TY - JOUR
T1 - Tracing functionally identified neurones in multisynaptic pathway in the hamster and rat using herpes simplex virus expressing geen fluorescent protein
AU - Pyner, Susan
AU - Cleary, Jennifer
AU - Mcleish, Philippa
AU - Buchan, Alexander
AU - Coote, John
PY - 2001/11/1
Y1 - 2001/11/1
N2 - Using a genetically modified herpes simplex virus encoding green fluorescent protein we sought to establish if this viral modification could be used in transneuronal tracing studies of the sympathetic nervous system. The herpes simplex virus encoding green fluorescent protein was injected into the adrenal medulla of three hamsters and six rats. After a suitable survival period, neurones in the sympathetic intermediolateral cell column of the thoracolumbar spinal cord, rostral ventral medulla and paraventricular nucleus of the hypothalamus were clearly identified by the presence of a green fluorescence in the cytoplasm of the neurones of both species. Thus, herpes simplex virus encoding green fluorescent protein labelled chains of sympathetic neurones in the hamster and rat and therefore has the potential to be used in transneuronal tracing studies of autonomic pathways in these species.
AB - Using a genetically modified herpes simplex virus encoding green fluorescent protein we sought to establish if this viral modification could be used in transneuronal tracing studies of the sympathetic nervous system. The herpes simplex virus encoding green fluorescent protein was injected into the adrenal medulla of three hamsters and six rats. After a suitable survival period, neurones in the sympathetic intermediolateral cell column of the thoracolumbar spinal cord, rostral ventral medulla and paraventricular nucleus of the hypothalamus were clearly identified by the presence of a green fluorescence in the cytoplasm of the neurones of both species. Thus, herpes simplex virus encoding green fluorescent protein labelled chains of sympathetic neurones in the hamster and rat and therefore has the potential to be used in transneuronal tracing studies of autonomic pathways in these species.
UR - http://www.scopus.com/inward/record.url?scp=0035198041&partnerID=8YFLogxK
U2 - 10.1111/j.1469-445X.2001.tb00034.x
DO - 10.1111/j.1469-445X.2001.tb00034.x
M3 - Article
C2 - 11698963
SN - 1469-445X
VL - 86
SP - 695
EP - 702
JO - Experimental Physiology
JF - Experimental Physiology
IS - 6
ER -