Uncoupled turnover disrupts mitochondrial quality control in diabetic retinopathy

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Uncoupled turnover disrupts mitochondrial quality control in diabetic retinopathy. / Romero, Jose; Cairns, Lauren; Dutton, Louise; Lyons, Timothy; Brazil, Derek; Moynagh, Paul; Curtis, Tim; Xu, Heping.

In: JCI Insight, Vol. 4, No. 23, e129760, 05.12.2019.

Research output: Contribution to journalArticlepeer-review

Harvard

Romero, J, Cairns, L, Dutton, L, Lyons, T, Brazil, D, Moynagh, P, Curtis, T & Xu, H 2019, 'Uncoupled turnover disrupts mitochondrial quality control in diabetic retinopathy', JCI Insight, vol. 4, no. 23, e129760. https://doi.org/10.1172/jci.insight.129760

APA

Romero, J., Cairns, L., Dutton, L., Lyons, T., Brazil, D., Moynagh, P., Curtis, T., & Xu, H. (2019). Uncoupled turnover disrupts mitochondrial quality control in diabetic retinopathy. JCI Insight, 4(23), [e129760]. https://doi.org/10.1172/jci.insight.129760

Vancouver

Author

Romero, Jose ; Cairns, Lauren ; Dutton, Louise ; Lyons, Timothy ; Brazil, Derek ; Moynagh, Paul ; Curtis, Tim ; Xu, Heping. / Uncoupled turnover disrupts mitochondrial quality control in diabetic retinopathy. In: JCI Insight. 2019 ; Vol. 4, No. 23.

Bibtex

@article{a28cab975ad94dfc804190bf6c832376,
title = "Uncoupled turnover disrupts mitochondrial quality control in diabetic retinopathy",
abstract = "Mitochondrial quality control (MQC) is crucial for regulating CNS homeostasis, and its disruption has been implicated in the pathogenesis of some of the most common neurodegenerative diseases. In healthy tissues, the maintenance of MQC depends upon an exquisite balance between mitophagy (removal of damaged mitochondria by autophagy) and biogenesis (de novo synthesis of mitochondria). Here, we show that mitophagy is disrupted in diabetic retinopathy (DR) and decoupled from mitochondrial biogenesis during the progression of the disease. Diabetic retinas from human postmortem donors and experimental mice exhibit a net loss of mitochondrial contents during the early stages of the disease process. Using diabetic mitophagy-reporter mice (mitoQC-Ins2Akita) alongside pMitoTimer (a molecular clock to address mitochondrial age dynamics), we demonstrate that mitochondrial loss arose due to an inability of mitochondrial biogenesis to compensate for diabetes-exacerbated mitophagy. However, as diabetes duration increases, Pink1-dependent mitophagy deteriorates, leading to the build-up of mitochondria primed for degradation in DR. Impairment of mitophagy during prolonged diabetes is linked with the development of retinal senescence, a phenotype that blunted hyperglycemia-induced mitophagy in mitoQC primary M{\"u}ller cells. Our findings suggest that normalizing mitochondrial turnover may preserve MQC and provide therapeutic options for the management of DR-associated complications.",
keywords = "Autophagy, Mitochondria, Neuroscience, Ophthalmology, Retinopathy",
author = "Jose Romero and Lauren Cairns and Louise Dutton and Timothy Lyons and Derek Brazil and Paul Moynagh and Tim Curtis and Heping Xu",
year = "2019",
month = dec,
day = "5",
doi = "10.1172/jci.insight.129760",
language = "English",
volume = "4",
journal = "JCI Insight",
issn = "2379-3708",
publisher = "American Society for Clinical Investigation",
number = "23",

}

RIS

TY - JOUR

T1 - Uncoupled turnover disrupts mitochondrial quality control in diabetic retinopathy

AU - Romero, Jose

AU - Cairns, Lauren

AU - Dutton, Louise

AU - Lyons, Timothy

AU - Brazil, Derek

AU - Moynagh, Paul

AU - Curtis, Tim

AU - Xu, Heping

PY - 2019/12/5

Y1 - 2019/12/5

N2 - Mitochondrial quality control (MQC) is crucial for regulating CNS homeostasis, and its disruption has been implicated in the pathogenesis of some of the most common neurodegenerative diseases. In healthy tissues, the maintenance of MQC depends upon an exquisite balance between mitophagy (removal of damaged mitochondria by autophagy) and biogenesis (de novo synthesis of mitochondria). Here, we show that mitophagy is disrupted in diabetic retinopathy (DR) and decoupled from mitochondrial biogenesis during the progression of the disease. Diabetic retinas from human postmortem donors and experimental mice exhibit a net loss of mitochondrial contents during the early stages of the disease process. Using diabetic mitophagy-reporter mice (mitoQC-Ins2Akita) alongside pMitoTimer (a molecular clock to address mitochondrial age dynamics), we demonstrate that mitochondrial loss arose due to an inability of mitochondrial biogenesis to compensate for diabetes-exacerbated mitophagy. However, as diabetes duration increases, Pink1-dependent mitophagy deteriorates, leading to the build-up of mitochondria primed for degradation in DR. Impairment of mitophagy during prolonged diabetes is linked with the development of retinal senescence, a phenotype that blunted hyperglycemia-induced mitophagy in mitoQC primary Müller cells. Our findings suggest that normalizing mitochondrial turnover may preserve MQC and provide therapeutic options for the management of DR-associated complications.

AB - Mitochondrial quality control (MQC) is crucial for regulating CNS homeostasis, and its disruption has been implicated in the pathogenesis of some of the most common neurodegenerative diseases. In healthy tissues, the maintenance of MQC depends upon an exquisite balance between mitophagy (removal of damaged mitochondria by autophagy) and biogenesis (de novo synthesis of mitochondria). Here, we show that mitophagy is disrupted in diabetic retinopathy (DR) and decoupled from mitochondrial biogenesis during the progression of the disease. Diabetic retinas from human postmortem donors and experimental mice exhibit a net loss of mitochondrial contents during the early stages of the disease process. Using diabetic mitophagy-reporter mice (mitoQC-Ins2Akita) alongside pMitoTimer (a molecular clock to address mitochondrial age dynamics), we demonstrate that mitochondrial loss arose due to an inability of mitochondrial biogenesis to compensate for diabetes-exacerbated mitophagy. However, as diabetes duration increases, Pink1-dependent mitophagy deteriorates, leading to the build-up of mitochondria primed for degradation in DR. Impairment of mitophagy during prolonged diabetes is linked with the development of retinal senescence, a phenotype that blunted hyperglycemia-induced mitophagy in mitoQC primary Müller cells. Our findings suggest that normalizing mitochondrial turnover may preserve MQC and provide therapeutic options for the management of DR-associated complications.

KW - Autophagy

KW - Mitochondria

KW - Neuroscience

KW - Ophthalmology

KW - Retinopathy

UR - https://insight.jci.org/articles/view/129760

U2 - 10.1172/jci.insight.129760

DO - 10.1172/jci.insight.129760

M3 - Article

C2 - 31661466

VL - 4

JO - JCI Insight

JF - JCI Insight

SN - 2379-3708

IS - 23

M1 - e129760

ER -