Using Ex Vivo Liver Organ Cultures to Measure Lymphocyte Trafficking

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Authors

Colleges, School and Institutes

External organisations

  • Centre for Liver Research, Immunity and Immunotherapy, Institute of Biomedical Research, University of Birmingham, Birmingham, B15 2TT, UK.

Abstract

Lymphocyte recruitment to different organs, and even alternate anatomical regions within the same organ, is differentially regulated. Key combinations of adhesion molecules and chemokines govern compartmentalization, and these can change depending upon the nature and duration of tissue injury. We are interested in understanding lymphocyte recruitment to the liver during human disease, and thus need models of the liver inflammatory milieu that are as representative as possible. Here we describe the use of precision cut liver slices as models of disease.

Details

Original languageEnglish
Title of host publicationT-Cell Trafficking:
Subtitle of host publicationMethods and Protocols
EditorsGeorge Edward Rainger, Helen M. Mcgettrick
Publication statusPublished - 28 Mar 2017

Publication series

NameMethods in Molecular Biology
PublisherSpringer
ISSN (Print)1064-3745

Keywords

  • Journal Article, antibody , human , inflammation , liver , lymphocyte , organ culture