Transposition and targeting of the prokaryotic mobile element IS30 in zebrafish

Research output: Contribution to journalArticlepeer-review

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Transposition and targeting of the prokaryotic mobile element IS30 in zebrafish. / Szabó, Mónika; Müller, Ferenc; Kiss, János; Balduf, Carolin; Strähle, Uwe; Olasz, Ferenc.

In: FEBS Letters, Vol. 550, No. 1-3, 28.08.2003, p. 46-50.

Research output: Contribution to journalArticlepeer-review

Harvard

Szabó, M, Müller, F, Kiss, J, Balduf, C, Strähle, U & Olasz, F 2003, 'Transposition and targeting of the prokaryotic mobile element IS30 in zebrafish', FEBS Letters, vol. 550, no. 1-3, pp. 46-50.

APA

Szabó, M., Müller, F., Kiss, J., Balduf, C., Strähle, U., & Olasz, F. (2003). Transposition and targeting of the prokaryotic mobile element IS30 in zebrafish. FEBS Letters, 550(1-3), 46-50.

Vancouver

Szabó M, Müller F, Kiss J, Balduf C, Strähle U, Olasz F. Transposition and targeting of the prokaryotic mobile element IS30 in zebrafish. FEBS Letters. 2003 Aug 28;550(1-3):46-50.

Author

Szabó, Mónika ; Müller, Ferenc ; Kiss, János ; Balduf, Carolin ; Strähle, Uwe ; Olasz, Ferenc. / Transposition and targeting of the prokaryotic mobile element IS30 in zebrafish. In: FEBS Letters. 2003 ; Vol. 550, No. 1-3. pp. 46-50.

Bibtex

@article{0224e3b7122c4e82b1b0455ec4836b76,
title = "Transposition and targeting of the prokaryotic mobile element IS30 in zebrafish",
abstract = "We provide evidence that a prokaryotic insertion sequence (IS) element is active in a vertebrate system. The transposase of Escherichia coli element IS30 catalyzes both excision and integration in extrachromosomal DNA in zebrafish embryos. The transposase has a pronounced target preference, which is shown to be modified by fusing the enzyme to unrelated DNA binding proteins. Joining the transposase to the cI repressor of phage lambda causes transposition primarily into the vicinity of the lambda operator in E. coli, and linking to the DNA binding domain of Gli1 also directs the recombination activity of transposase near to the Gli1 binding site in zebrafish. Our results demonstrate the possibility of fusion transposases to acquire novel target specificity in both prokaryotes and eukaryotes.",
keywords = "Animals, Binding Sites, DNA, DNA Transposable Elements, DNA-Binding Proteins, Embryo, Nonmammalian, Escherichia coli, Genetic Techniques, HeLa Cells, Humans, Oncogene Proteins, Prokaryotic Cells, Recombination, Genetic, Repressor Proteins, Substrate Specificity, Trans-Activators, Transcription Factors, Transposases, Viral Proteins, Viral Regulatory and Accessory Proteins, Zebrafish",
author = "M{\'o}nika Szab{\'o} and Ferenc M{\"u}ller and J{\'a}nos Kiss and Carolin Balduf and Uwe Str{\"a}hle and Ferenc Olasz",
year = "2003",
month = aug,
day = "28",
language = "English",
volume = "550",
pages = "46--50",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1-3",

}

RIS

TY - JOUR

T1 - Transposition and targeting of the prokaryotic mobile element IS30 in zebrafish

AU - Szabó, Mónika

AU - Müller, Ferenc

AU - Kiss, János

AU - Balduf, Carolin

AU - Strähle, Uwe

AU - Olasz, Ferenc

PY - 2003/8/28

Y1 - 2003/8/28

N2 - We provide evidence that a prokaryotic insertion sequence (IS) element is active in a vertebrate system. The transposase of Escherichia coli element IS30 catalyzes both excision and integration in extrachromosomal DNA in zebrafish embryos. The transposase has a pronounced target preference, which is shown to be modified by fusing the enzyme to unrelated DNA binding proteins. Joining the transposase to the cI repressor of phage lambda causes transposition primarily into the vicinity of the lambda operator in E. coli, and linking to the DNA binding domain of Gli1 also directs the recombination activity of transposase near to the Gli1 binding site in zebrafish. Our results demonstrate the possibility of fusion transposases to acquire novel target specificity in both prokaryotes and eukaryotes.

AB - We provide evidence that a prokaryotic insertion sequence (IS) element is active in a vertebrate system. The transposase of Escherichia coli element IS30 catalyzes both excision and integration in extrachromosomal DNA in zebrafish embryos. The transposase has a pronounced target preference, which is shown to be modified by fusing the enzyme to unrelated DNA binding proteins. Joining the transposase to the cI repressor of phage lambda causes transposition primarily into the vicinity of the lambda operator in E. coli, and linking to the DNA binding domain of Gli1 also directs the recombination activity of transposase near to the Gli1 binding site in zebrafish. Our results demonstrate the possibility of fusion transposases to acquire novel target specificity in both prokaryotes and eukaryotes.

KW - Animals

KW - Binding Sites

KW - DNA

KW - DNA Transposable Elements

KW - DNA-Binding Proteins

KW - Embryo, Nonmammalian

KW - Escherichia coli

KW - Genetic Techniques

KW - HeLa Cells

KW - Humans

KW - Oncogene Proteins

KW - Prokaryotic Cells

KW - Recombination, Genetic

KW - Repressor Proteins

KW - Substrate Specificity

KW - Trans-Activators

KW - Transcription Factors

KW - Transposases

KW - Viral Proteins

KW - Viral Regulatory and Accessory Proteins

KW - Zebrafish

M3 - Article

C2 - 12935884

VL - 550

SP - 46

EP - 50

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1-3

ER -