Trafficking and Function of GPCRs in the Endosomal Compartment

Davide Calebiro; Amod Godbole; Sandra Lyga; Martin J. Lohse

doi:10.1007/978-1-4939-1755-6_16

Trafficking and Function of GPCRs in the Endosomal Compartment

Davide Calebiro, Amod Godbole, Sandra Lyga, Martin J. Lohse

Metabolism and Systems Research

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

New methods based on fluorescently labeled agonists, genetically encoded fluorescent sensors, and advanced microscopy techniques, such as fluorescence resonance energy transfer (FRET) and highly inclined thin illumination (HILO), allow direct monitoring of signaling, internalization, and intracellular trafficking of G protein-coupled receptors (GPCRs) and their ligands in living cells with high temporal and spatial resolution. These methods have been essential in revealing that GPCRs can continue signaling via production of the soluble second messenger cyclic AMP after internalization into the endosomal compartment.

Original language	English
Pages (from-to)	197-211
Number of pages	15
Journal	Methods in molecular biology
Volume	1234
Early online date	20 Sept 2014
DOIs	https://doi.org/10.1007/978-1-4939-1755-6_16
Publication status	Published - 2015

Keywords

Endocytosis inhibitor
Fluorescence resonance energy transfer
G protein-coupled receptor
Highly inclined thin illumination
Thyroid follicle
Thyroid stimulating hormone
Thyroid stimulating hormone receptor
Total internal reflection fluorescence microscopy

ASJC Scopus subject areas

Molecular Biology
Genetics

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https://doi.org/10.1007/978-1-4939-1755-6_16Licence: None: All rights reserved

Cite this

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title = "Trafficking and Function of GPCRs in the Endosomal Compartment",

abstract = "New methods based on fluorescently labeled agonists, genetically encoded fluorescent sensors, and advanced microscopy techniques, such as fluorescence resonance energy transfer (FRET) and highly inclined thin illumination (HILO), allow direct monitoring of signaling, internalization, and intracellular trafficking of G protein-coupled receptors (GPCRs) and their ligands in living cells with high temporal and spatial resolution. These methods have been essential in revealing that GPCRs can continue signaling via production of the soluble second messenger cyclic AMP after internalization into the endosomal compartment.",

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AU - Godbole, Amod

AU - Lyga, Sandra

AU - Lohse, Martin J.

PY - 2015

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AB - New methods based on fluorescently labeled agonists, genetically encoded fluorescent sensors, and advanced microscopy techniques, such as fluorescence resonance energy transfer (FRET) and highly inclined thin illumination (HILO), allow direct monitoring of signaling, internalization, and intracellular trafficking of G protein-coupled receptors (GPCRs) and their ligands in living cells with high temporal and spatial resolution. These methods have been essential in revealing that GPCRs can continue signaling via production of the soluble second messenger cyclic AMP after internalization into the endosomal compartment.

KW - Endocytosis inhibitor

KW - Fluorescence resonance energy transfer

KW - G protein-coupled receptor

KW - Highly inclined thin illumination

KW - Thyroid follicle

KW - Thyroid stimulating hormone

KW - Thyroid stimulating hormone receptor

KW - Total internal reflection fluorescence microscopy

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AN - SCOPUS:84921735230

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EP - 211

JO - Methods in molecular biology

JF - Methods in molecular biology

ER -

Trafficking and Function of GPCRs in the Endosomal Compartment

Abstract

Keywords

ASJC Scopus subject areas

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