Using Flow Cytometry to Analyze Cryptococcus Infection of Macrophages

Research output: Contribution to journalArticlepeer-review

Standard

Using Flow Cytometry to Analyze Cryptococcus Infection of Macrophages. / Evans, Robert; Voelz, Kerstin; Johnston, Simon; May, Robin.

In: Methods in molecular biology, Vol. 1519, 05.11.2016, p. 349-357.

Research output: Contribution to journalArticlepeer-review

Harvard

APA

Vancouver

Author

Bibtex

@article{618048ce929047bb9050bff7066de534,
title = "Using Flow Cytometry to Analyze Cryptococcus Infection of Macrophages",
abstract = "Flow cytometry is a powerful analytical technique, which is increasingly being used to study the interaction between host cells and intracellular pathogens. Flow cytometry is capable of measuring a greater number of infected cells within a sample compared to alternative techniques such as fluorescence microscopy. This means that robust quantification of rare events during infection is possible. Our lab and others have developed flow cytometry methods to study interactions between host cells and intracellular pathogens, such as Cryptococcus neoformans, to quantify phagocytosis, intracellular replication, and non-lytic expulsion or “vomocytosis” from the phagosome. Herein we describe these methods and how they can be applied to the study of C. neoformans as well as other similar intracellular pathogens.",
author = "Robert Evans and Kerstin Voelz and Simon Johnston and Robin May",
year = "2016",
month = nov,
day = "5",
doi = "10.1007/978-1-4939-6581-6_24",
language = "English",
volume = "1519",
pages = "349--357",
journal = "Methods in molecular biology",
issn = "1064-3745",
publisher = "Springer",

}

RIS

TY - JOUR

T1 - Using Flow Cytometry to Analyze Cryptococcus Infection of Macrophages

AU - Evans, Robert

AU - Voelz, Kerstin

AU - Johnston, Simon

AU - May, Robin

PY - 2016/11/5

Y1 - 2016/11/5

N2 - Flow cytometry is a powerful analytical technique, which is increasingly being used to study the interaction between host cells and intracellular pathogens. Flow cytometry is capable of measuring a greater number of infected cells within a sample compared to alternative techniques such as fluorescence microscopy. This means that robust quantification of rare events during infection is possible. Our lab and others have developed flow cytometry methods to study interactions between host cells and intracellular pathogens, such as Cryptococcus neoformans, to quantify phagocytosis, intracellular replication, and non-lytic expulsion or “vomocytosis” from the phagosome. Herein we describe these methods and how they can be applied to the study of C. neoformans as well as other similar intracellular pathogens.

AB - Flow cytometry is a powerful analytical technique, which is increasingly being used to study the interaction between host cells and intracellular pathogens. Flow cytometry is capable of measuring a greater number of infected cells within a sample compared to alternative techniques such as fluorescence microscopy. This means that robust quantification of rare events during infection is possible. Our lab and others have developed flow cytometry methods to study interactions between host cells and intracellular pathogens, such as Cryptococcus neoformans, to quantify phagocytosis, intracellular replication, and non-lytic expulsion or “vomocytosis” from the phagosome. Herein we describe these methods and how they can be applied to the study of C. neoformans as well as other similar intracellular pathogens.

U2 - 10.1007/978-1-4939-6581-6_24

DO - 10.1007/978-1-4939-6581-6_24

M3 - Article

C2 - 27815892

VL - 1519

SP - 349

EP - 357

JO - Methods in molecular biology

JF - Methods in molecular biology

SN - 1064-3745

ER -