TY - JOUR
T1 - The unusual extended signal peptide region is not required for secretion and function of an Escherichia coli autotransporter.
AU - Leyton, Denisse
AU - de Luna, MG
AU - Sevastsyanovich, Yanina
AU - Tveen Jensen, Karina
AU - Browning, Douglas
AU - Scott-Tucker, A
AU - Henderson, Ian
PY - 2010/10/1
Y1 - 2010/10/1
N2 - The plasmid-encoded toxin, Pet, a prototypical member of the serine protease autotransporters of the Enterobacteriaceae, possesses an unusually long signal peptide, which can be divided into five regions termed N1 (charged), H1 (hydrophobic), N2, H2 and C (cleavage site) domains. The N1 and H1 regions correspond to a conserved N-terminal extension previously designated the extended signal peptide region (ESPR), while the N2, H2 and C regions resemble typical Sec-dependent signal sequences and exhibit considerable sequence variability. We have shown previously that the ESPR directs Sec-dependent, post-translational translocation of Pet across the bacterial inner membrane. In this study, we demonstrate that the ESPR is not essential for the secretion or the function of Pet.
AB - The plasmid-encoded toxin, Pet, a prototypical member of the serine protease autotransporters of the Enterobacteriaceae, possesses an unusually long signal peptide, which can be divided into five regions termed N1 (charged), H1 (hydrophobic), N2, H2 and C (cleavage site) domains. The N1 and H1 regions correspond to a conserved N-terminal extension previously designated the extended signal peptide region (ESPR), while the N2, H2 and C regions resemble typical Sec-dependent signal sequences and exhibit considerable sequence variability. We have shown previously that the ESPR directs Sec-dependent, post-translational translocation of Pet across the bacterial inner membrane. In this study, we demonstrate that the ESPR is not essential for the secretion or the function of Pet.
KW - secretion
KW - Escherichia coli
KW - plasmid-encoded toxin (Pet)
KW - extended signal peptide region (ESPR)
KW - autotransporter
U2 - 10.1111/j.1574-6968.2010.02081.x
DO - 10.1111/j.1574-6968.2010.02081.x
M3 - Article
C2 - 20735484
SN - 1574-6968
SN - 1574-6968
SN - 1574-6968
SN - 1574-6968
SN - 1574-6968
SN - 1574-6968
SN - 1574-6968
SN - 1574-6968
SN - 1574-6968
SN - 1574-6968
SN - 1574-6968
SN - 1574-6968
SN - 1574-6968
SN - 1574-6968
SN - 1574-6968
VL - 311
SP - 133
EP - 139
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 2
ER -