Use of environmental scanning electron microscopy to image the spore ahesive of the marine alga Enteromorpha in its natural hydrated state

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Colleges, School and Institutes

Abstract

The environmental scanning electron microscope (ESEM) has been used to image the adhesive secreted by zoospores of the marine alga Enteromorpha as they settle on a surface, under natural, hydrated conditions. Results reveal a featureless, swollen gel-like adhesive pad, in contrast to the fibrillar character of the adhesive when imaged by standard SEM. At high spore densities the adhesive is confluent. Dynamic hydration/dehydration events were followed by changing the water vapour pressure in the sample chamber. Rapid hydration and swelling were observed indicating a very hygroscopic material. Adhesive footprints were detected when surfaces from which spores had been removed by water jetting were examined. (C) 2002 Elsevier Science B.V. All rights reserved.

Details

Original languageEnglish
Pages (from-to)315-321
Number of pages7
JournalColloids and Surfaces B: Biointerfaces
Volume27
Issue number4
Publication statusPublished - 1 Mar 2003

Keywords

  • spores, adhesive, environmental scanning electron microscope (ESEM), marine algae, cell adhesion