Uncoupling VEGFA Functions in Arteriogenesis and Hematopoietic Stem Cell Specification

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Uncoupling VEGFA Functions in Arteriogenesis and Hematopoietic Stem Cell Specification. / Leung, Amy; Ciau-Uitz, Aldo; Pinheiro, Philip; Monteiro, Rui; Zuo, Jie; Vyas, Paresh; Patient, Roger; Porcher, Catherine.

In: Developmental Cell, Vol. 24, No. 2, 28.01.2013, p. 144-158.

Research output: Contribution to journalArticlepeer-review

Harvard

Leung, A, Ciau-Uitz, A, Pinheiro, P, Monteiro, R, Zuo, J, Vyas, P, Patient, R & Porcher, C 2013, 'Uncoupling VEGFA Functions in Arteriogenesis and Hematopoietic Stem Cell Specification', Developmental Cell, vol. 24, no. 2, pp. 144-158. https://doi.org/10.1016/j.devcel.2012.12.004

APA

Leung, A., Ciau-Uitz, A., Pinheiro, P., Monteiro, R., Zuo, J., Vyas, P., Patient, R., & Porcher, C. (2013). Uncoupling VEGFA Functions in Arteriogenesis and Hematopoietic Stem Cell Specification. Developmental Cell, 24(2), 144-158. https://doi.org/10.1016/j.devcel.2012.12.004

Vancouver

Author

Leung, Amy ; Ciau-Uitz, Aldo ; Pinheiro, Philip ; Monteiro, Rui ; Zuo, Jie ; Vyas, Paresh ; Patient, Roger ; Porcher, Catherine. / Uncoupling VEGFA Functions in Arteriogenesis and Hematopoietic Stem Cell Specification. In: Developmental Cell. 2013 ; Vol. 24, No. 2. pp. 144-158.

Bibtex

@article{afa5f087bf0e4503a5ebac7a7d844285,
title = "Uncoupling VEGFA Functions in Arteriogenesis and Hematopoietic Stem Cell Specification",
abstract = "VEGFA signaling is critical for endothelial and hematopoietic stem cell (HSC) specification. However, blood defects resulting from perturbation of the VEGFA pathway are always accompanied by impaired vascular/arterial development. Because HSCs derive from arterial cells, it is unclear whether VEGFA directly contributes to HSC specification. This is an important question for our understanding of how HSCs are formed and for developing their production in vitro. Through knockdown of the regulator ETO2 in embryogenesis, we report a specific decrease in expression of medium/long Vegfa isoforms in somites. This leads to absence of Notch1 expression and failure of HSC specification in the dorsal aorta (DA), independently of vessel formation and arterial specification. Vegfa hypomorphs and isoform-specific (medium/long) morphants not only recapitulate this phenotype but also demonstrate that VEGFA short isoform is sufficient for DA development. Therefore, sequential, isoform-specific VEGFA signaling successively induces the endothelial, arterial, and HSC programs in the DA.",
author = "Amy Leung and Aldo Ciau-Uitz and Philip Pinheiro and Rui Monteiro and Jie Zuo and Paresh Vyas and Roger Patient and Catherine Porcher",
year = "2013",
month = jan,
day = "28",
doi = "10.1016/j.devcel.2012.12.004",
language = "English",
volume = "24",
pages = "144--158",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Uncoupling VEGFA Functions in Arteriogenesis and Hematopoietic Stem Cell Specification

AU - Leung, Amy

AU - Ciau-Uitz, Aldo

AU - Pinheiro, Philip

AU - Monteiro, Rui

AU - Zuo, Jie

AU - Vyas, Paresh

AU - Patient, Roger

AU - Porcher, Catherine

PY - 2013/1/28

Y1 - 2013/1/28

N2 - VEGFA signaling is critical for endothelial and hematopoietic stem cell (HSC) specification. However, blood defects resulting from perturbation of the VEGFA pathway are always accompanied by impaired vascular/arterial development. Because HSCs derive from arterial cells, it is unclear whether VEGFA directly contributes to HSC specification. This is an important question for our understanding of how HSCs are formed and for developing their production in vitro. Through knockdown of the regulator ETO2 in embryogenesis, we report a specific decrease in expression of medium/long Vegfa isoforms in somites. This leads to absence of Notch1 expression and failure of HSC specification in the dorsal aorta (DA), independently of vessel formation and arterial specification. Vegfa hypomorphs and isoform-specific (medium/long) morphants not only recapitulate this phenotype but also demonstrate that VEGFA short isoform is sufficient for DA development. Therefore, sequential, isoform-specific VEGFA signaling successively induces the endothelial, arterial, and HSC programs in the DA.

AB - VEGFA signaling is critical for endothelial and hematopoietic stem cell (HSC) specification. However, blood defects resulting from perturbation of the VEGFA pathway are always accompanied by impaired vascular/arterial development. Because HSCs derive from arterial cells, it is unclear whether VEGFA directly contributes to HSC specification. This is an important question for our understanding of how HSCs are formed and for developing their production in vitro. Through knockdown of the regulator ETO2 in embryogenesis, we report a specific decrease in expression of medium/long Vegfa isoforms in somites. This leads to absence of Notch1 expression and failure of HSC specification in the dorsal aorta (DA), independently of vessel formation and arterial specification. Vegfa hypomorphs and isoform-specific (medium/long) morphants not only recapitulate this phenotype but also demonstrate that VEGFA short isoform is sufficient for DA development. Therefore, sequential, isoform-specific VEGFA signaling successively induces the endothelial, arterial, and HSC programs in the DA.

UR - http://www.scopus.com/inward/record.url?scp=84873094555&partnerID=8YFLogxK

U2 - 10.1016/j.devcel.2012.12.004

DO - 10.1016/j.devcel.2012.12.004

M3 - Article

C2 - 23318133

AN - SCOPUS:84873094555

VL - 24

SP - 144

EP - 158

JO - Developmental Cell

JF - Developmental Cell

SN - 1534-5807

IS - 2

ER -