Toxicity and transformation of graphene oxide and reduced graphene oxide in bacteria biofilm

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Toxicity and transformation of graphene oxide and reduced graphene oxide in bacteria biofilm. / Guo, Zhiling; Xie, Changjian; Zhang, Peng; Zhang, Junzhe; Wang, Guohua; He, Xiao; Ma, Yuhui; Zhao, Bin; Zhang, Zhiyong.

In: Science of the Total Environment, 15.02.2017, p. 1300-1308.

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Guo, Zhiling ; Xie, Changjian ; Zhang, Peng ; Zhang, Junzhe ; Wang, Guohua ; He, Xiao ; Ma, Yuhui ; Zhao, Bin ; Zhang, Zhiyong. / Toxicity and transformation of graphene oxide and reduced graphene oxide in bacteria biofilm. In: Science of the Total Environment. 2017 ; pp. 1300-1308.

Bibtex

@article{80e842b456a74a4daef997a122a7c663,
title = "Toxicity and transformation of graphene oxide and reduced graphene oxide in bacteria biofilm",
abstract = "Impact of graphene based material (GNMs) on bacteria biofilm has not been well understood yet. In this study, we compared the impact of graphene oxide (GO) and reduced graphene oxide (rGO) on biofilm formation and development in Luria-Bertani (LB) medium using Escherichia coli and Staphylococcus aureus as models. GO significantly enhanced the cell growth, biofilm formation, and biofilm development even up to a concentration of 500 mg/L. In contrast, rGO (≥ 50 mg/L) strongly inhibited cell growth and biofilm formation. However, the inhibitory effects of rGO (50 mg/L and 100 mg/L) were attenuated in the mature phase (> 24 h) and eliminated at 48 h. GO at 250 mg/L decreased the reactive oxygen species (ROS) levels in biofilm and extracellular region at mature phase. ROS levels were significantly increased by rGO at early phase, while they returned to the same levels as control at mature phase. These results suggest that oxidative stress contributed to the inhibitory effect of rGO on bacterial biofilm. We further found that supplement of extracellular polymeric substances (EPS) in the growth medium attenuated the inhibitory effect of rGO on the growth of developed biofilm. XPS results showed that rGO were oxidized to GO which can enhance the bacterial growth. We deduced that the elimination of the toxicity of rGO at mature phase was contributed by EPS protection and the oxidation of rGO. This study provides new insights into the interaction of GNMs with bacteria biofilm.",
keywords = "graphene oxide, reduced graphene oxide, biofilm, toxicity, transformation",
author = "Zhiling Guo and Changjian Xie and Peng Zhang and Junzhe Zhang and Guohua Wang and Xiao He and Yuhui Ma and Bin Zhao and Zhiyong Zhang",
year = "2017",
month = feb,
day = "15",
doi = "10.1016/j.scitotenv.2016.12.093",
language = "English",
pages = "1300--1308",
journal = "Science of the Total Environment",
issn = "0048-9697",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Toxicity and transformation of graphene oxide and reduced graphene oxide in bacteria biofilm

AU - Guo, Zhiling

AU - Xie, Changjian

AU - Zhang, Peng

AU - Zhang, Junzhe

AU - Wang, Guohua

AU - He, Xiao

AU - Ma, Yuhui

AU - Zhao, Bin

AU - Zhang, Zhiyong

PY - 2017/2/15

Y1 - 2017/2/15

N2 - Impact of graphene based material (GNMs) on bacteria biofilm has not been well understood yet. In this study, we compared the impact of graphene oxide (GO) and reduced graphene oxide (rGO) on biofilm formation and development in Luria-Bertani (LB) medium using Escherichia coli and Staphylococcus aureus as models. GO significantly enhanced the cell growth, biofilm formation, and biofilm development even up to a concentration of 500 mg/L. In contrast, rGO (≥ 50 mg/L) strongly inhibited cell growth and biofilm formation. However, the inhibitory effects of rGO (50 mg/L and 100 mg/L) were attenuated in the mature phase (> 24 h) and eliminated at 48 h. GO at 250 mg/L decreased the reactive oxygen species (ROS) levels in biofilm and extracellular region at mature phase. ROS levels were significantly increased by rGO at early phase, while they returned to the same levels as control at mature phase. These results suggest that oxidative stress contributed to the inhibitory effect of rGO on bacterial biofilm. We further found that supplement of extracellular polymeric substances (EPS) in the growth medium attenuated the inhibitory effect of rGO on the growth of developed biofilm. XPS results showed that rGO were oxidized to GO which can enhance the bacterial growth. We deduced that the elimination of the toxicity of rGO at mature phase was contributed by EPS protection and the oxidation of rGO. This study provides new insights into the interaction of GNMs with bacteria biofilm.

AB - Impact of graphene based material (GNMs) on bacteria biofilm has not been well understood yet. In this study, we compared the impact of graphene oxide (GO) and reduced graphene oxide (rGO) on biofilm formation and development in Luria-Bertani (LB) medium using Escherichia coli and Staphylococcus aureus as models. GO significantly enhanced the cell growth, biofilm formation, and biofilm development even up to a concentration of 500 mg/L. In contrast, rGO (≥ 50 mg/L) strongly inhibited cell growth and biofilm formation. However, the inhibitory effects of rGO (50 mg/L and 100 mg/L) were attenuated in the mature phase (> 24 h) and eliminated at 48 h. GO at 250 mg/L decreased the reactive oxygen species (ROS) levels in biofilm and extracellular region at mature phase. ROS levels were significantly increased by rGO at early phase, while they returned to the same levels as control at mature phase. These results suggest that oxidative stress contributed to the inhibitory effect of rGO on bacterial biofilm. We further found that supplement of extracellular polymeric substances (EPS) in the growth medium attenuated the inhibitory effect of rGO on the growth of developed biofilm. XPS results showed that rGO were oxidized to GO which can enhance the bacterial growth. We deduced that the elimination of the toxicity of rGO at mature phase was contributed by EPS protection and the oxidation of rGO. This study provides new insights into the interaction of GNMs with bacteria biofilm.

KW - graphene oxide

KW - reduced graphene oxide

KW - biofilm

KW - toxicity

KW - transformation

U2 - 10.1016/j.scitotenv.2016.12.093

DO - 10.1016/j.scitotenv.2016.12.093

M3 - Article

SP - 1300

EP - 1308

JO - Science of the Total Environment

JF - Science of the Total Environment

SN - 0048-9697

ER -