Trafficking and Function of GPCRs in the Endosomal Compartment
Research output: Contribution to journal › Article
Colleges, School and Institutes
New methods based on fl uorescently labeled agonists, genetically encoded fl uorescent sensors, and advanced microscopy techniques, such as fl uorescence resonance energy transfer (FRET) and highly inclined thin illumination (HILO), allow direct monitoring of signaling, internalization, and intracellular traffi cking of G protein-coupled receptors (GPCRs) and their ligands in living cells with high temporal and spatial resolution. These methods have been essential in revealing that GPCRs can continue signaling via production of the soluble second messenger cyclic AMP after internalization into the endosomal compartment.
|Number of pages||15|
|Journal||Methods in molecular biology|
|Publication status||Published - 2015|
- Endocytosis inhibitor, Fluorescence resonance energy transfer, G protein-coupled receptor, Highly inclined thin illumination, Thyroid follicle, Thyroid stimulating hormone, Thyroid stimulating hormone receptor, Total internal refl ection fl uorescence microscopy