The mycobacterial receptor, Clec4d (CLECSF8, MCL) is co-regulated with Mincle and upregulated on mouse myeloid cells following microbial challenge

Research output: Contribution to journalArticlepeer-review


  • Bernhard Kerscher
  • Gillian J Wilson
  • Delyth M Reid
  • Daiki Mori
  • Julie A Taylor
  • Sho Yamasaki
  • Janet A Willment
  • Gordon D Brown

Colleges, School and Institutes

External organisations

  • Univ Aberdeen
  • University of Aberdeen


The C-type lectin receptor (CTLR), Clec4d (MCL, CLECSF8), is a member of the Dectin-2 cluster of CTLRs, which also includes the related receptors Mincle and Dectin-2. Like Mincle, Clec4d recognises mycobacterial cord factor, trehalose dimycolate, and we recently demonstrated its key role in anti-mycobacterial immunity in mouse and man. Here, we characterised receptor expression in naïve mice, under inflammatory conditions, and during Mycobacterium bovis BCG infection using newly generated monoclonal antibodies. In naïve mice, Clec4d was predominantly expressed on myeloid cells within the peritoneal cavity, blood and bone marrow. Unexpectedly, basal expression of Clec4d was very low on leukocytes in the lung. However, receptor expression was significantly upregulated on pulmonary myeloid cells during Mycobacterium bovis BCG infection. Moreover, Clec4d expression could be strongly induced in vitro and in vivo by various microbial stimuli, including TLR agonists, but not exogenous cytokines. Notably, we show that Clec4d requires association with the signalling adaptor FcRγ and Mincle, but not Dectin-2, for surface expression. In addition, we provide evidence that Clec4d and Mincle, but not Dectin-2, are interdependently co-regulated during inflammation and infection. These data show that Clec4d is an inducible myeloid-expressed CTLR in mice, whose expression is tightly linked to that of Mincle. This article is protected by copyright. All rights reserved.


Original languageEnglish
JournalEuropean Journal of Immunology
Early online date8 Dec 2015
Publication statusE-pub ahead of print - 8 Dec 2015


  • C-type lectin receptor, CLECSF8, CLEC4E, CLEC4N, Innate immunity, MCL, Mycobacterium bovis BCG