Upper aerodigestive tract squamous cell carcinomas show distinct overall DNA methylation profiles and different molecular mechanisms behind WNT signaling disruption

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Upper aerodigestive tract squamous cell carcinomas show distinct overall DNA methylation profiles and different molecular mechanisms behind WNT signaling disruption. / Soares-lima, Sheila Coelho; Mehanna, Hisham; Camuzi, Diego; De Souza-santos, Paulo Thiago; Simão, Tatiana De Almeida; Nicolau-neto, Pedro; Almeida Lopes, Monique De Souza; Cuenin, Cyrille; Talukdar, Fazlur Rahman; Batis, Nikolaos; Costa, Izabella; Dias, Fernando; Degli Esposti, Davide; Boroni, Mariana; Herceg, Zdenko; Ribeiro Pinto, Luis Felipe.

In: Cancers, Vol. 13, No. 12, 3014, 16.06.2021.

Research output: Contribution to journalArticlepeer-review

Harvard

Soares-lima, SC, Mehanna, H, Camuzi, D, De Souza-santos, PT, Simão, TDA, Nicolau-neto, P, Almeida Lopes, MDS, Cuenin, C, Talukdar, FR, Batis, N, Costa, I, Dias, F, Degli Esposti, D, Boroni, M, Herceg, Z & Ribeiro Pinto, LF 2021, 'Upper aerodigestive tract squamous cell carcinomas show distinct overall DNA methylation profiles and different molecular mechanisms behind WNT signaling disruption', Cancers, vol. 13, no. 12, 3014. https://doi.org/10.3390/cancers13123014

APA

Soares-lima, S. C., Mehanna, H., Camuzi, D., De Souza-santos, P. T., Simão, T. D. A., Nicolau-neto, P., Almeida Lopes, M. D. S., Cuenin, C., Talukdar, F. R., Batis, N., Costa, I., Dias, F., Degli Esposti, D., Boroni, M., Herceg, Z., & Ribeiro Pinto, L. F. (2021). Upper aerodigestive tract squamous cell carcinomas show distinct overall DNA methylation profiles and different molecular mechanisms behind WNT signaling disruption. Cancers, 13(12), [3014]. https://doi.org/10.3390/cancers13123014

Vancouver

Author

Soares-lima, Sheila Coelho ; Mehanna, Hisham ; Camuzi, Diego ; De Souza-santos, Paulo Thiago ; Simão, Tatiana De Almeida ; Nicolau-neto, Pedro ; Almeida Lopes, Monique De Souza ; Cuenin, Cyrille ; Talukdar, Fazlur Rahman ; Batis, Nikolaos ; Costa, Izabella ; Dias, Fernando ; Degli Esposti, Davide ; Boroni, Mariana ; Herceg, Zdenko ; Ribeiro Pinto, Luis Felipe. / Upper aerodigestive tract squamous cell carcinomas show distinct overall DNA methylation profiles and different molecular mechanisms behind WNT signaling disruption. In: Cancers. 2021 ; Vol. 13, No. 12.

Bibtex

@article{63275c26ec564a33b31dbf65d2fe21ec,
title = "Upper aerodigestive tract squamous cell carcinomas show distinct overall DNA methylation profiles and different molecular mechanisms behind WNT signaling disruption",
abstract = "Upper aerodigestive tract (UADT) tumors present different biological behavior and prog-38 nosis, suggesting specific molecular mechanisms underlying their development. However, they are 39 rarely considered as single entities (particularly head and neck subsites) and share the most com-40 mon genetic alterations. Therefore, there is a need for a better understanding of the global DNA 41 methylation differences among UADT tumors. We performed a genome-wide DNA methylation 42 analysis of esophageal (ESCC), laryngeal (LSCC), oral (OSCC) and oropharyngeal (OPSCC) squa-43 mous cell carcinomas, and their non-tumor counterparts. The unsupervised analysis showed that 44 non-tumor tissues present markedly distinct DNA methylation profiles, while tumors are highly 45 heterogeneous. Hypomethylation was more frequent in LSCC and OPSCC, while ESCC and OSCC 46 presented mostly hypermethylation, with the latter showing a CpG island overrepresentation. Dif-47 ferentially methylated regions affected genes in 127 signaling pathways, with only 3.1% of these being common among different tumor subsites, but with different genes affected. The WNT signal-49 ing pathway, known to be dysregulated in different epithelial tumors, is a frequent hit for DNA 50 methylation and gene expression alterations in ESCC and OPSCC, but mostly for genetic alterations 51 in LSCC and OSCC. UADT tumor subsites present differences in genome-wide methylation regard-52 ing their profile, intensity, genomic regions and signaling pathways affected.",
author = "Soares-lima, {Sheila Coelho} and Hisham Mehanna and Diego Camuzi and {De Souza-santos}, {Paulo Thiago} and Sim{\~a}o, {Tatiana De Almeida} and Pedro Nicolau-neto and {Almeida Lopes}, {Monique De Souza} and Cyrille Cuenin and Talukdar, {Fazlur Rahman} and Nikolaos Batis and Izabella Costa and Fernando Dias and {Degli Esposti}, Davide and Mariana Boroni and Zdenko Herceg and {Ribeiro Pinto}, {Luis Felipe}",
year = "2021",
month = jun,
day = "16",
doi = "10.3390/cancers13123014",
language = "English",
volume = "13",
journal = "Cancers",
issn = "2072-6694",
publisher = "MDPI",
number = "12",

}

RIS

TY - JOUR

T1 - Upper aerodigestive tract squamous cell carcinomas show distinct overall DNA methylation profiles and different molecular mechanisms behind WNT signaling disruption

AU - Soares-lima, Sheila Coelho

AU - Mehanna, Hisham

AU - Camuzi, Diego

AU - De Souza-santos, Paulo Thiago

AU - Simão, Tatiana De Almeida

AU - Nicolau-neto, Pedro

AU - Almeida Lopes, Monique De Souza

AU - Cuenin, Cyrille

AU - Talukdar, Fazlur Rahman

AU - Batis, Nikolaos

AU - Costa, Izabella

AU - Dias, Fernando

AU - Degli Esposti, Davide

AU - Boroni, Mariana

AU - Herceg, Zdenko

AU - Ribeiro Pinto, Luis Felipe

PY - 2021/6/16

Y1 - 2021/6/16

N2 - Upper aerodigestive tract (UADT) tumors present different biological behavior and prog-38 nosis, suggesting specific molecular mechanisms underlying their development. However, they are 39 rarely considered as single entities (particularly head and neck subsites) and share the most com-40 mon genetic alterations. Therefore, there is a need for a better understanding of the global DNA 41 methylation differences among UADT tumors. We performed a genome-wide DNA methylation 42 analysis of esophageal (ESCC), laryngeal (LSCC), oral (OSCC) and oropharyngeal (OPSCC) squa-43 mous cell carcinomas, and their non-tumor counterparts. The unsupervised analysis showed that 44 non-tumor tissues present markedly distinct DNA methylation profiles, while tumors are highly 45 heterogeneous. Hypomethylation was more frequent in LSCC and OPSCC, while ESCC and OSCC 46 presented mostly hypermethylation, with the latter showing a CpG island overrepresentation. Dif-47 ferentially methylated regions affected genes in 127 signaling pathways, with only 3.1% of these being common among different tumor subsites, but with different genes affected. The WNT signal-49 ing pathway, known to be dysregulated in different epithelial tumors, is a frequent hit for DNA 50 methylation and gene expression alterations in ESCC and OPSCC, but mostly for genetic alterations 51 in LSCC and OSCC. UADT tumor subsites present differences in genome-wide methylation regard-52 ing their profile, intensity, genomic regions and signaling pathways affected.

AB - Upper aerodigestive tract (UADT) tumors present different biological behavior and prog-38 nosis, suggesting specific molecular mechanisms underlying their development. However, they are 39 rarely considered as single entities (particularly head and neck subsites) and share the most com-40 mon genetic alterations. Therefore, there is a need for a better understanding of the global DNA 41 methylation differences among UADT tumors. We performed a genome-wide DNA methylation 42 analysis of esophageal (ESCC), laryngeal (LSCC), oral (OSCC) and oropharyngeal (OPSCC) squa-43 mous cell carcinomas, and their non-tumor counterparts. The unsupervised analysis showed that 44 non-tumor tissues present markedly distinct DNA methylation profiles, while tumors are highly 45 heterogeneous. Hypomethylation was more frequent in LSCC and OPSCC, while ESCC and OSCC 46 presented mostly hypermethylation, with the latter showing a CpG island overrepresentation. Dif-47 ferentially methylated regions affected genes in 127 signaling pathways, with only 3.1% of these being common among different tumor subsites, but with different genes affected. The WNT signal-49 ing pathway, known to be dysregulated in different epithelial tumors, is a frequent hit for DNA 50 methylation and gene expression alterations in ESCC and OPSCC, but mostly for genetic alterations 51 in LSCC and OSCC. UADT tumor subsites present differences in genome-wide methylation regard-52 ing their profile, intensity, genomic regions and signaling pathways affected.

U2 - 10.3390/cancers13123014

DO - 10.3390/cancers13123014

M3 - Article

VL - 13

JO - Cancers

JF - Cancers

SN - 2072-6694

IS - 12

M1 - 3014

ER -