Tissue Response and Immunoexpression of Interleukin 6 Promoted by Tricalcium Silicate-based Repair Materials after Subcutaneous Implantation in Rats

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Tissue Response and Immunoexpression of Interleukin 6 Promoted by Tricalcium Silicate-based Repair Materials after Subcutaneous Implantation in Rats. / Andrade, Aline S.; Silva, Guilherme F.; Camilleri, Josette; Cerri, Estela S.; Guerreiro-Tanomaru, Juliane M.; Cerri, Paulo S.; Tanomaru-Filho, Mário.

In: Journal of Endodontics, Vol. 44, No. 3, 03.2018, p. 458-463.

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Andrade, Aline S. ; Silva, Guilherme F. ; Camilleri, Josette ; Cerri, Estela S. ; Guerreiro-Tanomaru, Juliane M. ; Cerri, Paulo S. ; Tanomaru-Filho, Mário. / Tissue Response and Immunoexpression of Interleukin 6 Promoted by Tricalcium Silicate-based Repair Materials after Subcutaneous Implantation in Rats. In: Journal of Endodontics. 2018 ; Vol. 44, No. 3. pp. 458-463.

Bibtex

@article{cf571f081c784ffc8e9788115b087c20,
title = "Tissue Response and Immunoexpression of Interleukin 6 Promoted by Tricalcium Silicate-based Repair Materials after Subcutaneous Implantation in Rats",
abstract = "INTRODUCTION: The aim of the present study was to evaluate the inflammatory response induced by experimental tricalcium silicate cement with 20% zirconium oxide (TSC) and MTA Plus (MTAP; Avalon Biomed Inc, Bradenton, FL) in rat subcutaneous tissues.METHODS: Polyethylene tubes were filled with TSC (n = 20) and MTAP (n = 20) and implanted in the dorsal subcutaneous tissues of 32 rats. Empty tubes were used as the control (control group [CG], n = 20). After 7, 15, 30, and 60 days, the tubes with connective tissue were removed, and the inflammatory cells and immunolabeled cells for interleukin 6 (IL-6) were counted. Data were statistically analyzed using analysis of variance and the Tukey test (P ≤ .05).RESULTS: An increased number of inflammatory and immunolabeled cells for IL-6 were observed at 7 days. The number of inflammatory cells was higher for TSC and MTAP than the CG (P < .001) at 7 days; after 30 and 60 days, no significant differences were observed among the TSC, MTAP, and CG (P = .955). The number of immunolabeled cells for IL-6 was similar for TSC, MTAP, and CG at all evaluated periods. A gradual and significant decrease was observed in the number of inflammatory cells and IL-6-immunopositive cells. At 60 days, the capsules adjacent to TSC and MTAP exhibited fibroblasts and bundles of collagen fibers.CONCLUSIONS: TSC and MTAP caused a similar subcutaneous reaction in rats, suggesting that they are biocompatible and present similar immune responses.",
keywords = "zirconium oxide, biocompatibility , immunohistochemistry, interleuklin 6, tricalcium silicate ",
author = "Andrade, {Aline S.} and Silva, {Guilherme F.} and Josette Camilleri and Cerri, {Estela S.} and Guerreiro-Tanomaru, {Juliane M.} and Cerri, {Paulo S.} and M{\'a}rio Tanomaru-Filho",
note = "Copyright {\textcopyright} 2017 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.",
year = "2018",
month = mar,
doi = "10.1016/j.joen.2017.12.006",
language = "English",
volume = "44",
pages = "458--463",
journal = "Journal of Endodontics",
issn = "0099-2399",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Tissue Response and Immunoexpression of Interleukin 6 Promoted by Tricalcium Silicate-based Repair Materials after Subcutaneous Implantation in Rats

AU - Andrade, Aline S.

AU - Silva, Guilherme F.

AU - Camilleri, Josette

AU - Cerri, Estela S.

AU - Guerreiro-Tanomaru, Juliane M.

AU - Cerri, Paulo S.

AU - Tanomaru-Filho, Mário

N1 - Copyright © 2017 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

PY - 2018/3

Y1 - 2018/3

N2 - INTRODUCTION: The aim of the present study was to evaluate the inflammatory response induced by experimental tricalcium silicate cement with 20% zirconium oxide (TSC) and MTA Plus (MTAP; Avalon Biomed Inc, Bradenton, FL) in rat subcutaneous tissues.METHODS: Polyethylene tubes were filled with TSC (n = 20) and MTAP (n = 20) and implanted in the dorsal subcutaneous tissues of 32 rats. Empty tubes were used as the control (control group [CG], n = 20). After 7, 15, 30, and 60 days, the tubes with connective tissue were removed, and the inflammatory cells and immunolabeled cells for interleukin 6 (IL-6) were counted. Data were statistically analyzed using analysis of variance and the Tukey test (P ≤ .05).RESULTS: An increased number of inflammatory and immunolabeled cells for IL-6 were observed at 7 days. The number of inflammatory cells was higher for TSC and MTAP than the CG (P < .001) at 7 days; after 30 and 60 days, no significant differences were observed among the TSC, MTAP, and CG (P = .955). The number of immunolabeled cells for IL-6 was similar for TSC, MTAP, and CG at all evaluated periods. A gradual and significant decrease was observed in the number of inflammatory cells and IL-6-immunopositive cells. At 60 days, the capsules adjacent to TSC and MTAP exhibited fibroblasts and bundles of collagen fibers.CONCLUSIONS: TSC and MTAP caused a similar subcutaneous reaction in rats, suggesting that they are biocompatible and present similar immune responses.

AB - INTRODUCTION: The aim of the present study was to evaluate the inflammatory response induced by experimental tricalcium silicate cement with 20% zirconium oxide (TSC) and MTA Plus (MTAP; Avalon Biomed Inc, Bradenton, FL) in rat subcutaneous tissues.METHODS: Polyethylene tubes were filled with TSC (n = 20) and MTAP (n = 20) and implanted in the dorsal subcutaneous tissues of 32 rats. Empty tubes were used as the control (control group [CG], n = 20). After 7, 15, 30, and 60 days, the tubes with connective tissue were removed, and the inflammatory cells and immunolabeled cells for interleukin 6 (IL-6) were counted. Data were statistically analyzed using analysis of variance and the Tukey test (P ≤ .05).RESULTS: An increased number of inflammatory and immunolabeled cells for IL-6 were observed at 7 days. The number of inflammatory cells was higher for TSC and MTAP than the CG (P < .001) at 7 days; after 30 and 60 days, no significant differences were observed among the TSC, MTAP, and CG (P = .955). The number of immunolabeled cells for IL-6 was similar for TSC, MTAP, and CG at all evaluated periods. A gradual and significant decrease was observed in the number of inflammatory cells and IL-6-immunopositive cells. At 60 days, the capsules adjacent to TSC and MTAP exhibited fibroblasts and bundles of collagen fibers.CONCLUSIONS: TSC and MTAP caused a similar subcutaneous reaction in rats, suggesting that they are biocompatible and present similar immune responses.

KW - zirconium oxide

KW - biocompatibility

KW - immunohistochemistry

KW - interleuklin 6

KW - tricalcium silicate

U2 - 10.1016/j.joen.2017.12.006

DO - 10.1016/j.joen.2017.12.006

M3 - Article

C2 - 29398089

VL - 44

SP - 458

EP - 463

JO - Journal of Endodontics

JF - Journal of Endodontics

SN - 0099-2399

IS - 3

ER -