Unbiased screening of polymer libraries to define novel substrates for functional hepatocytes with inducible drug metabolism

Research output: Contribution to journalArticle

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Unbiased screening of polymer libraries to define novel substrates for functional hepatocytes with inducible drug metabolism. / Hay, DC; Pernagallo, S; Diaz-Mochon, JJ; Medine, CN; Greenhough, S; Hannoun, Z; Schrader, J; Black, JR; Fletcher, J; Dalgetty, D; Thompson, AI; Newsome, Philip; Forbes, SJ; Ross, JA; Bradley, M; Iredale, JP.

In: Stem Cell Research, Vol. 6, No. 2, 01.03.2011, p. 92-102.

Research output: Contribution to journalArticle

Harvard

Hay, DC, Pernagallo, S, Diaz-Mochon, JJ, Medine, CN, Greenhough, S, Hannoun, Z, Schrader, J, Black, JR, Fletcher, J, Dalgetty, D, Thompson, AI, Newsome, P, Forbes, SJ, Ross, JA, Bradley, M & Iredale, JP 2011, 'Unbiased screening of polymer libraries to define novel substrates for functional hepatocytes with inducible drug metabolism', Stem Cell Research, vol. 6, no. 2, pp. 92-102. https://doi.org/10.1016/j.scr.2010.12.002

APA

Hay, DC., Pernagallo, S., Diaz-Mochon, JJ., Medine, CN., Greenhough, S., Hannoun, Z., Schrader, J., Black, JR., Fletcher, J., Dalgetty, D., Thompson, AI., Newsome, P., Forbes, SJ., Ross, JA., Bradley, M., & Iredale, JP. (2011). Unbiased screening of polymer libraries to define novel substrates for functional hepatocytes with inducible drug metabolism. Stem Cell Research, 6(2), 92-102. https://doi.org/10.1016/j.scr.2010.12.002

Vancouver

Author

Hay, DC ; Pernagallo, S ; Diaz-Mochon, JJ ; Medine, CN ; Greenhough, S ; Hannoun, Z ; Schrader, J ; Black, JR ; Fletcher, J ; Dalgetty, D ; Thompson, AI ; Newsome, Philip ; Forbes, SJ ; Ross, JA ; Bradley, M ; Iredale, JP. / Unbiased screening of polymer libraries to define novel substrates for functional hepatocytes with inducible drug metabolism. In: Stem Cell Research. 2011 ; Vol. 6, No. 2. pp. 92-102.

Bibtex

@article{e0a63ea96fd8407aada760d882b2bac4,
title = "Unbiased screening of polymer libraries to define novel substrates for functional hepatocytes with inducible drug metabolism",
abstract = "Maintaining stable differentiated somatic cell function in culture is essential to a range of biological endeavors. However, current technologies, employing, for example, primary hepatic cell culture (essential to the development of a bio-artificial liver and improved drug and toxicology testing), are limited by supply, expense, and functional instability even on biological cell culture substrata. As such, novel biologically active substrates manufacturable to GMP standards have the potential to improve cell culture-based assay applications. Currently hepatic endoderm (HE) generated from pluripotent stem cells is a genotypically diverse, cheap, and stable source of {"}hepatocytes{"}; however, HE routine applications are limited due to phenotypic instability in culture. Therefore a manufacturable subcellular matrix capable of supporting long-term differentiated cell function would represent a step forward in developing scalable and phenotypically stable hESC-derived hepatocytes. Adopting an unbiased approach we screened polymer microarrays and identified a polyurethane matrix which promoted HE viability, hepatocellular gene expression, drug-inducible metabolism, and function. Moreover, the polyurethane supported, when coated on a clinically approved bio-artificial liver matrix, long-term hepatocyte function and growth. In conclusion, our data suggest that an unbiased screening approach can identify cell culture substrate(s) that enhance the phenotypic stability of primary and stem cell-derived cell resources. (c) 2010 Elsevier B.V. All rights reserved.",
author = "DC Hay and S Pernagallo and JJ Diaz-Mochon and CN Medine and S Greenhough and Z Hannoun and J Schrader and JR Black and J Fletcher and D Dalgetty and AI Thompson and Philip Newsome and SJ Forbes and JA Ross and M Bradley and JP Iredale",
year = "2011",
month = mar,
day = "1",
doi = "10.1016/j.scr.2010.12.002",
language = "English",
volume = "6",
pages = "92--102",
journal = "Stem Cell Research",
issn = "1873-5061",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Unbiased screening of polymer libraries to define novel substrates for functional hepatocytes with inducible drug metabolism

AU - Hay, DC

AU - Pernagallo, S

AU - Diaz-Mochon, JJ

AU - Medine, CN

AU - Greenhough, S

AU - Hannoun, Z

AU - Schrader, J

AU - Black, JR

AU - Fletcher, J

AU - Dalgetty, D

AU - Thompson, AI

AU - Newsome, Philip

AU - Forbes, SJ

AU - Ross, JA

AU - Bradley, M

AU - Iredale, JP

PY - 2011/3/1

Y1 - 2011/3/1

N2 - Maintaining stable differentiated somatic cell function in culture is essential to a range of biological endeavors. However, current technologies, employing, for example, primary hepatic cell culture (essential to the development of a bio-artificial liver and improved drug and toxicology testing), are limited by supply, expense, and functional instability even on biological cell culture substrata. As such, novel biologically active substrates manufacturable to GMP standards have the potential to improve cell culture-based assay applications. Currently hepatic endoderm (HE) generated from pluripotent stem cells is a genotypically diverse, cheap, and stable source of "hepatocytes"; however, HE routine applications are limited due to phenotypic instability in culture. Therefore a manufacturable subcellular matrix capable of supporting long-term differentiated cell function would represent a step forward in developing scalable and phenotypically stable hESC-derived hepatocytes. Adopting an unbiased approach we screened polymer microarrays and identified a polyurethane matrix which promoted HE viability, hepatocellular gene expression, drug-inducible metabolism, and function. Moreover, the polyurethane supported, when coated on a clinically approved bio-artificial liver matrix, long-term hepatocyte function and growth. In conclusion, our data suggest that an unbiased screening approach can identify cell culture substrate(s) that enhance the phenotypic stability of primary and stem cell-derived cell resources. (c) 2010 Elsevier B.V. All rights reserved.

AB - Maintaining stable differentiated somatic cell function in culture is essential to a range of biological endeavors. However, current technologies, employing, for example, primary hepatic cell culture (essential to the development of a bio-artificial liver and improved drug and toxicology testing), are limited by supply, expense, and functional instability even on biological cell culture substrata. As such, novel biologically active substrates manufacturable to GMP standards have the potential to improve cell culture-based assay applications. Currently hepatic endoderm (HE) generated from pluripotent stem cells is a genotypically diverse, cheap, and stable source of "hepatocytes"; however, HE routine applications are limited due to phenotypic instability in culture. Therefore a manufacturable subcellular matrix capable of supporting long-term differentiated cell function would represent a step forward in developing scalable and phenotypically stable hESC-derived hepatocytes. Adopting an unbiased approach we screened polymer microarrays and identified a polyurethane matrix which promoted HE viability, hepatocellular gene expression, drug-inducible metabolism, and function. Moreover, the polyurethane supported, when coated on a clinically approved bio-artificial liver matrix, long-term hepatocyte function and growth. In conclusion, our data suggest that an unbiased screening approach can identify cell culture substrate(s) that enhance the phenotypic stability of primary and stem cell-derived cell resources. (c) 2010 Elsevier B.V. All rights reserved.

U2 - 10.1016/j.scr.2010.12.002

DO - 10.1016/j.scr.2010.12.002

M3 - Article

C2 - 21277274

VL - 6

SP - 92

EP - 102

JO - Stem Cell Research

JF - Stem Cell Research

SN - 1873-5061

IS - 2

ER -