya, Japan
Duration: 4 Jul 20188 Jul 2018

Publication series

NameMARSS 2018 - International Conference on Manipulation, Automation and Robotics at Small Scales

Conference

Conference3rd International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2018
CountryJapan
CityNagoya
Period4/07/188/07/18

Keywords

  • cell deformation, ICSI, micro-injection, vibrational analysis