Use of REMI and Agrobacterium-mediated transformation to identify pathogenicity mutants of the biocontrol fungus Coniothyrium minitans

Research output: Contribution to journalArticle

Authors

Colleges, School and Institutes

Abstract

Restriction enzyme mediated integration (REMI) and Agrobacterium-mediated transformation (ATMT) were used to transform protoplasts or germinated conidia of the mycoparasite Coniothyrium minitans to hygromycin resistance. Using REMI, up to 32 transformants mug DNA(-1) were obtained, while 37.8 transformants 5 x 10(5) germlings(-1) were obtained using ATMT. Single-copy integrations occurred in 8% and 40% of REMI and ATMT transformants, respectively. A novel microtitre plate-based test was developed to expedite screening of 4000 REMI and ATMT C. minitans transformants. Nine pathogenicity mutants that displayed reduced or no pathogenicity on sclerotia of Sclerotinia sclerotiorum were identified.

Details

Original languageEnglish
Pages (from-to)207-214
Number of pages8
JournalFEMS Microbiology Letters
Volume241
Issue number2
Publication statusPublished - 1 Jan 2004

Keywords

  • Agrobacterium tumefaciens-mediated transformation, pathogenicity, REMI, Coniothyrium minitans, mycoparasitism