Thiamethoxam exposure deregulates short ORF gene expression in the honey bee and compromises immune response to bacteria

Research output: Contribution to journalArticlepeer-review

Standard

Thiamethoxam exposure deregulates short ORF gene expression in the honey bee and compromises immune response to bacteria. / Decio, Pamela; Ustaoglu, Pinar; Derecka, Kamila ; Hardy, Ian C. W. ; Roat, Thaisa C. ; Malaspina, Osmar; Mongan, Nigel P.; Stoger, Reinhard; Soller, Matthias.

In: Scientific Reports, Vol. 11, No. 1, 1489 , 15.01.2021.

Research output: Contribution to journalArticlepeer-review

Harvard

APA

Vancouver

Author

Decio, Pamela ; Ustaoglu, Pinar ; Derecka, Kamila ; Hardy, Ian C. W. ; Roat, Thaisa C. ; Malaspina, Osmar ; Mongan, Nigel P. ; Stoger, Reinhard ; Soller, Matthias. / Thiamethoxam exposure deregulates short ORF gene expression in the honey bee and compromises immune response to bacteria. In: Scientific Reports. 2021 ; Vol. 11, No. 1.

Bibtex

@article{94fa721baf6e488fa3ec98dc4b841b8f,
title = "Thiamethoxam exposure deregulates short ORF gene expression in the honey bee and compromises immune response to bacteria",
abstract = "Maximizing crop yields relies on the use of agrochemicals to control insect pests. One of the most widely used classes of insecticides are neonicotinoids that interfere with signalling of the neurotransmitter acetylcholine, but these can also disrupt crop-pollination services provided by bees. Here, we analysed whether chronic low dose long-term exposure to the neonicotinoid thiamethoxam alters gene expression and alternative splicing in brains of Africanized honey bees, Apis mellifera, as adaptation to altered neuronal signalling. We find differentially regulated genes that show concentration-dependent responses to thiamethoxam, but no changes in alternative splicing. Most differentially expressed genes have no annotated function but encode short Open Reading Frames, a characteristic feature of anti-microbial peptides. As this suggested that immune responses may be compromised by thiamethoxam exposure, we tested the impact of thiamethoxam on bee immunity by injecting bacteria. We show that intrinsically sub-lethal thiamethoxam exposure makes bees more vulnerable to normally non-pathogenic bacteria. Our findings imply a synergistic mechanism for the observed bee population declines that concern agriculturists, conservation ecologists and the public.",
author = "Pamela Decio and Pinar Ustaoglu and Kamila Derecka and Hardy, {Ian C. W.} and Roat, {Thaisa C.} and Osmar Malaspina and Mongan, {Nigel P.} and Reinhard Stoger and Matthias Soller",
note = "Funding Information: We thank N. Parker and the Winterbourne garden for bees, N. Parker for bee suits, D. Scocchia, V. Soller-Haussmann and K. Nallasivan for help with bee collections. G. Salmond for bacterial strains, D. Scocchia for help with bacterial culturing and genotyping, and L. Orsini, E. Davies and I. Haussmann for comments on the manuscript. For this work we acknowledge funding from the Foundation for Research Support of the State of S{\~a}o Paulo, FAPESP (2012/13370-8; 2013/07251-9; 2014/23197-7; 2015/22368-5), the Biotechnology and Biological Sciences Research Council (BBSRC), the Nottingham-Birmingham Fund, and the Sukran Sinan Memory Fund.",
year = "2021",
month = jan,
day = "15",
doi = "10.1038/s41598-020-80620-7",
language = "English",
volume = "11",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - Thiamethoxam exposure deregulates short ORF gene expression in the honey bee and compromises immune response to bacteria

AU - Decio, Pamela

AU - Ustaoglu, Pinar

AU - Derecka, Kamila

AU - Hardy, Ian C. W.

AU - Roat, Thaisa C.

AU - Malaspina, Osmar

AU - Mongan, Nigel P.

AU - Stoger, Reinhard

AU - Soller, Matthias

N1 - Funding Information: We thank N. Parker and the Winterbourne garden for bees, N. Parker for bee suits, D. Scocchia, V. Soller-Haussmann and K. Nallasivan for help with bee collections. G. Salmond for bacterial strains, D. Scocchia for help with bacterial culturing and genotyping, and L. Orsini, E. Davies and I. Haussmann for comments on the manuscript. For this work we acknowledge funding from the Foundation for Research Support of the State of São Paulo, FAPESP (2012/13370-8; 2013/07251-9; 2014/23197-7; 2015/22368-5), the Biotechnology and Biological Sciences Research Council (BBSRC), the Nottingham-Birmingham Fund, and the Sukran Sinan Memory Fund.

PY - 2021/1/15

Y1 - 2021/1/15

N2 - Maximizing crop yields relies on the use of agrochemicals to control insect pests. One of the most widely used classes of insecticides are neonicotinoids that interfere with signalling of the neurotransmitter acetylcholine, but these can also disrupt crop-pollination services provided by bees. Here, we analysed whether chronic low dose long-term exposure to the neonicotinoid thiamethoxam alters gene expression and alternative splicing in brains of Africanized honey bees, Apis mellifera, as adaptation to altered neuronal signalling. We find differentially regulated genes that show concentration-dependent responses to thiamethoxam, but no changes in alternative splicing. Most differentially expressed genes have no annotated function but encode short Open Reading Frames, a characteristic feature of anti-microbial peptides. As this suggested that immune responses may be compromised by thiamethoxam exposure, we tested the impact of thiamethoxam on bee immunity by injecting bacteria. We show that intrinsically sub-lethal thiamethoxam exposure makes bees more vulnerable to normally non-pathogenic bacteria. Our findings imply a synergistic mechanism for the observed bee population declines that concern agriculturists, conservation ecologists and the public.

AB - Maximizing crop yields relies on the use of agrochemicals to control insect pests. One of the most widely used classes of insecticides are neonicotinoids that interfere with signalling of the neurotransmitter acetylcholine, but these can also disrupt crop-pollination services provided by bees. Here, we analysed whether chronic low dose long-term exposure to the neonicotinoid thiamethoxam alters gene expression and alternative splicing in brains of Africanized honey bees, Apis mellifera, as adaptation to altered neuronal signalling. We find differentially regulated genes that show concentration-dependent responses to thiamethoxam, but no changes in alternative splicing. Most differentially expressed genes have no annotated function but encode short Open Reading Frames, a characteristic feature of anti-microbial peptides. As this suggested that immune responses may be compromised by thiamethoxam exposure, we tested the impact of thiamethoxam on bee immunity by injecting bacteria. We show that intrinsically sub-lethal thiamethoxam exposure makes bees more vulnerable to normally non-pathogenic bacteria. Our findings imply a synergistic mechanism for the observed bee population declines that concern agriculturists, conservation ecologists and the public.

UR - http://www.scopus.com/inward/record.url?scp=85100076354&partnerID=8YFLogxK

U2 - 10.1038/s41598-020-80620-7

DO - 10.1038/s41598-020-80620-7

M3 - Article

C2 - 33452318

VL - 11

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 1489

ER -