Transcription factor distribution in Escherichia coli: studies with FNR protein

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Colleges, School and Institutes

Abstract

Using chromatin immunoprecipitation (ChIP) and high-density microarrays, we have measured the distribution of the global transcription regulator protein, FNR, across the entire Escherichia coli chromosome in exponentially growing cells. Sixty-three binding targets, each located at the 5' end of a gene, were identified. Some targets are adjacent to poorly transcribed genes where FNR has little impact on transcription. In stationary phase, the distribution of FNR was largely unchanged. Control experiments showed that, like FNR, the distribution of the nucleoid-associated protein, IHF, is little altered when cells enter stationary phase, whilst RNA polymerase undergoes a complete redistribution.

Details

Original languageEnglish
Pages (from-to)269-278
Number of pages10
JournalNucleic Acids Research
Volume35
Issue number1
Early online date12 Dec 2006
Publication statusPublished - 1 Jan 2007