The utility of ultra-high performance supercritical fluid chromatography–tandem mass spectrometry (UHPSFC-MS/MS) for clinically relevant steroid analysis
Research output: Contribution to journal › Article › peer-review
Liquid chromatography tandem mass spectrometry (LC-MS/MS) assays are considered the reference standard for serum steroid hormone analyses, while full urinary steroid profiles are only achievable by gas chromatography (GC–MS). Both LC-MS/MS and GC–MS have well documented strengths and limitations. Recently, commercial ultra-high performance supercritical fluid chromatography–tandem mass spectrometry (UHPSFC-MS/MS) systems have been developed. These systems combine the resolution of GC with the high-throughput capabilities of UHPLC. Uptake of this new technology into research and clinical labs has been slow, possibly due to the perceived increase in complexity. Here we therefore present fundamental principles of UHPSFC-MS/MS and the likely applications for this technology in the clinical research setting, while commenting on potential hurdles based on our experience to date.
|Journal||Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences|
|Early online date||20 Mar 2018|
|Publication status||Published - 15 May 2018|
- Ultra-high performance supercritical fluid chromatography–tandem mass spectrometry , Ultra-high performance liquid chromatography–tandem mass spectrometry , Gas chromatography–mass spectrometry , steroid analysis