The transcriptional program controlled by the stem cell leukemia gene Scl/Tal1 during early embryonic hematopoietic development

Research output: Contribution to journalArticle

Standard

The transcriptional program controlled by the stem cell leukemia gene Scl/Tal1 during early embryonic hematopoietic development. / Wilson, NK; Miranda-Saavedra, D; Kinston, S; Bonadies, N; Foster, SD; Calero-Nieto, F; Dawson, MA; Donaldson, IJ; Dumon, Stephanie; Frampton, Jonathan; Janky, R; Sun, XH; Teichmann, SA; Bannister, AJ; Goettgens, B.

In: Blood, Vol. 113, No. 22, 01.05.2009, p. 5456-5465.

Research output: Contribution to journalArticle

Harvard

Wilson, NK, Miranda-Saavedra, D, Kinston, S, Bonadies, N, Foster, SD, Calero-Nieto, F, Dawson, MA, Donaldson, IJ, Dumon, S, Frampton, J, Janky, R, Sun, XH, Teichmann, SA, Bannister, AJ & Goettgens, B 2009, 'The transcriptional program controlled by the stem cell leukemia gene Scl/Tal1 during early embryonic hematopoietic development', Blood, vol. 113, no. 22, pp. 5456-5465. https://doi.org/10.1182/blood-2009-01-200048

APA

Wilson, NK., Miranda-Saavedra, D., Kinston, S., Bonadies, N., Foster, SD., Calero-Nieto, F., Dawson, MA., Donaldson, IJ., Dumon, S., Frampton, J., Janky, R., Sun, XH., Teichmann, SA., Bannister, AJ., & Goettgens, B. (2009). The transcriptional program controlled by the stem cell leukemia gene Scl/Tal1 during early embryonic hematopoietic development. Blood, 113(22), 5456-5465. https://doi.org/10.1182/blood-2009-01-200048

Vancouver

Wilson NK, Miranda-Saavedra D, Kinston S, Bonadies N, Foster SD, Calero-Nieto F et al. The transcriptional program controlled by the stem cell leukemia gene Scl/Tal1 during early embryonic hematopoietic development. Blood. 2009 May 1;113(22):5456-5465. https://doi.org/10.1182/blood-2009-01-200048

Author

Wilson, NK ; Miranda-Saavedra, D ; Kinston, S ; Bonadies, N ; Foster, SD ; Calero-Nieto, F ; Dawson, MA ; Donaldson, IJ ; Dumon, Stephanie ; Frampton, Jonathan ; Janky, R ; Sun, XH ; Teichmann, SA ; Bannister, AJ ; Goettgens, B. / The transcriptional program controlled by the stem cell leukemia gene Scl/Tal1 during early embryonic hematopoietic development. In: Blood. 2009 ; Vol. 113, No. 22. pp. 5456-5465.

Bibtex

@article{0a4daf7fba984a09968446e73730046e,
title = "The transcriptional program controlled by the stem cell leukemia gene Scl/Tal1 during early embryonic hematopoietic development",
abstract = "The basic helix-loop-helix transcription factor Scl/Tal1 controls the development and subsequent differentiation of hematopoietic stem cells (HSCs). However, because few Scl target genes have been validated to date, the underlying mechanisms have remained largely unknown. In this study, we have used ChIP-Seq technology (coupling chromatin immunoprecipitation with deep sequencing) to generate a genome-wide catalog of Scl-binding events in a stem/progenitor cell line, followed by validation using primary fetal liver cells and comprehensive transgenic mouse assays. Transgenic analysis provided in vivo validation of multiple new direct Scl target genes and allowed us to reconstruct an in vivo validated network consisting of 17 factors and their respective regulatory elements. By coupling ChIP-Seq in model cell lines with in vivo transgenic validation and sophisticated bioinformatic analysis, we have identified a widely applicable strategy for the reconstruction of stem cell regulatory networks in which biologic material is otherwise limiting. Moreover, in addition to revealing multiple previously unrecognized links to known HSC regulators, as well as novel links to genes not previously implicated in HSC function, comprehensive transgenic analysis of regulatory elements provided substantial new insights into the transcriptional control of several important hematopoietic regulators, including Cbfa2t3h/Eto2, Cebpe, Nfe2, Zfpm1/Fog1, Erg, Mafk, Gfi1b, and Myb. (Blood. 2009; 113: 5456-5465)",
author = "NK Wilson and D Miranda-Saavedra and S Kinston and N Bonadies and SD Foster and F Calero-Nieto and MA Dawson and IJ Donaldson and Stephanie Dumon and Jonathan Frampton and R Janky and XH Sun and SA Teichmann and AJ Bannister and B Goettgens",
year = "2009",
month = may,
day = "1",
doi = "10.1182/blood-2009-01-200048",
language = "English",
volume = "113",
pages = "5456--5465",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "22",

}

RIS

TY - JOUR

T1 - The transcriptional program controlled by the stem cell leukemia gene Scl/Tal1 during early embryonic hematopoietic development

AU - Wilson, NK

AU - Miranda-Saavedra, D

AU - Kinston, S

AU - Bonadies, N

AU - Foster, SD

AU - Calero-Nieto, F

AU - Dawson, MA

AU - Donaldson, IJ

AU - Dumon, Stephanie

AU - Frampton, Jonathan

AU - Janky, R

AU - Sun, XH

AU - Teichmann, SA

AU - Bannister, AJ

AU - Goettgens, B

PY - 2009/5/1

Y1 - 2009/5/1

N2 - The basic helix-loop-helix transcription factor Scl/Tal1 controls the development and subsequent differentiation of hematopoietic stem cells (HSCs). However, because few Scl target genes have been validated to date, the underlying mechanisms have remained largely unknown. In this study, we have used ChIP-Seq technology (coupling chromatin immunoprecipitation with deep sequencing) to generate a genome-wide catalog of Scl-binding events in a stem/progenitor cell line, followed by validation using primary fetal liver cells and comprehensive transgenic mouse assays. Transgenic analysis provided in vivo validation of multiple new direct Scl target genes and allowed us to reconstruct an in vivo validated network consisting of 17 factors and their respective regulatory elements. By coupling ChIP-Seq in model cell lines with in vivo transgenic validation and sophisticated bioinformatic analysis, we have identified a widely applicable strategy for the reconstruction of stem cell regulatory networks in which biologic material is otherwise limiting. Moreover, in addition to revealing multiple previously unrecognized links to known HSC regulators, as well as novel links to genes not previously implicated in HSC function, comprehensive transgenic analysis of regulatory elements provided substantial new insights into the transcriptional control of several important hematopoietic regulators, including Cbfa2t3h/Eto2, Cebpe, Nfe2, Zfpm1/Fog1, Erg, Mafk, Gfi1b, and Myb. (Blood. 2009; 113: 5456-5465)

AB - The basic helix-loop-helix transcription factor Scl/Tal1 controls the development and subsequent differentiation of hematopoietic stem cells (HSCs). However, because few Scl target genes have been validated to date, the underlying mechanisms have remained largely unknown. In this study, we have used ChIP-Seq technology (coupling chromatin immunoprecipitation with deep sequencing) to generate a genome-wide catalog of Scl-binding events in a stem/progenitor cell line, followed by validation using primary fetal liver cells and comprehensive transgenic mouse assays. Transgenic analysis provided in vivo validation of multiple new direct Scl target genes and allowed us to reconstruct an in vivo validated network consisting of 17 factors and their respective regulatory elements. By coupling ChIP-Seq in model cell lines with in vivo transgenic validation and sophisticated bioinformatic analysis, we have identified a widely applicable strategy for the reconstruction of stem cell regulatory networks in which biologic material is otherwise limiting. Moreover, in addition to revealing multiple previously unrecognized links to known HSC regulators, as well as novel links to genes not previously implicated in HSC function, comprehensive transgenic analysis of regulatory elements provided substantial new insights into the transcriptional control of several important hematopoietic regulators, including Cbfa2t3h/Eto2, Cebpe, Nfe2, Zfpm1/Fog1, Erg, Mafk, Gfi1b, and Myb. (Blood. 2009; 113: 5456-5465)

U2 - 10.1182/blood-2009-01-200048

DO - 10.1182/blood-2009-01-200048

M3 - Article

C2 - 19346495

VL - 113

SP - 5456

EP - 5465

JO - Blood

JF - Blood

SN - 0006-4971

IS - 22

ER -