Transcription of the plasmid-encoded toxin gene from Enteroaggregative Escherichia coli is regulated by a novel co-activation mechanism involving CRP and Fis.

Research output: Contribution to journalArticle

Standard

Harvard

APA

Vancouver

Author

Bibtex

@article{6f5d976c7fa242fb9eb70a944a48bb6c,
title = "Transcription of the plasmid-encoded toxin gene from Enteroaggregative Escherichia coli is regulated by a novel co-activation mechanism involving CRP and Fis.",
abstract = "Enteroaggregative Escherichia coli (EAEC) is a major cause of diarrhoea in developing countries. EAEC 042 is the prototypical strain. EAEC 042 secretes the functionally well-characterised Pet autotransporter toxin that contributes to virulence through its cytotoxic effects on intestinal epithelial cells. Following a global transposon mutagenesis screen of EAEC 042, the transcription factors, CRP and Fis, were identified as essential for transcription of the pet gene. Using both in vivo and in vitro techniques, we show that the pet promoter is co-dependent on CRP and Fis. We present a novel co-activation mechanism whereby CRP is placed at a non-optimal position for transcription initiation, creating dependence on Fis for full activation of pet. This study complements previous findings that establish Fis as a key virulence regulator in EAEC 042.",
author = "Amanda Rossiter and Douglas Browning and Denisse Leyton and Matthew Johnson and Rita Godfrey and Catherine Wardius and M Desvaux and Adam Cunningham and F Ruiz-Perez and JP Nataro and Stephen Busby and Ian Henderson",
year = "2011",
month = may
day = "4",
doi = "10.1111/j.1365-2958.2011.07685.x",
language = "English",
volume = "81",
pages = "179--191",
journal = "Molecular Microbiology",
issn = "0950-382X",
publisher = "Wiley",
number = "1",

}

RIS

TY - JOUR

T1 - Transcription of the plasmid-encoded toxin gene from Enteroaggregative Escherichia coli is regulated by a novel co-activation mechanism involving CRP and Fis.

AU - Rossiter, Amanda

AU - Browning, Douglas

AU - Leyton, Denisse

AU - Johnson, Matthew

AU - Godfrey, Rita

AU - Wardius, Catherine

AU - Desvaux, M

AU - Cunningham, Adam

AU - Ruiz-Perez, F

AU - Nataro, JP

AU - Busby, Stephen

AU - Henderson, Ian

PY - 2011/5/4

Y1 - 2011/5/4

N2 - Enteroaggregative Escherichia coli (EAEC) is a major cause of diarrhoea in developing countries. EAEC 042 is the prototypical strain. EAEC 042 secretes the functionally well-characterised Pet autotransporter toxin that contributes to virulence through its cytotoxic effects on intestinal epithelial cells. Following a global transposon mutagenesis screen of EAEC 042, the transcription factors, CRP and Fis, were identified as essential for transcription of the pet gene. Using both in vivo and in vitro techniques, we show that the pet promoter is co-dependent on CRP and Fis. We present a novel co-activation mechanism whereby CRP is placed at a non-optimal position for transcription initiation, creating dependence on Fis for full activation of pet. This study complements previous findings that establish Fis as a key virulence regulator in EAEC 042.

AB - Enteroaggregative Escherichia coli (EAEC) is a major cause of diarrhoea in developing countries. EAEC 042 is the prototypical strain. EAEC 042 secretes the functionally well-characterised Pet autotransporter toxin that contributes to virulence through its cytotoxic effects on intestinal epithelial cells. Following a global transposon mutagenesis screen of EAEC 042, the transcription factors, CRP and Fis, were identified as essential for transcription of the pet gene. Using both in vivo and in vitro techniques, we show that the pet promoter is co-dependent on CRP and Fis. We present a novel co-activation mechanism whereby CRP is placed at a non-optimal position for transcription initiation, creating dependence on Fis for full activation of pet. This study complements previous findings that establish Fis as a key virulence regulator in EAEC 042.

U2 - 10.1111/j.1365-2958.2011.07685.x

DO - 10.1111/j.1365-2958.2011.07685.x

M3 - Article

C2 - 21542864

VL - 81

SP - 179

EP - 191

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

IS - 1

ER -