Unexpected patterns of Epstein-Barr virus transcription revealed by a High throughput PCR array for absolute quantification of viral mRNA

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@article{62e8b4ba752a46889019b2a6c2522819,
title = "Unexpected patterns of Epstein-Barr virus transcription revealed by a High throughput PCR array for absolute quantification of viral mRNA",
abstract = "We have validated a flexible, high-throughput and relatively inexpensive RT-QPCR array platform for absolute quantification of Epstein-Barr virus transcripts in different latent and lytic infection states. Several novel observations are reported. First, during infection of normal B cells, Wp-initiated latent gene transcripts remain far more abundant following activation of the Cp promoter than was hitherto suspected. Second, EBNA1 transcript levels are remarkably low in all forms of latency, typically ranging from 1 to 10 transcripts per cell. EBNA3A, -3B and -3C transcripts are likewise very low in Latency III, typically at levels similar to or less than EBNA1 transcripts. Thirdly, a subset of lytic gene transcripts is detectable in Burkitt lymphoma lines at low levels, including: BILF1, which has oncogenic properties, and the poorly characterized LF1, LF2 and LF3 genes. Analysis of seven African BL biopsies confirmed this transcription profile but additionally revealed significant expression of LMP2 transcripts.",
keywords = "Epstein–Barr virus, HHV4, Herpesvirus, Viral gene expression, Transcription, Lytic replication, Latency, Burkitt lymphoma, Tumor virus",
author = "Rosemary Tierney and Shannon-Lowe, {Claire D} and Leah Fitzsimmons and Andrew Bell and Martin Rowe",
year = "2015",
month = jan,
day = "1",
doi = "10.1016/j.virol.2014.10.030",
language = "English",
volume = "474",
pages = "117--30",
journal = "Virology",
issn = "0042-6822",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Unexpected patterns of Epstein-Barr virus transcription revealed by a High throughput PCR array for absolute quantification of viral mRNA

AU - Tierney, Rosemary

AU - Shannon-Lowe, Claire D

AU - Fitzsimmons, Leah

AU - Bell, Andrew

AU - Rowe, Martin

PY - 2015/1/1

Y1 - 2015/1/1

N2 - We have validated a flexible, high-throughput and relatively inexpensive RT-QPCR array platform for absolute quantification of Epstein-Barr virus transcripts in different latent and lytic infection states. Several novel observations are reported. First, during infection of normal B cells, Wp-initiated latent gene transcripts remain far more abundant following activation of the Cp promoter than was hitherto suspected. Second, EBNA1 transcript levels are remarkably low in all forms of latency, typically ranging from 1 to 10 transcripts per cell. EBNA3A, -3B and -3C transcripts are likewise very low in Latency III, typically at levels similar to or less than EBNA1 transcripts. Thirdly, a subset of lytic gene transcripts is detectable in Burkitt lymphoma lines at low levels, including: BILF1, which has oncogenic properties, and the poorly characterized LF1, LF2 and LF3 genes. Analysis of seven African BL biopsies confirmed this transcription profile but additionally revealed significant expression of LMP2 transcripts.

AB - We have validated a flexible, high-throughput and relatively inexpensive RT-QPCR array platform for absolute quantification of Epstein-Barr virus transcripts in different latent and lytic infection states. Several novel observations are reported. First, during infection of normal B cells, Wp-initiated latent gene transcripts remain far more abundant following activation of the Cp promoter than was hitherto suspected. Second, EBNA1 transcript levels are remarkably low in all forms of latency, typically ranging from 1 to 10 transcripts per cell. EBNA3A, -3B and -3C transcripts are likewise very low in Latency III, typically at levels similar to or less than EBNA1 transcripts. Thirdly, a subset of lytic gene transcripts is detectable in Burkitt lymphoma lines at low levels, including: BILF1, which has oncogenic properties, and the poorly characterized LF1, LF2 and LF3 genes. Analysis of seven African BL biopsies confirmed this transcription profile but additionally revealed significant expression of LMP2 transcripts.

KW - Epstein–Barr virus

KW - HHV4

KW - Herpesvirus

KW - Viral gene expression

KW - Transcription

KW - Lytic replication

KW - Latency

KW - Burkitt lymphoma

KW - Tumor virus

U2 - 10.1016/j.virol.2014.10.030

DO - 10.1016/j.virol.2014.10.030

M3 - Article

C2 - 25463610

VL - 474

SP - 117

EP - 130

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1

ER -