Trafficking and Function of GPCRs in the Endosomal Compartment

Research output: Contribution to journalArticle

Authors

Colleges, School and Institutes

Abstract

New methods based on fl uorescently labeled agonists, genetically encoded fl uorescent sensors, and advanced microscopy techniques, such as fl uorescence resonance energy transfer (FRET) and highly inclined thin illumination (HILO), allow direct monitoring of signaling, internalization, and intracellular traffi cking of G protein-coupled receptors (GPCRs) and their ligands in living cells with high temporal and spatial resolution. These methods have been essential in revealing that GPCRs can continue signaling via production of the soluble second messenger cyclic AMP after internalization into the endosomal compartment.

Details

Original languageEnglish
Pages (from-to)197-211
Number of pages15
JournalMethods in molecular biology
Volume1234
Publication statusPublished - 2015

Keywords

  • Endocytosis inhibitor, Fluorescence resonance energy transfer, G protein-coupled receptor, Highly inclined thin illumination, Thyroid follicle, Thyroid stimulating hormone, Thyroid stimulating hormone receptor, Total internal refl ection fl uorescence microscopy

ASJC Scopus subject areas