The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage

Research output: Contribution to journalArticlepeer-review

Standard

The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage. / Larsen, Dorthe H; Hari, Flurina; Clapperton, Julie A; Gwerder, Myriam; Gutsche, Katrin; Altmeyer, Matthias; Jungmichel, Stephanie; Toledo, Luis I; Fink, Daniel; Rask, Maj-Britt; Grøfte, Merete; Lukas, Claudia; Nielsen, Michael L; Smerdon, Stephen J; Lukas, Jiri; Stucki, Manuel.

In: Nature Cell Biology, Vol. 16, No. 8, 08.2014, p. 792-803.

Research output: Contribution to journalArticlepeer-review

Harvard

Larsen, DH, Hari, F, Clapperton, JA, Gwerder, M, Gutsche, K, Altmeyer, M, Jungmichel, S, Toledo, LI, Fink, D, Rask, M-B, Grøfte, M, Lukas, C, Nielsen, ML, Smerdon, SJ, Lukas, J & Stucki, M 2014, 'The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage', Nature Cell Biology, vol. 16, no. 8, pp. 792-803. https://doi.org/10.1038/ncb3007

APA

Larsen, D. H., Hari, F., Clapperton, J. A., Gwerder, M., Gutsche, K., Altmeyer, M., Jungmichel, S., Toledo, L. I., Fink, D., Rask, M-B., Grøfte, M., Lukas, C., Nielsen, M. L., Smerdon, S. J., Lukas, J., & Stucki, M. (2014). The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage. Nature Cell Biology, 16(8), 792-803. https://doi.org/10.1038/ncb3007

Vancouver

Larsen DH, Hari F, Clapperton JA, Gwerder M, Gutsche K, Altmeyer M et al. The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage. Nature Cell Biology. 2014 Aug;16(8):792-803. https://doi.org/10.1038/ncb3007

Author

Larsen, Dorthe H ; Hari, Flurina ; Clapperton, Julie A ; Gwerder, Myriam ; Gutsche, Katrin ; Altmeyer, Matthias ; Jungmichel, Stephanie ; Toledo, Luis I ; Fink, Daniel ; Rask, Maj-Britt ; Grøfte, Merete ; Lukas, Claudia ; Nielsen, Michael L ; Smerdon, Stephen J ; Lukas, Jiri ; Stucki, Manuel. / The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage. In: Nature Cell Biology. 2014 ; Vol. 16, No. 8. pp. 792-803.

Bibtex

@article{cceca954d81240f88a02418d32ddba2c,
title = "The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage",
abstract = "Chromosome breakage elicits transient silencing of ribosomal RNA synthesis, but the mechanisms involved remained elusive. Here we discover an in trans signalling mechanism that triggers pan-nuclear silencing of rRNA transcription in response to DNA damage. This is associated with transient recruitment of the Nijmegen breakage syndrome protein 1 (NBS1), a central regulator of DNA damage responses, into the nucleoli. We further identify TCOF1 (also known as Treacle), a nucleolar factor implicated in ribosome biogenesis and mutated in Treacher Collins syndrome, as an interaction partner of NBS1, and demonstrate that NBS1 translocation and accumulation in the nucleoli is Treacle dependent. Finally, we provide evidence that Treacle-mediated NBS1 recruitment into the nucleoli regulates rRNA silencing in trans in the presence of distant chromosome breaks. ",
keywords = "Amino Acid Sequence, Cell Cycle Proteins/chemistry, Cell Line, Cell Nucleolus/metabolism, Conserved Sequence, DNA Breaks, Double-Stranded, DNA Damage/genetics, Gene Silencing, Green Fluorescent Proteins/genetics, HEK293 Cells, HeLa Cells, Humans, Models, Biological, Molecular Sequence Data, Multiprotein Complexes/chemistry, Nuclear Proteins/chemistry, Phosphoproteins/chemistry, Phosphorylation, Protein Interaction Domains and Motifs, RNA Polymerase I/metabolism, RNA, Ribosomal/genetics, Recombinant Fusion Proteins/genetics, Transcription, Genetic",
author = "Larsen, {Dorthe H} and Flurina Hari and Clapperton, {Julie A} and Myriam Gwerder and Katrin Gutsche and Matthias Altmeyer and Stephanie Jungmichel and Toledo, {Luis I} and Daniel Fink and Maj-Britt Rask and Merete Gr{\o}fte and Claudia Lukas and Nielsen, {Michael L} and Smerdon, {Stephen J} and Jiri Lukas and Manuel Stucki",
year = "2014",
month = aug,
doi = "10.1038/ncb3007",
language = "English",
volume = "16",
pages = "792--803",
journal = "Nature Cell Biology",
issn = "1465-7392",
publisher = "Nature Publishing Group",
number = "8",

}

RIS

TY - JOUR

T1 - The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage

AU - Larsen, Dorthe H

AU - Hari, Flurina

AU - Clapperton, Julie A

AU - Gwerder, Myriam

AU - Gutsche, Katrin

AU - Altmeyer, Matthias

AU - Jungmichel, Stephanie

AU - Toledo, Luis I

AU - Fink, Daniel

AU - Rask, Maj-Britt

AU - Grøfte, Merete

AU - Lukas, Claudia

AU - Nielsen, Michael L

AU - Smerdon, Stephen J

AU - Lukas, Jiri

AU - Stucki, Manuel

PY - 2014/8

Y1 - 2014/8

N2 - Chromosome breakage elicits transient silencing of ribosomal RNA synthesis, but the mechanisms involved remained elusive. Here we discover an in trans signalling mechanism that triggers pan-nuclear silencing of rRNA transcription in response to DNA damage. This is associated with transient recruitment of the Nijmegen breakage syndrome protein 1 (NBS1), a central regulator of DNA damage responses, into the nucleoli. We further identify TCOF1 (also known as Treacle), a nucleolar factor implicated in ribosome biogenesis and mutated in Treacher Collins syndrome, as an interaction partner of NBS1, and demonstrate that NBS1 translocation and accumulation in the nucleoli is Treacle dependent. Finally, we provide evidence that Treacle-mediated NBS1 recruitment into the nucleoli regulates rRNA silencing in trans in the presence of distant chromosome breaks.

AB - Chromosome breakage elicits transient silencing of ribosomal RNA synthesis, but the mechanisms involved remained elusive. Here we discover an in trans signalling mechanism that triggers pan-nuclear silencing of rRNA transcription in response to DNA damage. This is associated with transient recruitment of the Nijmegen breakage syndrome protein 1 (NBS1), a central regulator of DNA damage responses, into the nucleoli. We further identify TCOF1 (also known as Treacle), a nucleolar factor implicated in ribosome biogenesis and mutated in Treacher Collins syndrome, as an interaction partner of NBS1, and demonstrate that NBS1 translocation and accumulation in the nucleoli is Treacle dependent. Finally, we provide evidence that Treacle-mediated NBS1 recruitment into the nucleoli regulates rRNA silencing in trans in the presence of distant chromosome breaks.

KW - Amino Acid Sequence

KW - Cell Cycle Proteins/chemistry

KW - Cell Line

KW - Cell Nucleolus/metabolism

KW - Conserved Sequence

KW - DNA Breaks, Double-Stranded

KW - DNA Damage/genetics

KW - Gene Silencing

KW - Green Fluorescent Proteins/genetics

KW - HEK293 Cells

KW - HeLa Cells

KW - Humans

KW - Models, Biological

KW - Molecular Sequence Data

KW - Multiprotein Complexes/chemistry

KW - Nuclear Proteins/chemistry

KW - Phosphoproteins/chemistry

KW - Phosphorylation

KW - Protein Interaction Domains and Motifs

KW - RNA Polymerase I/metabolism

KW - RNA, Ribosomal/genetics

KW - Recombinant Fusion Proteins/genetics

KW - Transcription, Genetic

U2 - 10.1038/ncb3007

DO - 10.1038/ncb3007

M3 - Article

C2 - 25064736

VL - 16

SP - 792

EP - 803

JO - Nature Cell Biology

JF - Nature Cell Biology

SN - 1465-7392

IS - 8

ER -