The microRNA-30 family targets DLL4 to modulate endothelial cell behavior during angiogenesis

Research output: Contribution to journalArticlepeer-review

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The microRNA-30 family targets DLL4 to modulate endothelial cell behavior during angiogenesis. / Bridge, Gemma; Monteiro, Rui; Henderson, Stephen; Emuss, Victoria; Lagos, Dimitris; Georgopoulou, Dimitra; Patient, Roger; Boshoff, Chris.

In: Blood, Vol. 120, No. 25, 13.12.2012, p. 5063-5072.

Research output: Contribution to journalArticlepeer-review

Harvard

Bridge, G, Monteiro, R, Henderson, S, Emuss, V, Lagos, D, Georgopoulou, D, Patient, R & Boshoff, C 2012, 'The microRNA-30 family targets DLL4 to modulate endothelial cell behavior during angiogenesis', Blood, vol. 120, no. 25, pp. 5063-5072. https://doi.org/10.1182/blood-2012-04-423004

APA

Bridge, G., Monteiro, R., Henderson, S., Emuss, V., Lagos, D., Georgopoulou, D., Patient, R., & Boshoff, C. (2012). The microRNA-30 family targets DLL4 to modulate endothelial cell behavior during angiogenesis. Blood, 120(25), 5063-5072. https://doi.org/10.1182/blood-2012-04-423004

Vancouver

Author

Bridge, Gemma ; Monteiro, Rui ; Henderson, Stephen ; Emuss, Victoria ; Lagos, Dimitris ; Georgopoulou, Dimitra ; Patient, Roger ; Boshoff, Chris. / The microRNA-30 family targets DLL4 to modulate endothelial cell behavior during angiogenesis. In: Blood. 2012 ; Vol. 120, No. 25. pp. 5063-5072.

Bibtex

@article{e52e14edf5914ccaa43368fd505321b6,
title = "The microRNA-30 family targets DLL4 to modulate endothelial cell behavior during angiogenesis",
abstract = "Delta-like 4 (DLL4), a membrane-bound ligand belonging to the Notch signaling family, plays a fundamental role in vascular development and angiogenesis. We identified a conserved microRNA family, miR-30, which targets DLL4. Overexpression of miR-30b in endothelial cells led to increased vessel number and length in an in vitro model of sprouting angiogenesis. Microinjection of miR-30 mimics into zebrafish embryos resulted in suppression of dll4 and subsequent excessive sprouting of intersegmental vessels and reduction in dorsal aorta diameter. Use of a target protector against the miR-30 site within the dll4 3′UTR up-regulated dll4 and synergized with Vegfa signaling knockdown to inhibit angiogenesis. Furthermore, restoration of miR-30b or miR- 30c expression during Kaposi sarcoma herpesvirus (KSHV) infection attenuated viral induction of DLL4. Together these results demonstrate that the highly conserved molecular targeting of DLL4 by the miR-30 family regulates angiogenesis.",
author = "Gemma Bridge and Rui Monteiro and Stephen Henderson and Victoria Emuss and Dimitris Lagos and Dimitra Georgopoulou and Roger Patient and Chris Boshoff",
year = "2012",
month = dec,
day = "13",
doi = "10.1182/blood-2012-04-423004",
language = "English",
volume = "120",
pages = "5063--5072",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "25",

}

RIS

TY - JOUR

T1 - The microRNA-30 family targets DLL4 to modulate endothelial cell behavior during angiogenesis

AU - Bridge, Gemma

AU - Monteiro, Rui

AU - Henderson, Stephen

AU - Emuss, Victoria

AU - Lagos, Dimitris

AU - Georgopoulou, Dimitra

AU - Patient, Roger

AU - Boshoff, Chris

PY - 2012/12/13

Y1 - 2012/12/13

N2 - Delta-like 4 (DLL4), a membrane-bound ligand belonging to the Notch signaling family, plays a fundamental role in vascular development and angiogenesis. We identified a conserved microRNA family, miR-30, which targets DLL4. Overexpression of miR-30b in endothelial cells led to increased vessel number and length in an in vitro model of sprouting angiogenesis. Microinjection of miR-30 mimics into zebrafish embryos resulted in suppression of dll4 and subsequent excessive sprouting of intersegmental vessels and reduction in dorsal aorta diameter. Use of a target protector against the miR-30 site within the dll4 3′UTR up-regulated dll4 and synergized with Vegfa signaling knockdown to inhibit angiogenesis. Furthermore, restoration of miR-30b or miR- 30c expression during Kaposi sarcoma herpesvirus (KSHV) infection attenuated viral induction of DLL4. Together these results demonstrate that the highly conserved molecular targeting of DLL4 by the miR-30 family regulates angiogenesis.

AB - Delta-like 4 (DLL4), a membrane-bound ligand belonging to the Notch signaling family, plays a fundamental role in vascular development and angiogenesis. We identified a conserved microRNA family, miR-30, which targets DLL4. Overexpression of miR-30b in endothelial cells led to increased vessel number and length in an in vitro model of sprouting angiogenesis. Microinjection of miR-30 mimics into zebrafish embryos resulted in suppression of dll4 and subsequent excessive sprouting of intersegmental vessels and reduction in dorsal aorta diameter. Use of a target protector against the miR-30 site within the dll4 3′UTR up-regulated dll4 and synergized with Vegfa signaling knockdown to inhibit angiogenesis. Furthermore, restoration of miR-30b or miR- 30c expression during Kaposi sarcoma herpesvirus (KSHV) infection attenuated viral induction of DLL4. Together these results demonstrate that the highly conserved molecular targeting of DLL4 by the miR-30 family regulates angiogenesis.

UR - http://www.scopus.com/inward/record.url?scp=84871038819&partnerID=8YFLogxK

U2 - 10.1182/blood-2012-04-423004

DO - 10.1182/blood-2012-04-423004

M3 - Article

C2 - 23086751

AN - SCOPUS:84871038819

VL - 120

SP - 5063

EP - 5072

JO - Blood

JF - Blood

SN - 0006-4971

IS - 25

ER -