Tissue Response and Immunoexpression of Interleukin 6 Promoted by Tricalcium Silicate-based Repair Materials after Subcutaneous Implantation in Rats

Research output: Contribution to journalArticlepeer-review


  • Aline S. Andrade
  • Guilherme F. Silva
  • Estela S. Cerri
  • Juliane M. Guerreiro-Tanomaru
  • Paulo S. Cerri
  • Mário Tanomaru-Filho

Colleges, School and Institutes

External organisations

  • Department of Restorative Dentistry, Araraquara Dental School, UNESP–São Paulo State University
  • Department of Morphology, Araraquara Dental School, UNESP–São Paulo State University


INTRODUCTION: The aim of the present study was to evaluate the inflammatory response induced by experimental tricalcium silicate cement with 20% zirconium oxide (TSC) and MTA Plus (MTAP; Avalon Biomed Inc, Bradenton, FL) in rat subcutaneous tissues.

METHODS: Polyethylene tubes were filled with TSC (n = 20) and MTAP (n = 20) and implanted in the dorsal subcutaneous tissues of 32 rats. Empty tubes were used as the control (control group [CG], n = 20). After 7, 15, 30, and 60 days, the tubes with connective tissue were removed, and the inflammatory cells and immunolabeled cells for interleukin 6 (IL-6) were counted. Data were statistically analyzed using analysis of variance and the Tukey test (P ≤ .05).

RESULTS: An increased number of inflammatory and immunolabeled cells for IL-6 were observed at 7 days. The number of inflammatory cells was higher for TSC and MTAP than the CG (P < .001) at 7 days; after 30 and 60 days, no significant differences were observed among the TSC, MTAP, and CG (P = .955). The number of immunolabeled cells for IL-6 was similar for TSC, MTAP, and CG at all evaluated periods. A gradual and significant decrease was observed in the number of inflammatory cells and IL-6-immunopositive cells. At 60 days, the capsules adjacent to TSC and MTAP exhibited fibroblasts and bundles of collagen fibers.

CONCLUSIONS: TSC and MTAP caused a similar subcutaneous reaction in rats, suggesting that they are biocompatible and present similar immune responses.


Original languageEnglish
Pages (from-to)458-463
Number of pages6
JournalJournal of Endodontics
Issue number3
Early online date21 Feb 2018
Publication statusPublished - Mar 2018


  • zirconium oxide, biocompatibility , immunohistochemistry, interleuklin 6, tricalcium silicate