tRNA 3' processing in yeast involves tRNase Z, Rex1, and Rrp6

Research output: Contribution to journalArticlepeer-review

Authors

Colleges, School and Institutes

Abstract

Mature tRNA 3' ends in the yeast Saccharomyces cerevisiae are generated by two pathways: endonucleolytic and exonucleolytic. Although two exonucleases, Rex1 and Rrp6, have been shown to be responsible for the exonucleolytic trimming, the identity of the endonuclease has been inferred from other systems but not confirmed in vivo. Here, we show that the yeast tRNA 3' endonuclease tRNase Z, Trz1, is catalyzing endonucleolytic tRNA 3' processing. The majority of analyzed tRNAs utilize both pathways, with a preference for the endonucleolytic one. However, 3'-end processing of precursors with long 3' trailers depends to a greater extent on Trz1. In addition to its function in the nucleus, Trz1 processes the 3' ends of mitochondrial tRNAs, contributing to the general RNA metabolism in this organelle.

Details

Original languageEnglish
Pages (from-to)115-30
Number of pages16
JournalRNA
Volume20
Issue number1
Early online date18 Nov 2013
Publication statusPublished - Jan 2014

Keywords

  • Cell Nucleus, Endoribonucleases, Exoribonucleases, Exosome Multienzyme Ribonuclease Complex, Gene Silencing, Metabolic Networks and Pathways, Mitochondria, Organisms, Genetically Modified, RNA, RNA 3' End Processing, RNA, Transfer, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Journal Article, tRNA, tRNase Z, 3′ -end processing, exonucleases