Variable responses of small and large human hepatocytes to hypoxia and hypoxia/reoxygenation (H-R).

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@article{06272f5e05b54a6ca10d9b5b9eeb1dc1,
title = "Variable responses of small and large human hepatocytes to hypoxia and hypoxia/reoxygenation (H-R).",
abstract = "Hypoxia and hypoxia-reoxygenation (H-R) regulate human hepatocyte cell death by mediating the accumulation of reactive oxygen species (ROS). Hepatocytes within the liver are organised into peri-portal (PP) and peri-venous (PV) subpopulations. PP and PV hepatocytes differ in size and function. We investigated whether PP and PV human hepatocytes exhibit differential susceptibility to hypoxic stress. Isolated hepatocytes were used in an in vitro model of hypoxia and H-R. ROS production and cell death were assessed using flow cytometry. PV, and not PP hepatocytes, accumulate intracellular ROS in a mitochondrial dependent manner during hypoxia and H-R. This increased ROS regulates hepatocyte apoptosis and necrosis via a mitochondrial pathway. These findings have implications on the understanding of liver injury and application of potential therapeutic strategies.",
keywords = " Human hepatocytes; Reactive oxygen species; Apoptosis; Necrosis; Hypoxia; Liver injury",
author = "Ricky Bhogal and Christopher Weston and Stuart Curbishley and Anand Bhatt and David Adams and Simon Afford",
year = "2011",
month = mar,
day = "23",
doi = "10.1016/j.febslet.2011.02.030",
language = "English",
volume = "585",
pages = "935--941",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "6",

}

RIS

TY - JOUR

T1 - Variable responses of small and large human hepatocytes to hypoxia and hypoxia/reoxygenation (H-R).

AU - Bhogal, Ricky

AU - Weston, Christopher

AU - Curbishley, Stuart

AU - Bhatt, Anand

AU - Adams, David

AU - Afford, Simon

PY - 2011/3/23

Y1 - 2011/3/23

N2 - Hypoxia and hypoxia-reoxygenation (H-R) regulate human hepatocyte cell death by mediating the accumulation of reactive oxygen species (ROS). Hepatocytes within the liver are organised into peri-portal (PP) and peri-venous (PV) subpopulations. PP and PV hepatocytes differ in size and function. We investigated whether PP and PV human hepatocytes exhibit differential susceptibility to hypoxic stress. Isolated hepatocytes were used in an in vitro model of hypoxia and H-R. ROS production and cell death were assessed using flow cytometry. PV, and not PP hepatocytes, accumulate intracellular ROS in a mitochondrial dependent manner during hypoxia and H-R. This increased ROS regulates hepatocyte apoptosis and necrosis via a mitochondrial pathway. These findings have implications on the understanding of liver injury and application of potential therapeutic strategies.

AB - Hypoxia and hypoxia-reoxygenation (H-R) regulate human hepatocyte cell death by mediating the accumulation of reactive oxygen species (ROS). Hepatocytes within the liver are organised into peri-portal (PP) and peri-venous (PV) subpopulations. PP and PV hepatocytes differ in size and function. We investigated whether PP and PV human hepatocytes exhibit differential susceptibility to hypoxic stress. Isolated hepatocytes were used in an in vitro model of hypoxia and H-R. ROS production and cell death were assessed using flow cytometry. PV, and not PP hepatocytes, accumulate intracellular ROS in a mitochondrial dependent manner during hypoxia and H-R. This increased ROS regulates hepatocyte apoptosis and necrosis via a mitochondrial pathway. These findings have implications on the understanding of liver injury and application of potential therapeutic strategies.

KW - Human hepatocytes; Reactive oxygen species; Apoptosis; Necrosis; Hypoxia; Liver injury

U2 - 10.1016/j.febslet.2011.02.030

DO - 10.1016/j.febslet.2011.02.030

M3 - Article

C2 - 21356211

VL - 585

SP - 935

EP - 941

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 6

ER -