TY - JOUR
T1 - Three-Dimensional Cotton-Wool-like Polyhydroxybutyrate/Siloxane-doped Vaterite Composite Fibrous Scaffolds: Effect of Imogolite-coating on Physicochemical and Cell Adhesion Properties
AU - Obata, Akiko
AU - Mori, Kazuma
AU - Inukai, Keiichi
AU - Kato, Katsuya
AU - Poologasundarampillai, Gowsihan
AU - Kasuga, Toshihiro
PY - 2020/2/19
Y1 - 2020/2/19
N2 - Poly(3-hydroxybutyrate-co-4-hydroxybutyrate), P(3HB-4HB), and siloxane-doped vaterite (SiV) composite fibrous scaffolds with 3D cotton-wool-like structure were developed using an electrospinning system for use in bone tissue regeneration. Scaffolds exhibited a significantly larger fiber-fiber separation distribution than non-woven fiber mats as observed with micro-computed tomographic studies. Coating the hydrophobic P(3HB-4HB)/SiV fibers with imogolite nanotubes (INT), aluminum silicate nanotubes, made the 3D construct hydrophilic and improved water penetration into the 3D structure (~2 s). Coating efficacy was confirmed by the detection of aluminum on the surface of fibers using scanning electron microscopy (SEM) energy dispersive spectroscopy (EDS). Dissolution experiments showed increased release of silicate ions in cell culture medium which can improve migration and mineralization of osteogenic cells inside of the 3D structure. The coating also contributed to an enhanced adhesion and migration of osteoblast-like cells (SaOS-2) within the 3D construct. The differentiation and mineralization of the cells were not affected by the coating. The coating for such cotton-wool-like structured scaffolds was effective for an enhancement of cell functions on early stages of culture. Thus, the developed materials with 3D structure, flexibility, silicate-ion release ability, and cell compatibility are expected to be good candidate materials for bone tissue regeneration.
AB - Poly(3-hydroxybutyrate-co-4-hydroxybutyrate), P(3HB-4HB), and siloxane-doped vaterite (SiV) composite fibrous scaffolds with 3D cotton-wool-like structure were developed using an electrospinning system for use in bone tissue regeneration. Scaffolds exhibited a significantly larger fiber-fiber separation distribution than non-woven fiber mats as observed with micro-computed tomographic studies. Coating the hydrophobic P(3HB-4HB)/SiV fibers with imogolite nanotubes (INT), aluminum silicate nanotubes, made the 3D construct hydrophilic and improved water penetration into the 3D structure (~2 s). Coating efficacy was confirmed by the detection of aluminum on the surface of fibers using scanning electron microscopy (SEM) energy dispersive spectroscopy (EDS). Dissolution experiments showed increased release of silicate ions in cell culture medium which can improve migration and mineralization of osteogenic cells inside of the 3D structure. The coating also contributed to an enhanced adhesion and migration of osteoblast-like cells (SaOS-2) within the 3D construct. The differentiation and mineralization of the cells were not affected by the coating. The coating for such cotton-wool-like structured scaffolds was effective for an enhancement of cell functions on early stages of culture. Thus, the developed materials with 3D structure, flexibility, silicate-ion release ability, and cell compatibility are expected to be good candidate materials for bone tissue regeneration.
KW - 3D μCT quantification
KW - cell adhesion
KW - cotton-wool like structure
KW - electrospinning
KW - imogolite
KW - polyhydroxyalkanoates
UR - http://www.scopus.com/inward/record.url?scp=85081217353&partnerID=8YFLogxK
U2 - 10.3389/fmats.2020.00033
DO - 10.3389/fmats.2020.00033
M3 - Article
SN - 2296-8016
VL - 7
JO - Frontiers in Materials
JF - Frontiers in Materials
M1 - 33
ER -