The use of site-directed mutagenesis to study GPCRs

Research output: Contribution to journalArticlepeer-review

Authors

Colleges, School and Institutes

Abstract

G protein coupled receptors (GPCRs) are highly flexible and dynamic proteins, which are able to interact with diverse ligands, effectors, and regulatory proteins. Site-directed mutagenesis (SDM) is a powerful tool for providing insight into how these proteins actually work, both in its own right and when used in conjunction with information provided by other techniques such as crystallography or molecular modelling. Mutagenesis has been used to identify and characterise a myriad of functionally important residues, motifs and domains within the GPCR architecture, and to identify aspects of similarity and differences between the major families of GPCRs. This chapter presents the necessary information for undertaking informative SDM of these proteins. Whilst this is relevant to protein structure/function studies in -general, specific pitfalls and protocols suited to investigating GPCRs in particular will be highlighted.

Details

Original languageEnglish
Pages (from-to)85-98
Number of pages14
JournalMethods in molecular biology
Volume746
Publication statusPublished - 2011

Keywords

  • Cells, Cultured, Genetic Vectors, Mutagenesis, Site-Directed, Mutant Chimeric Proteins, Receptors, G-Protein-Coupled, Transfection