Ultradian hormone stimulation induces glucocorticoid receptor-mediated pulses of gene transcription

Research output: Contribution to journalArticle

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Ultradian hormone stimulation induces glucocorticoid receptor-mediated pulses of gene transcription. / Stavreva, DA; Wiench, Malgorzata; John, Suganthi; Conway-Campbell, BL; McKenna, MA; Pooley, JR; Johnson, Thomas; Voss, TC; Lightman, SL; Hager, GL.

In: Nature Cell Biology, Vol. 11, No. 9, 01.09.2009, p. 1093-1102.

Research output: Contribution to journalArticle

Harvard

Stavreva, DA, Wiench, M, John, S, Conway-Campbell, BL, McKenna, MA, Pooley, JR, Johnson, T, Voss, TC, Lightman, SL & Hager, GL 2009, 'Ultradian hormone stimulation induces glucocorticoid receptor-mediated pulses of gene transcription', Nature Cell Biology, vol. 11, no. 9, pp. 1093-1102. https://doi.org/10.1038/ncb1922

APA

Stavreva, DA., Wiench, M., John, S., Conway-Campbell, BL., McKenna, MA., Pooley, JR., Johnson, T., Voss, TC., Lightman, SL., & Hager, GL. (2009). Ultradian hormone stimulation induces glucocorticoid receptor-mediated pulses of gene transcription. Nature Cell Biology, 11(9), 1093-1102. https://doi.org/10.1038/ncb1922

Vancouver

Author

Stavreva, DA ; Wiench, Malgorzata ; John, Suganthi ; Conway-Campbell, BL ; McKenna, MA ; Pooley, JR ; Johnson, Thomas ; Voss, TC ; Lightman, SL ; Hager, GL. / Ultradian hormone stimulation induces glucocorticoid receptor-mediated pulses of gene transcription. In: Nature Cell Biology. 2009 ; Vol. 11, No. 9. pp. 1093-1102.

Bibtex

@article{3ae361aab56040ab834ef3a50fa5fa6e,
title = "Ultradian hormone stimulation induces glucocorticoid receptor-mediated pulses of gene transcription",
abstract = "Studies on glucocorticoid receptor (GR) action typically assess gene responses by long-term stimulation with synthetic hormones. As corticosteroids are released from adrenal glands in a circadian and high-frequency (ultradian) mode, such treatments may not provide an accurate assessment of physiological hormone action. Here we demonstrate that ultradian hormone stimulation induces cyclic GR-mediated transcriptional regulation, or gene pulsing, both in cultured cells and in animal models. Equilibrium receptor-occupancy of regulatory elements precisely tracks the ligand pulses. Nascent RNA transcripts from GR-regulated genes are released in distinct quanta, demonstrating a profound difference between the transcriptional programs induced by ultradian and constant stimulation. Gene pulsing is driven by rapid GR exchange with response elements and by GR recycling through the chaperone machinery, which promotes GR activation and reactivation in response to the ultradian hormone release, thus coupling promoter activity to the naturally occurring fluctuations in hormone levels. The GR signalling pathway has been optimized for a prompt and timely response to fluctuations in hormone levels, indicating that biologically accurate regulation of gene targets by GR requires an ultradian mode of hormone stimulation.",
author = "DA Stavreva and Malgorzata Wiench and Suganthi John and BL Conway-Campbell and MA McKenna and JR Pooley and Thomas Johnson and TC Voss and SL Lightman and GL Hager",
year = "2009",
month = sep,
day = "1",
doi = "10.1038/ncb1922",
language = "English",
volume = "11",
pages = "1093--1102",
journal = "Nature Cell Biology",
issn = "1465-7392",
publisher = "Nature Publishing Group",
number = "9",

}

RIS

TY - JOUR

T1 - Ultradian hormone stimulation induces glucocorticoid receptor-mediated pulses of gene transcription

AU - Stavreva, DA

AU - Wiench, Malgorzata

AU - John, Suganthi

AU - Conway-Campbell, BL

AU - McKenna, MA

AU - Pooley, JR

AU - Johnson, Thomas

AU - Voss, TC

AU - Lightman, SL

AU - Hager, GL

PY - 2009/9/1

Y1 - 2009/9/1

N2 - Studies on glucocorticoid receptor (GR) action typically assess gene responses by long-term stimulation with synthetic hormones. As corticosteroids are released from adrenal glands in a circadian and high-frequency (ultradian) mode, such treatments may not provide an accurate assessment of physiological hormone action. Here we demonstrate that ultradian hormone stimulation induces cyclic GR-mediated transcriptional regulation, or gene pulsing, both in cultured cells and in animal models. Equilibrium receptor-occupancy of regulatory elements precisely tracks the ligand pulses. Nascent RNA transcripts from GR-regulated genes are released in distinct quanta, demonstrating a profound difference between the transcriptional programs induced by ultradian and constant stimulation. Gene pulsing is driven by rapid GR exchange with response elements and by GR recycling through the chaperone machinery, which promotes GR activation and reactivation in response to the ultradian hormone release, thus coupling promoter activity to the naturally occurring fluctuations in hormone levels. The GR signalling pathway has been optimized for a prompt and timely response to fluctuations in hormone levels, indicating that biologically accurate regulation of gene targets by GR requires an ultradian mode of hormone stimulation.

AB - Studies on glucocorticoid receptor (GR) action typically assess gene responses by long-term stimulation with synthetic hormones. As corticosteroids are released from adrenal glands in a circadian and high-frequency (ultradian) mode, such treatments may not provide an accurate assessment of physiological hormone action. Here we demonstrate that ultradian hormone stimulation induces cyclic GR-mediated transcriptional regulation, or gene pulsing, both in cultured cells and in animal models. Equilibrium receptor-occupancy of regulatory elements precisely tracks the ligand pulses. Nascent RNA transcripts from GR-regulated genes are released in distinct quanta, demonstrating a profound difference between the transcriptional programs induced by ultradian and constant stimulation. Gene pulsing is driven by rapid GR exchange with response elements and by GR recycling through the chaperone machinery, which promotes GR activation and reactivation in response to the ultradian hormone release, thus coupling promoter activity to the naturally occurring fluctuations in hormone levels. The GR signalling pathway has been optimized for a prompt and timely response to fluctuations in hormone levels, indicating that biologically accurate regulation of gene targets by GR requires an ultradian mode of hormone stimulation.

U2 - 10.1038/ncb1922

DO - 10.1038/ncb1922

M3 - Article

C2 - 19684579

VL - 11

SP - 1093

EP - 1102

JO - Nature Cell Biology

JF - Nature Cell Biology

SN - 1465-7392

IS - 9

ER -