Three Dimensional Auto-Alignment of the ICSI Pipette

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Three Dimensional Auto-Alignment of the ICSI Pipette. / Sadak, Ferhat; Saadat, Mozafar; Hajiyavand, Amir M.

In: IEEE Access, Vol. 7, 8746994, 2019, p. 99360-99370.

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@article{92becc0739f64b1eb1c131f8a8890dc2,
title = "Three Dimensional Auto-Alignment of the ICSI Pipette",
abstract = "Intracytoplasmic sperm injection (ICSI) is an assisted reproductive technology used in infertility treatment, where a single sperm is selected and immobilized using a glass injection pipette and is inserted directly into the cytoplasm of an oocyte under the microscope. Auto-alignment of the injection pipette is a prerequisite for any proposed automated oocyte injection procedure. In this paper, an auto-alignment procedure has been proposed. This technique requires the positioning of the injection and holding pipettes in the three orthogonal axes under microscopy which is complex. The existing proposed methods are system-specific and require appropriate algorithms. In this paper, 12 commonly used focusing algorithms were evaluated to verify the optimal one for the ICSI application. These algorithms were assessed by measuring focusing accuracy, range, the number of false maxima, and the width of the curve. The focus level for each pipette is calculated by the algorithm using focus measure functions (FMA). The Fibonacci search algorithm is employed for controlling the z-axis of the motorized stage to obtain the focal plane of the injection and holding pipette. The experimental results verified that the Brenner gradient has demonstrated the highest overall performance for injection pipette focalization, while the energy of gradient has presented a highest overall performance for holding pipette focalization. ",
keywords = "autofocusing, computer vision, Fibonacci search, focus measurement algorithm, Intracytoplasmic sperm injection (ICSI)",
author = "Ferhat Sadak and Mozafar Saadat and Hajiyavand, {Amir M.}",
note = "Funding Information: This work was supported in part by the U.K. Medical Research Council (MRC). Funding Information: The authors would like to thank UK Medical Research Council (MRC) for their support of aspects of this work. The authors also wish to thank Basler AG for their contribution in the vision development part of this work. Publisher Copyright: {\textcopyright} 2013 IEEE. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2019",
doi = "10.1109/ACCESS.2019.2925465",
language = "English",
volume = "7",
pages = "99360--99370",
journal = "IEEE Access",
issn = "2169-3536",
publisher = "IEEE Xplore",

}

RIS

TY - JOUR

T1 - Three Dimensional Auto-Alignment of the ICSI Pipette

AU - Sadak, Ferhat

AU - Saadat, Mozafar

AU - Hajiyavand, Amir M.

N1 - Funding Information: This work was supported in part by the U.K. Medical Research Council (MRC). Funding Information: The authors would like to thank UK Medical Research Council (MRC) for their support of aspects of this work. The authors also wish to thank Basler AG for their contribution in the vision development part of this work. Publisher Copyright: © 2013 IEEE. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2019

Y1 - 2019

N2 - Intracytoplasmic sperm injection (ICSI) is an assisted reproductive technology used in infertility treatment, where a single sperm is selected and immobilized using a glass injection pipette and is inserted directly into the cytoplasm of an oocyte under the microscope. Auto-alignment of the injection pipette is a prerequisite for any proposed automated oocyte injection procedure. In this paper, an auto-alignment procedure has been proposed. This technique requires the positioning of the injection and holding pipettes in the three orthogonal axes under microscopy which is complex. The existing proposed methods are system-specific and require appropriate algorithms. In this paper, 12 commonly used focusing algorithms were evaluated to verify the optimal one for the ICSI application. These algorithms were assessed by measuring focusing accuracy, range, the number of false maxima, and the width of the curve. The focus level for each pipette is calculated by the algorithm using focus measure functions (FMA). The Fibonacci search algorithm is employed for controlling the z-axis of the motorized stage to obtain the focal plane of the injection and holding pipette. The experimental results verified that the Brenner gradient has demonstrated the highest overall performance for injection pipette focalization, while the energy of gradient has presented a highest overall performance for holding pipette focalization.

AB - Intracytoplasmic sperm injection (ICSI) is an assisted reproductive technology used in infertility treatment, where a single sperm is selected and immobilized using a glass injection pipette and is inserted directly into the cytoplasm of an oocyte under the microscope. Auto-alignment of the injection pipette is a prerequisite for any proposed automated oocyte injection procedure. In this paper, an auto-alignment procedure has been proposed. This technique requires the positioning of the injection and holding pipettes in the three orthogonal axes under microscopy which is complex. The existing proposed methods are system-specific and require appropriate algorithms. In this paper, 12 commonly used focusing algorithms were evaluated to verify the optimal one for the ICSI application. These algorithms were assessed by measuring focusing accuracy, range, the number of false maxima, and the width of the curve. The focus level for each pipette is calculated by the algorithm using focus measure functions (FMA). The Fibonacci search algorithm is employed for controlling the z-axis of the motorized stage to obtain the focal plane of the injection and holding pipette. The experimental results verified that the Brenner gradient has demonstrated the highest overall performance for injection pipette focalization, while the energy of gradient has presented a highest overall performance for holding pipette focalization.

KW - autofocusing

KW - computer vision

KW - Fibonacci search

KW - focus measurement algorithm

KW - Intracytoplasmic sperm injection (ICSI)

UR - http://www.scopus.com/inward/record.url?scp=85075320264&partnerID=8YFLogxK

U2 - 10.1109/ACCESS.2019.2925465

DO - 10.1109/ACCESS.2019.2925465

M3 - Article

AN - SCOPUS:85075320264

VL - 7

SP - 99360

EP - 99370

JO - IEEE Access

JF - IEEE Access

SN - 2169-3536

M1 - 8746994

ER -