TTF1 is membrane associated in breast carcinoma cell line MCF-7

L-S Siu; PD Abel; Hanna Romanska; T Kayademir; N Blin; GWH Stamp; El-Nasir Lalani

doi:10.1016/j.peptides.2004.01.024

TTF1 is membrane associated in breast carcinoma cell line MCF-7

L-S Siu, PD Abel, Hanna Romanska, T Kayademir, N Blin, GWH Stamp, El-Nasir Lalani

Cancer and Genomic Sciences

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2 Citations (Scopus)

Abstract

Trefoil factor family (TFF) domain peptides, products of mucin-secreting epithelial cells, are thought to influence mucosal integrity. Molecular studies revealed that mammalian TFFs lack transmembrane domains. Using immunocytochemistry and FACS analysis we demonstrated the association of TFF1 with the cell membrane in MCF-7 (a breast adenocarcinoma cell line), and tested the hypothesis that glycosylphosphatidylinositol (GPI) linkage is the mechanism for this association. Cleavage of GPI anchorage using phospholipase C did not affect TFF1 binding to the cell membrane. Our results demonstrate for the first time that TFF1 is associated with the cell membrane of MCF-7 cells and is not linked via a GPI anchor.

Original language	English
Pages (from-to)	745-753
Number of pages	9
Journal	Peptides
Volume	25
DOIs	https://doi.org/10.1016/j.peptides.2004.01.024
Publication status	Published - 1 May 2004

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10.1016/j.peptides.2004.01.024

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title = "TTF1 is membrane associated in breast carcinoma cell line MCF-7",

abstract = "Trefoil factor family (TFF) domain peptides, products of mucin-secreting epithelial cells, are thought to influence mucosal integrity. Molecular studies revealed that mammalian TFFs lack transmembrane domains. Using immunocytochemistry and FACS analysis we demonstrated the association of TFF1 with the cell membrane in MCF-7 (a breast adenocarcinoma cell line), and tested the hypothesis that glycosylphosphatidylinositol (GPI) linkage is the mechanism for this association. Cleavage of GPI anchorage using phospholipase C did not affect TFF1 binding to the cell membrane. Our results demonstrate for the first time that TFF1 is associated with the cell membrane of MCF-7 cells and is not linked via a GPI anchor.",

author = "L-S Siu and PD Abel and Hanna Romanska and T Kayademir and N Blin and GWH Stamp and El-Nasir Lalani",

year = "2004",

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doi = "10.1016/j.peptides.2004.01.024",

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TY - JOUR

T1 - TTF1 is membrane associated in breast carcinoma cell line MCF-7

AU - Siu, L-S

AU - Abel, PD

AU - Romanska, Hanna

AU - Kayademir, T

AU - Blin, N

AU - Stamp, GWH

AU - Lalani, El-Nasir

PY - 2004/5/1

Y1 - 2004/5/1

N2 - Trefoil factor family (TFF) domain peptides, products of mucin-secreting epithelial cells, are thought to influence mucosal integrity. Molecular studies revealed that mammalian TFFs lack transmembrane domains. Using immunocytochemistry and FACS analysis we demonstrated the association of TFF1 with the cell membrane in MCF-7 (a breast adenocarcinoma cell line), and tested the hypothesis that glycosylphosphatidylinositol (GPI) linkage is the mechanism for this association. Cleavage of GPI anchorage using phospholipase C did not affect TFF1 binding to the cell membrane. Our results demonstrate for the first time that TFF1 is associated with the cell membrane of MCF-7 cells and is not linked via a GPI anchor.

AB - Trefoil factor family (TFF) domain peptides, products of mucin-secreting epithelial cells, are thought to influence mucosal integrity. Molecular studies revealed that mammalian TFFs lack transmembrane domains. Using immunocytochemistry and FACS analysis we demonstrated the association of TFF1 with the cell membrane in MCF-7 (a breast adenocarcinoma cell line), and tested the hypothesis that glycosylphosphatidylinositol (GPI) linkage is the mechanism for this association. Cleavage of GPI anchorage using phospholipase C did not affect TFF1 binding to the cell membrane. Our results demonstrate for the first time that TFF1 is associated with the cell membrane of MCF-7 cells and is not linked via a GPI anchor.

UR - http://www.scopus.com/inward/record.url?scp=2642557775&partnerID=8YFLogxK

U2 - 10.1016/j.peptides.2004.01.024

DO - 10.1016/j.peptides.2004.01.024

M3 - Article

C2 - 15177868

VL - 25

SP - 745

EP - 753

JO - Peptides

JF - Peptides

ER -

TTF1 is membrane associated in breast carcinoma cell line MCF-7

Abstract

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