Toxicological effect of single contaminants and contaminant mixtures associated with plant ingredients in novel salmon feeds

Research output: Contribution to journalArticlepeer-review

Standard

Toxicological effect of single contaminants and contaminant mixtures associated with plant ingredients in novel salmon feeds. / Søfteland, Liv; Kirwan, Jennifer A.; Hori, Tiago S F; Størseth, Trond R.; Sommer, Ulf; Berntssen, Marc H G; Viant, Mark R.; Rise, Matthew L.; Waagbø, Rune; Torstensen, Bente E.; Booman, Marije; Olsvik, Pål A.

In: Food and Chemical Toxicology, Vol. 73, 01.11.2014, p. 157-174.

Research output: Contribution to journalArticlepeer-review

Harvard

Søfteland, L, Kirwan, JA, Hori, TSF, Størseth, TR, Sommer, U, Berntssen, MHG, Viant, MR, Rise, ML, Waagbø, R, Torstensen, BE, Booman, M & Olsvik, PA 2014, 'Toxicological effect of single contaminants and contaminant mixtures associated with plant ingredients in novel salmon feeds', Food and Chemical Toxicology, vol. 73, pp. 157-174. https://doi.org/10.1016/j.fct.2014.08.008

APA

Søfteland, L., Kirwan, J. A., Hori, T. S. F., Størseth, T. R., Sommer, U., Berntssen, M. H. G., Viant, M. R., Rise, M. L., Waagbø, R., Torstensen, B. E., Booman, M., & Olsvik, P. A. (2014). Toxicological effect of single contaminants and contaminant mixtures associated with plant ingredients in novel salmon feeds. Food and Chemical Toxicology, 73, 157-174. https://doi.org/10.1016/j.fct.2014.08.008

Vancouver

Author

Søfteland, Liv ; Kirwan, Jennifer A. ; Hori, Tiago S F ; Størseth, Trond R. ; Sommer, Ulf ; Berntssen, Marc H G ; Viant, Mark R. ; Rise, Matthew L. ; Waagbø, Rune ; Torstensen, Bente E. ; Booman, Marije ; Olsvik, Pål A. / Toxicological effect of single contaminants and contaminant mixtures associated with plant ingredients in novel salmon feeds. In: Food and Chemical Toxicology. 2014 ; Vol. 73. pp. 157-174.

Bibtex

@article{cb041c47435042e9b413045a3d3565db,
title = "Toxicological effect of single contaminants and contaminant mixtures associated with plant ingredients in novel salmon feeds",
abstract = "Increasing use of plant feed ingredients may introduce contaminants not previously associated with farming of salmonids, such as pesticides and PAHs from environmental sources or from thermal processing of oil seeds. To screen for interaction effects of contaminants newly introduced in salmon feeds, Atlantic salmon primary hepatocytes were used. The xCELLigence cytotoxicity system was used to select optimal dosages of the PAHs benzo(a)pyrene and phenanthrene, the pesticides chlorpyrifos and endosulfan, and combinations of these. NMR and MS metabolic profiling and microarray transcriptomic profiling was used to identify novel biomarkers. Lipidomic and transcriptomic profiling suggested perturbation of lipid metabolism, as well as endocrine disruption. The pesticides gave the strongest responses, despite having less effect on cell viability than the PAHs. Only weak molecular responses were detected in PAH-exposed hepatocytes. Chlorpyrifos suppressed the synthesis of unsaturated fatty acids. Endosulfan affected steroid hormone synthesis, while benzo(a)pyrene disturbed vitamin D3 metabolism. The primary mixture effect was additive, although at high concentrations the pesticides acted in a synergistic fashion to decrease cell viability and down-regulate CYP3A and FABP4 transcription. This work highlights the usefulness of 'omics techniques and multivariate data analysis to investigate interactions within mixtures of contaminants with different modes of action.",
keywords = "Atlantic salmon, Metabolomics, PAH, Pesticides, Synergy, Toxicogenomics",
author = "Liv S{\o}fteland and Kirwan, {Jennifer A.} and Hori, {Tiago S F} and St{\o}rseth, {Trond R.} and Ulf Sommer and Berntssen, {Marc H G} and Viant, {Mark R.} and Rise, {Matthew L.} and Rune Waagb{\o} and Torstensen, {Bente E.} and Marije Booman and Olsvik, {P{\aa}l A.}",
year = "2014",
month = nov,
day = "1",
doi = "10.1016/j.fct.2014.08.008",
language = "English",
volume = "73",
pages = "157--174",
journal = "Food and Chemical Toxicology",
issn = "0278-6915",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Toxicological effect of single contaminants and contaminant mixtures associated with plant ingredients in novel salmon feeds

AU - Søfteland, Liv

AU - Kirwan, Jennifer A.

AU - Hori, Tiago S F

AU - Størseth, Trond R.

AU - Sommer, Ulf

AU - Berntssen, Marc H G

AU - Viant, Mark R.

AU - Rise, Matthew L.

AU - Waagbø, Rune

AU - Torstensen, Bente E.

AU - Booman, Marije

AU - Olsvik, Pål A.

PY - 2014/11/1

Y1 - 2014/11/1

N2 - Increasing use of plant feed ingredients may introduce contaminants not previously associated with farming of salmonids, such as pesticides and PAHs from environmental sources or from thermal processing of oil seeds. To screen for interaction effects of contaminants newly introduced in salmon feeds, Atlantic salmon primary hepatocytes were used. The xCELLigence cytotoxicity system was used to select optimal dosages of the PAHs benzo(a)pyrene and phenanthrene, the pesticides chlorpyrifos and endosulfan, and combinations of these. NMR and MS metabolic profiling and microarray transcriptomic profiling was used to identify novel biomarkers. Lipidomic and transcriptomic profiling suggested perturbation of lipid metabolism, as well as endocrine disruption. The pesticides gave the strongest responses, despite having less effect on cell viability than the PAHs. Only weak molecular responses were detected in PAH-exposed hepatocytes. Chlorpyrifos suppressed the synthesis of unsaturated fatty acids. Endosulfan affected steroid hormone synthesis, while benzo(a)pyrene disturbed vitamin D3 metabolism. The primary mixture effect was additive, although at high concentrations the pesticides acted in a synergistic fashion to decrease cell viability and down-regulate CYP3A and FABP4 transcription. This work highlights the usefulness of 'omics techniques and multivariate data analysis to investigate interactions within mixtures of contaminants with different modes of action.

AB - Increasing use of plant feed ingredients may introduce contaminants not previously associated with farming of salmonids, such as pesticides and PAHs from environmental sources or from thermal processing of oil seeds. To screen for interaction effects of contaminants newly introduced in salmon feeds, Atlantic salmon primary hepatocytes were used. The xCELLigence cytotoxicity system was used to select optimal dosages of the PAHs benzo(a)pyrene and phenanthrene, the pesticides chlorpyrifos and endosulfan, and combinations of these. NMR and MS metabolic profiling and microarray transcriptomic profiling was used to identify novel biomarkers. Lipidomic and transcriptomic profiling suggested perturbation of lipid metabolism, as well as endocrine disruption. The pesticides gave the strongest responses, despite having less effect on cell viability than the PAHs. Only weak molecular responses were detected in PAH-exposed hepatocytes. Chlorpyrifos suppressed the synthesis of unsaturated fatty acids. Endosulfan affected steroid hormone synthesis, while benzo(a)pyrene disturbed vitamin D3 metabolism. The primary mixture effect was additive, although at high concentrations the pesticides acted in a synergistic fashion to decrease cell viability and down-regulate CYP3A and FABP4 transcription. This work highlights the usefulness of 'omics techniques and multivariate data analysis to investigate interactions within mixtures of contaminants with different modes of action.

KW - Atlantic salmon

KW - Metabolomics

KW - PAH

KW - Pesticides

KW - Synergy

KW - Toxicogenomics

UR - http://www.scopus.com/inward/record.url?scp=84908153645&partnerID=8YFLogxK

U2 - 10.1016/j.fct.2014.08.008

DO - 10.1016/j.fct.2014.08.008

M3 - Article

AN - SCOPUS:84908153645

VL - 73

SP - 157

EP - 174

JO - Food and Chemical Toxicology

JF - Food and Chemical Toxicology

SN - 0278-6915

ER -