Transcriptome characterisation and SSR markers development in the seagrass Posidonia oceanica

Research output: Contribution to journalArticle

Authors

  • Daniela D'Esposito
  • Luigi Orru
  • Emanuela Dattolo
  • Letizia Bernardo
  • Antonella Lamontanara
  • Ilia Serra
  • Silvia Mazzucca
  • Gabriele Procaccini

Colleges, School and Institutes

External organisations

  • Stazione Zoologica Anton Dohrn, Villa Comunale, 80121, Napoli, Italy
  • Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Centro di ricerca per la genomica vegetale, 29017, Fiorenzuola d’Adda, Italy
  • Stazione Zoologica Anton Dohrn, Villa Comunale, 80121, Napoli, Italy
  • Laboratorio di Biologia e Proteomica Vegetale (Lab. Bio. Pro. Ve.), Dipartimento di Chimica e Tecnologie Chimiche, Università della Calabria, 87036 Rende (CS), Italy

Abstract

Posidonia oceanica is an endemic seagrass in the Mediterranean Sea, where it provides important ecosystem services, as absorbing and storing CO2 and protecting the coastline from erosion, and sustains a rich and diverse ecosystem. P. oceanica meadows extend from the surface to 40 meters depth. With the aim of boosting research in this iconic species, we generated a
comprehensive RNA-Seq data set for P. oceanica by sequencing specimens collected at different depths and at different times during the day. With this approach we attempted to capture the transcriptional diversity associated with change in light and other depth-related environmental factors. Using this extensive data set we generated gene predictions and identified an extensive
catalogue of SSR markers. The data generated here will open new avenues for the analysis of population genetic features and functional variation in P. oceanica. In total, 79,235 contigs were obtained by the assembly of 70,453120 paired end reads. 43,711contigs were successfully annotated. A total of 17,436 SSR were identified within 13,912 contigs

Details

Original languageEnglish
Article number160115
JournalScientific Data
Volume3
Publication statusPublished - 20 Dec 2016