The utility of ultra-high performance supercritical fluid chromatography–tandem mass spectrometry (UHPSFC-MS/MS) for clinically relevant steroid analysis

Research output: Contribution to journalArticle

Abstract

Liquid chromatography tandem mass spectrometry (LC-MS/MS) assays are considered the reference standard for serum steroid hormone analyses, while full urinary steroid profiles are only achievable by gas chromatography (GC–MS). Both LC-MS/MS and GC–MS have well documented strengths and limitations. Recently, commercial ultra-high performance supercritical fluid chromatography–tandem mass spectrometry (UHPSFC-MS/MS) systems have been developed. These systems combine the resolution of GC with the high-throughput capabilities of UHPLC. Uptake of this new technology into research and clinical labs has been slow, possibly due to the perceived increase in complexity. Here we therefore present fundamental principles of UHPSFC-MS/MS and the likely applications for this technology in the clinical research setting, while commenting on potential hurdles based on our experience to date.

Details

Original languageEnglish
Pages (from-to)36-41
JournalJournal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Volume1085
Early online date20 Mar 2018
Publication statusPublished - 15 May 2018

Keywords

  • Ultra-high performance supercritical fluid chromatography–tandem mass spectrometry , Ultra-high performance liquid chromatography–tandem mass spectrometry , Gas chromatography–mass spectrometry , steroid analysis