The RNA helicase UPF1 associates with mRNAs co-transcriptionally and is required for the release of mRNAs from gene loci

Research output: Contribution to journalArticlepeer-review

Standard

The RNA helicase UPF1 associates with mRNAs co-transcriptionally and is required for the release of mRNAs from gene loci. / Singh, Anand; Roy Choudhury, Subhendu; De, Sandip; Zhang, Jie; Kissane, Stephen; Dwivedi, Vibha; Ramanathan, Preethi; Petric, Marija; Orsini, Luisa; Hebenstreit, Daniel; Brogna, Saverio.

In: eLife, Vol. 8, No. 8, e41444, 25.03.2019.

Research output: Contribution to journalArticlepeer-review

Harvard

APA

Vancouver

Author

Singh, Anand ; Roy Choudhury, Subhendu ; De, Sandip ; Zhang, Jie ; Kissane, Stephen ; Dwivedi, Vibha ; Ramanathan, Preethi ; Petric, Marija ; Orsini, Luisa ; Hebenstreit, Daniel ; Brogna, Saverio. / The RNA helicase UPF1 associates with mRNAs co-transcriptionally and is required for the release of mRNAs from gene loci. In: eLife. 2019 ; Vol. 8, No. 8.

Bibtex

@article{599dbfde6f9745c8a6db9e7541f79ee0,
title = "The RNA helicase UPF1 associates with mRNAs co-transcriptionally and is required for the release of mRNAs from gene loci",
abstract = "UPF1 is an RNA helicase that is required for efficient nonsense-mediated mRNA decay (NMD) in eukaryotes, and the predominant view is that UPF1 mainly operates on the 3'UTRs of mRNAs that are directed for NMD in the cytoplasm. Here we offer evidence, obtained from Drosophila, that UPF1 constantly moves between the nucleus and cytoplasm by a mechanism that requires its RNA helicase activity. UPF1 is associated, genome-wide, with nascent RNAs at most of the active Pol II transcription sites and at some Pol III-transcribed genes, as demonstrated microscopically on the polytene chromosomes of salivary glands and by ChIP-seq analysis in S2 cells. Intron recognition seems to interfere with association and translocation of UPF1 on nascent pre-mRNAs, and cells depleted of UPF1 show defects in the release of mRNAs from transcription sites and mRNA export from the nucleus.",
keywords = "D. melanogaster, NMD, RNA helicases, Transcription, chromosomes, gene expression, mRNA export, polytene chromosomes, splicing",
author = "Anand Singh and {Roy Choudhury}, Subhendu and Sandip De and Jie Zhang and Stephen Kissane and Vibha Dwivedi and Preethi Ramanathan and Marija Petric and Luisa Orsini and Daniel Hebenstreit and Saverio Brogna",
note = "{\textcopyright} 2019, Singh et al.",
year = "2019",
month = mar,
day = "25",
doi = "10.7554/eLife.41444",
language = "English",
volume = "8",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
number = "8",

}

RIS

TY - JOUR

T1 - The RNA helicase UPF1 associates with mRNAs co-transcriptionally and is required for the release of mRNAs from gene loci

AU - Singh, Anand

AU - Roy Choudhury, Subhendu

AU - De, Sandip

AU - Zhang, Jie

AU - Kissane, Stephen

AU - Dwivedi, Vibha

AU - Ramanathan, Preethi

AU - Petric, Marija

AU - Orsini, Luisa

AU - Hebenstreit, Daniel

AU - Brogna, Saverio

N1 - © 2019, Singh et al.

PY - 2019/3/25

Y1 - 2019/3/25

N2 - UPF1 is an RNA helicase that is required for efficient nonsense-mediated mRNA decay (NMD) in eukaryotes, and the predominant view is that UPF1 mainly operates on the 3'UTRs of mRNAs that are directed for NMD in the cytoplasm. Here we offer evidence, obtained from Drosophila, that UPF1 constantly moves between the nucleus and cytoplasm by a mechanism that requires its RNA helicase activity. UPF1 is associated, genome-wide, with nascent RNAs at most of the active Pol II transcription sites and at some Pol III-transcribed genes, as demonstrated microscopically on the polytene chromosomes of salivary glands and by ChIP-seq analysis in S2 cells. Intron recognition seems to interfere with association and translocation of UPF1 on nascent pre-mRNAs, and cells depleted of UPF1 show defects in the release of mRNAs from transcription sites and mRNA export from the nucleus.

AB - UPF1 is an RNA helicase that is required for efficient nonsense-mediated mRNA decay (NMD) in eukaryotes, and the predominant view is that UPF1 mainly operates on the 3'UTRs of mRNAs that are directed for NMD in the cytoplasm. Here we offer evidence, obtained from Drosophila, that UPF1 constantly moves between the nucleus and cytoplasm by a mechanism that requires its RNA helicase activity. UPF1 is associated, genome-wide, with nascent RNAs at most of the active Pol II transcription sites and at some Pol III-transcribed genes, as demonstrated microscopically on the polytene chromosomes of salivary glands and by ChIP-seq analysis in S2 cells. Intron recognition seems to interfere with association and translocation of UPF1 on nascent pre-mRNAs, and cells depleted of UPF1 show defects in the release of mRNAs from transcription sites and mRNA export from the nucleus.

KW - D. melanogaster

KW - NMD

KW - RNA helicases

KW - Transcription

KW - chromosomes

KW - gene expression

KW - mRNA export

KW - polytene chromosomes

KW - splicing

UR - http://www.scopus.com/inward/record.url?scp=85064184910&partnerID=8YFLogxK

U2 - 10.7554/eLife.41444

DO - 10.7554/eLife.41444

M3 - Article

C2 - 30907728

VL - 8

JO - eLife

JF - eLife

SN - 2050-084X

IS - 8

M1 - e41444

ER -