Transient reduction in IgA+ and IgG+ memory B cell numbers in young EBV-seropositive children: the Generation R Study

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Transient reduction in IgA+ and IgG+ memory B cell numbers in young EBV-seropositive children : the Generation R Study. / van den Heuvel, Diana; Jansen, Michelle A E; Bell, Andrew I; Rickinson, Alan B; Jaddoe, Vincent W V; van Dongen, Jacques J M; Moll, Henriette A; van Zelm, Menno C.

In: Journal of Leukocyte Biology, Vol. 101, No. 4, 04.2017, p. 949-956.

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van den Heuvel, Diana ; Jansen, Michelle A E ; Bell, Andrew I ; Rickinson, Alan B ; Jaddoe, Vincent W V ; van Dongen, Jacques J M ; Moll, Henriette A ; van Zelm, Menno C. / Transient reduction in IgA+ and IgG+ memory B cell numbers in young EBV-seropositive children : the Generation R Study. In: Journal of Leukocyte Biology. 2017 ; Vol. 101, No. 4. pp. 949-956.

Bibtex

@article{fd8cf35693d941e5b1f52ad8823d3c51,
title = "Transient reduction in IgA+ and IgG+ memory B cell numbers in young EBV-seropositive children: the Generation R Study",
abstract = "The EBV is known to persist in memory B cells, but it remains unclear how this affects cell numbers and humoral immunity. We here studied EBV persistence in memory B cell subsets and consequences on B cell memory in young children. EBV genome loads were quantified in 6 memory B cell subsets in EBV(+) adults. The effects of EBV infection on memory B cell numbers and vaccination responses were studied longitudinally in children within the Generation R population cohort between 14 mo and 6 yr of age. EBV genomes were more numerous in CD27(+)IgG(+), CD27(+)IgA(+), and CD27(-)IgA(+) memory B cells than in IgM-only, natural effector, and CD27(-)IgG(+) B cells. The blood counts of IgM-only, CD27(+)IgA(+), CD27(-)IgG(+), and CD27(+)IgG(+) memory B cells were significantly lower in EBV(+) children than in uninfected controls at 14 mo of age-the age when these cells peak in numbers. At 6 yr, all of these memory B cell counts had normalized, as had plasma IgG levels to previous primary measles and booster tetanus vaccinations. In conclusion, EBV persists predominantly in Ig class-switched memory B cells, even when derived from T cell-independent responses (CD27(-)IgA(+)), and EBV infection results in a transient depletion of these cells in young children.",
keywords = "pediatric infection, herpes virus, humoral immunity, Epstein-Barr virus",
author = "{van den Heuvel}, Diana and Jansen, {Michelle A E} and Bell, {Andrew I} and Rickinson, {Alan B} and Jaddoe, {Vincent W V} and {van Dongen}, {Jacques J M} and Moll, {Henriette A} and {van Zelm}, {Menno C}",
note = "{\textcopyright} Society for Leukocyte Biology.",
year = "2017",
month = apr
doi = "10.1189/jlb.5VMAB0616-283R",
language = "English",
volume = "101",
pages = "949--956",
journal = "Journal of Leukocyte Biology",
issn = "0741-5400",
publisher = "Society for Leukocyte Biology",
number = "4",

}

RIS

TY - JOUR

T1 - Transient reduction in IgA+ and IgG+ memory B cell numbers in young EBV-seropositive children

T2 - the Generation R Study

AU - van den Heuvel, Diana

AU - Jansen, Michelle A E

AU - Bell, Andrew I

AU - Rickinson, Alan B

AU - Jaddoe, Vincent W V

AU - van Dongen, Jacques J M

AU - Moll, Henriette A

AU - van Zelm, Menno C

N1 - © Society for Leukocyte Biology.

PY - 2017/4

Y1 - 2017/4

N2 - The EBV is known to persist in memory B cells, but it remains unclear how this affects cell numbers and humoral immunity. We here studied EBV persistence in memory B cell subsets and consequences on B cell memory in young children. EBV genome loads were quantified in 6 memory B cell subsets in EBV(+) adults. The effects of EBV infection on memory B cell numbers and vaccination responses were studied longitudinally in children within the Generation R population cohort between 14 mo and 6 yr of age. EBV genomes were more numerous in CD27(+)IgG(+), CD27(+)IgA(+), and CD27(-)IgA(+) memory B cells than in IgM-only, natural effector, and CD27(-)IgG(+) B cells. The blood counts of IgM-only, CD27(+)IgA(+), CD27(-)IgG(+), and CD27(+)IgG(+) memory B cells were significantly lower in EBV(+) children than in uninfected controls at 14 mo of age-the age when these cells peak in numbers. At 6 yr, all of these memory B cell counts had normalized, as had plasma IgG levels to previous primary measles and booster tetanus vaccinations. In conclusion, EBV persists predominantly in Ig class-switched memory B cells, even when derived from T cell-independent responses (CD27(-)IgA(+)), and EBV infection results in a transient depletion of these cells in young children.

AB - The EBV is known to persist in memory B cells, but it remains unclear how this affects cell numbers and humoral immunity. We here studied EBV persistence in memory B cell subsets and consequences on B cell memory in young children. EBV genome loads were quantified in 6 memory B cell subsets in EBV(+) adults. The effects of EBV infection on memory B cell numbers and vaccination responses were studied longitudinally in children within the Generation R population cohort between 14 mo and 6 yr of age. EBV genomes were more numerous in CD27(+)IgG(+), CD27(+)IgA(+), and CD27(-)IgA(+) memory B cells than in IgM-only, natural effector, and CD27(-)IgG(+) B cells. The blood counts of IgM-only, CD27(+)IgA(+), CD27(-)IgG(+), and CD27(+)IgG(+) memory B cells were significantly lower in EBV(+) children than in uninfected controls at 14 mo of age-the age when these cells peak in numbers. At 6 yr, all of these memory B cell counts had normalized, as had plasma IgG levels to previous primary measles and booster tetanus vaccinations. In conclusion, EBV persists predominantly in Ig class-switched memory B cells, even when derived from T cell-independent responses (CD27(-)IgA(+)), and EBV infection results in a transient depletion of these cells in young children.

KW - pediatric infection

KW - herpes virus

KW - humoral immunity

KW - Epstein-Barr virus

U2 - 10.1189/jlb.5VMAB0616-283R

DO - 10.1189/jlb.5VMAB0616-283R

M3 - Article

C2 - 27821468

VL - 101

SP - 949

EP - 956

JO - Journal of Leukocyte Biology

JF - Journal of Leukocyte Biology

SN - 0741-5400

IS - 4

ER -